IP Library › Granted Patent US 11,965,185
Granted Patent B2
US 11,965,185 · App. 17/245,401 · Granted Apr 23, 2024

Patent

Inventors: Liyang Zhang (North Liberty, IA); Christopher Anthony Vakulskas (North Liberty, IA)
Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
C12N9/22
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Quick Facts
Patent No.
US 11,965,185
App. No.
17/245,401
Granted
Apr 23, 2024
Kind
B2
Abstract

Described herein are CAS12A mutants from Lachnospiraceae bacterium and methods for use thereof. These mutants have enhanced DNA cleavage activities at non-canonical TTTT protospacer adjacent motifs (PAM) compared to the wild-type enzyme.

Claims (2)

1. An isolated mutant Lachnospiraceae bacterium ND2006 Cas12a (“LbCas12a”) polypeptide comprising the G146R/R182V/E795Q polypeptide sequence of SEQ ID NO: 39844.

2. An isolated polynucleotide sequence encoding the mutant LbCas12a polypeptide of claim 1 , wherein the polynucleotide sequence comprises the sequence of SEQ ID NO: 3983.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 17, 2023
From: ZHANG, LIYANG; VAKULSKAS, CHRISTOPHER ANTHONY
To: INTEGRATED DNA TECHNOLOGIES, INC.
Reel/Frame 063346/0703 →
Continuity (3)
Provisional Application 63090912 · Oct 13, 2020
Provisional Application 63018592 · May 1, 2020
Related Publication 20210348144A1 · Nov 11, 2021