Fluorescent probe and protein labeling method
Provided are a fluorescent probe, a preparation method therefor and a use thereof. The fluorescent probe sensitively and specifically responds to viscosity, and can be used for the specific fluorescence labeling of proteins as well as in the quantification, detection or kinetic study of proteins and the imaging of cells, tissues and living bodies.
1 . A fluorescent probe
having a structure represented by formula (I):
wherein:
the A is selected from the following structures:
the C is —(C═O)—O—CH 2 —;
the D is
X 1 is selected from hydrogen and a C 1 -C 30 linear or branched alkyl, X 2 is a C 1 -C 30 linear or branched alkylene;
the B has any one of structures represented by formula (I-1-1) to (I-1-2) and formula (I-1-4) to (I-1-7):
a structural part of formula (I) as shown in formula (I-2) has any one of the following formula (I-2-1) to (I-2-11), (I-2-13), (I-2-15) and (I-2-18):
2 . The fluorescent probe according to claim 1 , wherein the fluorescent probe has a structure selected from a group consisting of
3 . The fluorescent probe according to claim 1 , wherein X 1 is a C 1-10 linear or branched alkyl group, and X 2 is a C 1-10 linear or branched chain alkylene group.
4 . A probe kit comprising the fluorescent probe according to claim 1 .
5 . The probe kit according to claim 4 , wherein, said probe kit further comprises a biocompatible medium.
6 . The probe kit according to claim 5 , wherein, said biocompatible medium is at least one selected from dimethyl sulfoxide, a buffer, and physiological saline.
7 . The probe kit according to claim 6 , wherein, said buffer includes phosphate buffer.
8 . A fluorescent activated protein specific labeling method, comprising steps of:
contacting the fluorescent probe according to claim 1 with a target protein having a protein tag; and
performing a labeling reaction between the fluorescent probe and the protein tag to label the protein tag with the fluorescent probe.
9 . The fluorescent activated protein specific labeling method according to claim 8 , wherein, the labeling of the protein tag with the fluorescent probe is covalently labeling; and a reaction medium of said labeling reaction is selected from a pure protein solution, a cell lysate and an in situ medium in which the target protein having a protein tag is located.
10 . The fluorescent activated protein specific labeling method according to claim 9 , wherein, the in situ medium is selected from intracellular media, organelle media, living tissue media, blood, and body fluids.
11 . A method for quantification, detection or kinetic studies of proteins, or for imaging of cells, tissues, and living bodies, comprising a step of using the fluorescent probe of claim 1 to quantify proteins, detect proteins, or perform kinetic studies of proteins or to image cells, image tissues, or image living bodies.