Transcriptional regulatory element and its use in enhancing the expression of heterologous protein
Provided is a polynucleotide, the polynucleotide can be used as a WXRE transcriptional regulatory element used to increase the protein expression level of a protein expression system. A protein expression vector or a protein expression system comprising the above-mentioned WXRE transcriptional regulatory element as well as the use thereof are also provided. The use of the WXRE transcriptional regulatory element can increase the expression level of a heterologous protein greatly with its biological activity unchanged.
1. A vector comprising a heterologous nucleotide sequence and a nucleotide sequence selected from the group consisting of (i) to (ii):
(i) a sequence of any of SEQ ID NOs: 3-9; and
(ii) a reverse complementary sequence of the sequence of any of SEQ ID NOs: 3-9.
2. The vector of claim 1 , wherein the vector is a recombinant expression vector.
3. The vector of claim 1 , further comprising a nucleotide sequence selected from the group consisting of (iii) to (iv):
(iii) a sequence having at least 80% sequence identity with SEQ ID NO: 13; and
(iv) a sequence having at least 80% sequence identity with a reverse complementary sequence of SEQ ID NO: 13.
4. The vector of claim 1 , further comprising a CMV promoter.
5. The vector of claim 4 , wherein the CMV promoter comprises a nucleotide sequence having at least 80% sequence identity with SEQ ID NO: 16.
6. The vector of claim 4 , wherein the CMV promoter comprises a nucleotide sequence having at least 90% sequence identity with SEQ ID NO: 16.
7. The vector of claim 4 , wherein the CMV promoter comprises a nucleotide sequence that is identical to SEQ ID NO: 16.
8. The vector of claim 3 , wherein the nucleotide sequence selected from the group consisting of (iii) to (iv) has at least 90% sequence identity with SEQ ID NO: 13.
9. The vector of claim 3 , wherein the nucleotide sequence selected from the group consisting of (iii) to (iv) is identical to SEQ ID NO: 13.
10. The vector of claim 1 , wherein the nucleotide sequence selected from the group consisting of (i) to (ii) is SEQ ID NO: 3.
11. The vector of claim 1 , wherein the nucleotide sequence selected from the group consisting of (i) to (ii) is SEQ ID NO: 4.
12. The vector of claim 1 , wherein the nucleotide sequence selected from the group consisting of (i) to (ii) is SEQ ID NO: 5.
13. The vector of claim 1 , wherein the nucleotide sequence selected from the group consisting of (i) to (ii) is SEQ ID NO: 6.
14. The vector of claim 1 , wherein the nucleotide sequence selected from the group consisting of (i) to (ii) is SEQ ID NO: 7.
15. The vector of claim 1 , wherein the nucleotide sequence selected from the group consisting of (i) to (ii) is SEQ ID NO: 8.
16. The vector of claim 1 , wherein the nucleotide sequence selected from the group consisting of (i) to (ii) is SEQ ID NO: 9.
17. A vector comprising a nucleotide sequence that has at least 90% sequence identity with SEQ ID NO: 13 and a nucleotide sequence selected from the group consisting of (i) to (ii):
(i) a sequence of any of SEQ ID NOs: 3-9; and
(ii) a reverse complementary sequence of the sequence of any of SEQ ID NOs: 3-9.
18. The vector of claim 17 , wherein the vector further comprises a CMV promoter.
19. A vector comprising a CMV promoter and a nucleotide sequence selected from the group consisting of (i) to (ii):
(i) a sequence of any of SEQ ID NOs: 3-9; and
(ii) a reverse complementary sequence of the sequence of any of SEQ ID NOs: 3-9.
20. The vector of claim 19 , wherein the CMV promoter comprises a nucleotide sequence having at least 80% sequence identity with SEQ ID NO: 16.