Methods of preparing mammalian enteroids, and methods producing T. gondii oocysts in vitro and in vivo in heterologous systems, are provided.
1. A method to generate an immune response to Toxoplasma in livestock, comprising: orally administering to said livestock an effective amount of isolated infectious Toxoplasma oocysts so as to generate an immune response to Toxoplasma, wherein the Toxoplasma oocysts are prepared in vitro.
2. The method of claim 1 , wherein the livestock is a sheep, goat or swine.
3. The method of claim 1 wherein the oocysts are resistant to 1% bleach but not to 10% bleach.
4. The method of claim 1 wherein the oocysts are orally, subcutaneously or intraperitoneally administered.
5. The method of claim 1 wherein the oocysts are injected.
6. The method of claim 1 , wherein the Toxoplasma is genetically modified to inhibit tachyzoite formation.