IP Library Granted Patent US 11,548,875
Granted Patent B2
US 11,548,875 · App. 17/262,877 · Granted Jan 10, 2023

Inhibitor compounds for male contraception

Inventors: Alex S. Goldstein (Seattle, WA); John K. Amory (Seattle, WA); Jisun Paik (Seattle, WA); Michael Haenisch (Seattle, WA); Nina Isoherranen (Seattle, WA); Piper Treuting (Seattle, WA); Charles H. Muller (Seattle, WA)
Assignee: University of Washington
C07D403/12A61K9/0024A61P15/16C07D231/14
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Quick Facts
Patent No.
US 11,548,875
App. No.
17/262,877
Granted
Jan 10, 2023
Kind
B2
Abstract

Pyrazole compounds and piperazine compounds that are inhibitors of ALDH1A1 and ALDH1A2 and methods for using the pyrazole compounds and piperazine compounds in male contraceptive compositions for preventing spermatogenesis.

Claims (68)

1. A compound having formula (I):

or a pharmaceutically acceptable salt thereof, wherein

R 1 is selected from the group consisting of:

(a) NO 2 ,

(b) SO 2 R a , wherein R a is C1-C6 linear or branched alkyl or alkenyl,

(c) C(═O)R a , wherein R a is hydrogen, C1-C6 linear or branched alkyl or alkenyl, optionally substituted with one or two halo groups, or NR c R d , wherein R c and R d are independently selected from hydrogen and C1-C3 linear or branched alkyl, and

(d) NH(R e ) or N(R a )(R e ), wherein R a is C1-C6 linear or branched alkyl, and wherein R e is selected from

(i) hydrogen,

(ii) C(═O)H,

(iii) C(═O)R f , wherein R f is C1-C6 linear or branched alkyl or cyclo alkyl, optionally substituted with one or two halo groups,

(iv) C(═O)(CH 2 ) n OH, wherein n is 1, 2, 3, or 4, or

(v) SO 2 R g , wherein R g is C1-C6 linear or branched alkyl;

R 2 is selected from the group consisting of:

(a) phenyl optionally substituted with

(i) C(═O)R a , wherein R a is C1-C6 linear or branched alkyl, or R a is NR c R d , wherein R c and R d are independently selected from is hydrogen and C1-C3 linear or branched alkyl, or R c is hydrogen and R d is hydroxy,

(ii) CO 2 R b , wherein R b is hydrogen, or

(iii) cyano,

(b) C1-C6 linear or branched alkyl, and

(c) C1-C6 linear or branched haloalkyl;

X is NH; and

Y is m-fluoro phenyl.

2. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 1 is C(═O)R a , wherein R a is C1-C6 linear or branched alkyl or alkenyl, optionally substituted with one or two halo groups, or NH(C═O)R f , wherein R f is C1-C6 linear or branched alkyl, optionally substituted with one or two halo groups.

3. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 1 is C(═O)R a , wherein R a is C1-C6 linear or branched alkyl.

4. The compound of claim 1 , or a pharmaceutically acceptable salt thereof, wherein R 2 is selected from phenyl optionally substituted with cyano or CO 2 R a , wherein R a is C1-C6 linear or branched alkyl.

5. A compound having formula (IV):

or a pharmaceutically acceptable salt thereof, wherein

R 1 is N-methyl pyrrolyl;

R 2 and R 3 taken together with the nitrogen atom to which they are attached form a 4-, 5-, 6-, or 7-membered ring; and

R 4 is selected from the group consisting of:

(a) NO 2 ,

(b) NH(R e ), wherein R e is

C(═O)R f , wherein R f is C1-C6 linear or branched alkyl, optionally substituted with one or two halo groups, and

(c) N(R g )C(═O)R f , wherein R g is C1-C6 linear or branched alkyl, and R f is C1-C6 linear or branched alkyl optionally substituted with one or two halo groups.

6. A compound of claim 1 selected from the group consisting of:

N-(3-fluorophenyl)-3-(4-nitrophenyl)-1-phenyl-1H-pyrazole-4-carboxamide;

3-(4-(methylsulfonyl)phenyl)-N-(3-fluorophenyl)-1-phenyl-1H-pyrazole-4-carboxamide;

3-(4-(ethylsulfonyl)phenyl)-N-(3-fluorophenyl)-1-phenyl-1H-pyrazole-4-carboxamide;

N-(4-cyanophenyl)-3-[4-(methylsulfonyl)phenyl]-1-(3-fluorophenyl)-1H-pyrazole-4-carboxamide;

1-(4-formylphenyl)-3-[4-(methylsulfonyl)phenyl]-N-(3-fluorophenyl)-1H-pyrazole-4-carboxamide;

3-{4-dichloroacetyl-N-methyl-aminophenyl}-N-(3-fluorophenyl)-1-phenyl-1H-pyrazole-4-carboxamide;

3-[4-(dichloroacetyl)phenyl]-N-(3-fluorophenyl)-1-phenyl-1H-pyrazole-4-carboxamide;

3-{[(dichloroacetyl)(ethyl)amino]methyl}-N-(3-fluorophenyl)-1-phenyl-1H-pyrazole-4-carboxamide; and

pharmaceutically acceptable salts thereof.

