Low endotoxin fucan compositions, systems, and methods
Low endotoxin fucan compositions comprising a therapeutically effective, medically acceptable fucan in a composition comprising less than about 0.2, 0.18, 0.1, 0.01, 0.001, or 0.0005 endotoxin units (EU) per milligram of the fucan are disclosed. Methods and systems for removing or reducing the amount of endotoxins from a starting fucan composition are also disclosed.
1. A fucan-low endotoxin composition comprising less than about 0.2 endotoxin units per milligram of the fucan, wherein the fucan comprises an acetyl content of substantially 0% w/w when measured by 2D 1H-13C heteronuclear multiple quantum coherence at 70° C. with solvent signal suppression on a 600 MHz spectrometer equipped with 5-mm cold probe, in the range from 10-30 ppm in the carbon dimensions, in 8 increments of 256-512 scans each.
2. The fucan-low endotoxin composition of claim 1 wherein the fucan-low endotoxin composition comprises less than about 0.09 endotoxin units per milligram of the fucan, and wherein the fucan comprises an acetyl content of substantially 0% w/w when measured by 2D 1H-13C heteronuclear multiple quantum coherence at 70° C. with solvent signal suppression on a 600 MHz spectrometer equipped with 5-mm cold probe, in the range from 10-30 ppm in the carbon dimensions, in 8 increments of 256-512 scans each.
3. The fucan-low endotoxin composition of claim 1 wherein the fucan-low endotoxin composition comprises less than about 0.03 endotoxin units per milligram of the fucan, and wherein the fucan comprises an acetyl content of substantially 0% w/w when measured by 2D 1H-13C heteronuclear multiple quantum coherence at 70° C. with solvent signal suppression on a 600 MHz spectrometer equipped with 5-mm cold probe, in the range from 10-30 ppm in the carbon dimensions, in 8 increments of 256-512 scans each.
4. The fucan-low endotoxin composition of claim 1 wherein the fucan-low endotoxin composition comprises less than about 0.003 endotoxin units per milligram of the fucan, and wherein the fucan comprises an acetyl content of substantially 0% w/w when measured by 2D 1H-13C heteronuclear multiple quantum coherence at 70° C. with solvent signal suppression on a 600 MHz spectrometer equipped with 5-mm cold probe, in the range from 10-30 ppm in the carbon dimensions, in 8 increments of 256-512 scans each.
5. The fucan-low endotoxin composition of claim 1 wherein the fucan-low endotoxin composition comprises less than about 0.0005 endotoxin units per milligram of the fucan, and wherein the fucan comprises an acetyl content of substantially 0% w/w when measured by 2D 1H-13C heteronuclear multiple quantum coherence at 70° C. with solvent signal suppression on a 600 MHz spectrometer equipped with 5-mm cold probe, in the range from 10-30 ppm in the carbon dimensions, in 8 increments of 256-512 scans each.