Fusion fragment chimeric antigen receptors and uses thereof
View Patent ↗Disclosed herein are fusion fragment chimeric antigen receptors (FF-CARs), engineered cells expressing FF-CARs, and methods of using thereof to treat, reduce, or inhibit infections by enveloped viruses in subjects.
1 . An expression vector comprising a nucleic acid sequence that encodes a chimeric antigen receptor consisting of an amino acid sequence as set forth in SEQ ID NO: 18 or SEQ ID NO: 20.
2 . An expression vector comprising a nucleic acid sequence that encodes a chimeric antigen receptor, wherein the nucleic acid sequence is SEQ ID NO: 17 or SEQ ID NO: 19.
3 . A cell comprising an expression vector according to claim 1 .
4 . The cell according to claim 3 , wherein the cell is a B cell, a T cell, a natural killer T cell, a hematopoietic stem cell, or a hematopoietic progenitor cell.
5 . A cell that is the progeny of the cell of claim 3 .
6 . The cell according to claim 3 , wherein the cell expresses the chimeric antigen receptor encoded by the expression vector.
7 . A method of treating, reducing, or inhibiting HIV-1 in a subject, which comprises transplanting one or more cells according to claim 3 , in the subject.
8 . A method of killing an infected cell infected with HIV-1, which comprises contacting the infected cell with one or more cells according to claim 3 .
9 . The method according to claim 7 , wherein the subject is human.
10 . A cell comprising an expression vector according to claim 2 .
11 . The cell according to claim 10 , wherein the cell is a B cell, a T cell, a natural killer T cell, a hematopoietic stem cell, or a hematopoietic progenitor cell.
12 . A cell that is the progeny of the cell of claim 11 .
13 . The cell according to claim 12 , wherein the cell expresses the chimeric antigen receptor encoded by the expression vector.
14 . A chimeric antigen receptor encoded by the expression vector according to claim 1 .
15 . A chimeric antigen receptor encoded by the expression vector according to claim 2 .