Immunogenic and vaccine compositions against SARS-CoV-2
View Patent ↗Disclosed herein are immunogenic and/or vaccine compositions and methods for treating or preventing Severe acute respiratory syndrome (SARS). The compositions and methods include an immunogenic portion of the receptor-binding domain (RBD) of the SARS-CoV-2-2 (COVID-19) spike protein. In at least particular cases, a mutated version of a portion of the RBD is utilized, such as a deglycosylated, or amino acid substituted mutant of the spike protein.
1. An immunogenic composition comprising:
a fragment of the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein, wherein the fragment is the receptor-binding domain (RBD) as set forth in SEQ ID NO: 45, and wherein the RBD is N-glycosylated;
an adjuvant; and
a carrier.
2. The composition of claim 1 , wherein the immunogenic composition induces neutralizing antibodies against SARS-CoV-2.
3. An immunogenic composition comprising:
a polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 3, wherein the polypeptide is N-glycosylated;
an adjuvant; and
a carrier.
4. The composition of claim 1 , wherein said fragment comprises SEQ ID NO: 4.
5. The composition of claim 1 , wherein said fragment comprises one or more amino acid substitutions so that said fragment is not naturally occurring.
6. A method of preventing or delaying the onset of Coronavirus Disease 2019 (COVID-19) or of reducing at least one symptom of COVID- 19 in a subject, comprising the step of providing an effective amount of the composition of claim 1 to the subject.
7. The method of claim 6 , wherein the composition is provided to the subject once.
8. The method of claim 6 , wherein the composition is provided to the subject more than once.
9. The method of claim 6 , wherein the composition is provided subsequently to the subject within weeks, months, or years of the first providing step.
10. The method of claim 6 , wherein the subject displays one or more symptoms of COVID-19.
11. The method of claim 6 , wherein the subject lacks any symptoms of COVID-19.
12. The method of claim 6 , wherein the subject has been exposed to COVID-19.
13. The method of claim 6 , wherein the subject has come into contact with a subject that has COVID-19.
14. The method of claim 6 , wherein the subject is a child, an elderly person, exposed to a bioweapon or at risk thereof, is a member of the military, or is a health care worker.