Method for preparing iridoid-rich noni fruit extract or fraction thereof, method for preparing immune-enhancing active material-rich noni fruit extract or fraction thereof and use of noni fruit extract or fraction thereof
The present invention relates to a method of preparing a noni fruit extract containing a high iridoid content or a fraction thereof, a method of preparing a noni fruit extract containing a high content of an immune-enhancing active substance or a fraction thereof, and a use of a noni fruit extract or a fraction thereof, and more specifically, the present invention provides a method of preparing a noni fruit extract containing a high iridoid content or a fraction thereof, including preparing a noni fruit extract by using ethanol in a specific concentration range as an extraction solvent, a method of preparing a noni fruit extract containing a high content of an immune-enhancing active substance or a fraction thereof, a pharmaceutical composition for enhancing immunity including the extract or a fraction thereof, a health functional food composition including the same constitution, a method of enhancing immunity using the extract or a fraction thereof, and a use of the extract or a fraction thereof in preparation of medicine for preventing or treating a disease associated with immune enhancement or reduced immunity.
1. A method of enhancing immunity, comprising administering a noni fruit extract or a purified water fraction thereof to a subject in need thereof at an effective amount, wherein the extract is prepared by using 10 (w/w) % ethanol as an extraction solvent, and the ethanol extract and the purified water fraction thereof comprise a polysaccharide having a mean molecular weight of 10 kDa or more.
2. The method of claim 1 , wherein the extract is prepared by the following steps of:
a) pulverizing noni fruit;
b) injecting 10 (w/w) % ethanol of 5 to 15 times the weight of the pulverized product into the pulverized product; and
c) preparing an extract by extracting at 60° C. to 80° C. for 1 hour to 8 hours.
3. The method of claim 1 , wherein the fraction is prepared by the following steps of:
a) pulverizing noni fruit;
b) injecting 10 (w/w) % ethanol of 5 to 15 times the weight of the pulverized product into the pulverized product;
c) preparing an extract by extracting at 60° C. to 80° C. for 1 hour to 8 hours; and
d) fractionating the obtained extract with purified water.
4. The method of claim 1 , wherein an immune enhancing active substance of the noni fruit extract or the fraction thereof is an iridoid and a polysaccharide having a molecular weight of 10 kDa or more.
5. The method of claim 4 , wherein the iridoid is one or more selected from the group consisting of deacetylasperulosidic acid (DAA), asperulosidic acid (ASPA) and asperuloside (ASP).