7. A pharmaceutical composition comprising a compound of claim 1 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

8. An implantable device comprising a compound of claim 1 , or a pharmaceutically acceptable salt thereof.

9. A method for inhibiting ALDH1A1 and ALDH1A2 in a subject, comprising administering a therapeutically effective amount of a compound of claim 1 , or a pharmaceutically acceptable salt thereof, to subject in need thereof.

10. A method for preventing spermatogenesis in a subject, comprising administering a therapeutically effective amount of a compound of claim 1 , or a pharmaceutically acceptable salt thereof, to subject in need thereof.

11. The method of claim 9 , wherein the compound, or a pharmaceutically acceptable salt thereof, is administered systemically, intravenously, subcutaneously, intramuscularly, or topically.

12. The method of claim 9 , wherein the compound, or a pharmaceutically acceptable salt thereof, is administered by release from an implantable device.

13. The method of claim 10 , wherein the compound, or a pharmaceutically acceptable salt thereof, is administered systemically, intravenously, subcutaneously, intramuscularly, or topically.

14. The method of claim 10 , wherein the compound, or a pharmaceutically acceptable salt thereof, is administered by release from an implantable device.

15. A compound of claim 5 selected from the group consisting of:

1-[4-(4-N-dichloroacetyl-3-(pyrrolidin-1-yl)phenyl]piperazin-1-yl](1-methyl-1H-pyrrol-2-yl)methanone;

1-[4-(4-N-chloroacetyl-3-(pyrrolidin-1-yl)phenyl]piperazin-1-yl](1-methyl-1H-pyrrol-2-yl)methanone;

1-[4-(4-nitrophenyl)-3-(azepan-1-yl)phenyl]piperazin-1-yl](1-methyl-1H-pyrrol-2-yl)methanone;

{4-[4-amino-3-(azepan-1-yl)phenyl]piperazin-1-yl}(1-methyl-1H-pyrrol-2-yl)methanone;

{4-[4-dichloroacetamido-3-(piperidin-1-yl)phenyl]piperazin-1-yl}(1-methyl-1H-pyrrol-2-yl)methanone;

{4-[4-dichloroacetamido-3-(azepan-1-yl)phenyl]piperazin-1-yl}(1-methyl-1H-pyrrol-2-yl)methanone;

1-[4-(4-nitrophenyl)-3-(azocan-1-yl)]piperazin-1-yl](1-methyl-1H-pyrrol-2-yl)methanone; and

pharmaceutically acceptable salts thereof.

16. A pharmaceutical composition comprising a compound of claim 5 , or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

17. An implantable device comprising a compound of claim 5 , or a pharmaceutically acceptable salt thereof.

18. A method for inhibiting ALDH1A1 and ALDH1A2 in a subject, comprising administering a therapeutically effective amount of a compound of claim 5 , or a pharmaceutically acceptable salt thereof, to subject in need thereof.

19. A method for preventing spermatogenesis in a subject, comprising administering a therapeutically effective amount of a compound of claim 5 , or a pharmaceutically acceptable salt thereof, to subject in need thereof.

20. The method of claim 18 , wherein the compound, or a pharmaceutically acceptable salt thereof, is administered systemically, intravenously, subcutaneously, intramuscularly, or topically.

21. The method of claim 18 , wherein the compound, or a pharmaceutically acceptable salt thereof, is administered by release from an implantable device.

22. The method of claim 19 , wherein the compound, or a pharmaceutically acceptable salt thereof, is administered systemically, intravenously, subcutaneously, intramuscularly, or topically.

23. The method of claim 19 , wherein the compound, or a pharmaceutically acceptable salt thereof, is administered by release from an implantable device.

Assignments (2)
CONFIRMATORY LICENSE Recorded Nov 15, 2023
From: UNIVERSITY OF WASHINGTON
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 065593/0688 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 25, 2021
From: GOLDSTEIN, ALEX S.; AMORY, JOHN K.; PAIK, JISUN; HAENISCH, MICHAEL; ISOHERRANEN, NINA; TREUTING, PIPER; MULLER, CHARLES H.
To: UNIVERSITY OF WASHINGTON
Reel/Frame 055712/0245 →
Continuity (3)
Provisional Application 62778773 · Dec 12, 2018
Provisional Application 62778786 · Dec 12, 2018
Related Publication 20210332030A1 · Oct 28, 2021