Stacked integrated platform architecture
Provided herein is a system comprising (1) a microelectrode array (MEA) component comprising an integrated multiplexed (MUX) logic circuit; and (2) a microprocessor, e.g., MOSFET, such as a CMOS, wherein the MEA is in electrical communication with the microprocessor such that signals produced by the microelectrodes are transmitted to the processor through the MUX. The use of a MUX reduces the number of outputs used to communicate signals from multiple microelectrodes to the microprocessor. The two components are removably engageable with each other such that after one or more uses, the engaged MEA can be removed and replaced with a new MEA, without the necessity of disposing the microprocessor.
1. A microelectrode array system comprising:
a microelectrode array (MEA) component including a plurality of exposed microelectrodes adapted to receive electrical signals from, or to transmit electrical signals to, living cells, the microelectrode array including an embedded multiplexed logic circuit (MUX);
a complementary metal-oxide-semiconductor (CMOS) component electrically connected to the MEA component;
wherein the MEA component and the CMOS component are separable from each other;
the MEA component comprises one or more living cells which express odorant receptors, and the system is configured to perform odorant detection.
2. The system of claim 1 wherein the microelectrodes of the MEA component are associated with an interfacing component via the MUX logic circuit, and wherein the interfacing component is also associated with the CMOS component.
3. The system of claim 1 further comprising an interfacing component configured to operably connect the MEA component with the CMOS component.
4. The system of claim 3 wherein the interfacing component comprises an electrical pin, an optical element, or a combination thereof.
5. The system of claim 1 wherein the MEA component comprises at least 1,000 microelectrodes.
6. The system of claim 1 wherein the MUX is configured to consolidate inputs from the plurality of microelectrodes into a reduced number of outputs.
7. A microelectrode array system comprising:
a microelectrode array (MEA) component including a plurality of exposed microelectrodes adapted to receive electrical signals from, or to transmit electrical signals to, living cells, the microelectrode array including an embedded multiplexed logic circuit (MUX);
a complementary metal-oxide-semiconductor (CMOS) component electrically connected to the MEA component;
wherein the MEA component and the CMOS component are separable from each other, in a stacked arrangement, and a surface of the MEA component is positioned adjacent a surface of the CMOS component.
8. The system of claim 7 wherein the living cells express odorant receptors, and the system is configured to perform odorant detection.
9. A microelectrode array device comprising:
a microelectrode array (MEA) component comprising a plurality of microelectrodes in electrical contact with a surface of a substrate;
a plurality of compartments formed on or in the surface of the substrate, each compartment comprising a space in electrical contact with one or more of the plurality of microelectrodes;
one or more multiplexed (MUX) logic circuits each comprising, (1) as inputs, electrical connections with each of a plurality of the microelectrodes, (2) one or more outputs, and (3) a switch that selects between several input signals and forwards a selected input signal to an output line, wherein the total number of outputs is fewer than the total number of microelectrodes;
a plurality of living cells in each compartment, wherein the living cells express olfactory receptors.
10. The device of claim 9 further including an electrical interface configured to engage the one or more outputs, and a microprocessor, wherein the interface transmits signals from the one or more outputs to the microprocessor.
11. A method comprising:
engaging a first device to an electrical interface configured to transmit electrical signals received from the outputs of the first device to a microprocessor, the first device comprising an MEA component including a plurality of microelectrodes in electrical contact with a plurality of compartments on or in a substrate, respectively, the compartments containing living cells of the type that express odorant receptors and produce an electrical signal when exposed to a stimulus, the first device further comprising one or more multiplexed (MUX) logic circuits adapted to consolidate inputs from the plurality of microelectrodes into a reduced number of outputs to the microprocessor;
processing the electrical signals from the outputs via the microprocessor; and
disengaging the first device from the electrical interface.
12. The method of claim 11 wherein processing comprises determining an intensity of the electrical signals.
13. The method of claim 11 wherein the living cells detect one or more volatile compounds.
14. The method of claim 11 further including contacting the living cells with a gas or liquid test sample.