Cell culture chip and cell culture method using the same
A cell culture chip that enables observation of interactions between cells with a direction without additionally using a driving mechanism is provided. A cell culture chip includes a first culture chamber capable of culturing a cell; a second culture chamber capable of culturing a cell independently from the first culture chamber; a first well connected to the first culture chamber and capable of storing a liquid; a second well connected to the second culture chamber and being capable of storing a liquid; and a connecting portion that, when a liquid level of a first liquid stored in the first well reaches a predetermined height position or higher, allows the first liquid to flow from the first well into the second well.
1. A cell culture chip comprising:
a base plate;
a main body having tubular recesses and walls located above the base plate, the main body and the base plate together forming a first culture chamber, a second culture chamber, a first well, a second well, a third well, and a fourth well all for storing at least liquid; and
a connector having at most a second predetermined height located above the base plate between the first well and the second well;
wherein the first culture chamber and the second culture chamber are each one of the tubular recesses having a third predetermined height for culturing cells and located in the main body bellow the walls with a recess bottom surface which the base plate forms,
wherein the first well, the second well, the third well and the fourth well are each separately formed by the walls having a first predetermined height of the main body with a well bottom surface which the base plate forms,
Wherein one end of the first culture chamber is connected to the first well while the other end of the first culture chamber is connected to the third well, one end of the second culture chamber is connected to the second well while the other end of the second culture chamber is connected to the fourth well,
whereas at most the second predetermined height is shorter than the first predetermined height and taller than the third predetermined height so that the connector allows a flow of the liquid between the first well and the second well as the liquid accumulates in the first well over the connector.
2. The cell culture chip according to claim 1 ,
wherein the first well and the second well are adjacent to each other via a partition wall, and
wherein the connector is constituted by the partition wall.
3. The cell culture chip according to claim 2 , wherein the partition wall includes a cutout portion whose height from a bottom surface is at most the second predetermined height but varies in accordance with a position of the cutout portion.
4. The cell culture chip according to claim 1 , wherein a bottom surface of the first well, a bottom surface of the first culture chamber, a bottom surface of the third well, a bottom surface of the second well, a bottom surface of the second culture chamber, and a bottom surface of the fourth well are all constituted by a common surface.