IP Library Granted Patent US 12,297,417
Granted Patent B2
US 12,297,417 · App. 17/270,167 · Granted May 13, 2025

Lateral filter array microfluidic device

Inventors: Zhonghui Hugh Fan (Gainesville, FL); Kangfu Chen (Gainesville, FL)
Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATED
C12M33/14B01D61/147B01D61/18B01D63/005C12M23/14C12M23/16C12M25/10C12M47/04G01N33/54366G01N33/57492B01D2313/08B01D2313/40
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Quick Facts
Patent No.
US 12,297,417
App. No.
17/270,167
Granted
May 13, 2025
Kind
B2
Abstract

A lateral filter array microfluidic (LFAM) device for highly efficient immunoaffinity isolation of target cells from a population of cells. The LFAM device may include of one or more serpentine main channels incorporated with lateral filter arrays. Antibodies are immobilized on the channel surface including the lateral filters and are capable of specific binding to one or more biomolecules on the surface of the target cell. The device may include one or more arrays of lateral filters with different sizes. The overall filters sizes are close to the diameter of the target cell, therefore the interaction between biomarkers on the target cells and corresponding antibodies immobilized on the filter surface is largely strengthened due to the direct contact between target cells and lateral filters. Methods include flowing a population of cells through an antibody-coated LFAM device for target cells capture, followed by washing the device to remove non-specific captured cells.

Claims (31)

1. A lateral filter array microfluidic device for capturing a target isolate in a liquid sample, the device comprising

a substrate; and

at least one series of boundaries associated with the substrate,

wherein the at least one series of boundaries are arranged to define at least one serpentine main channel coupled with an inlet and an outlet that allows flow of the liquid sample in serpentine flow pattern, and

wherein the at least one series of boundaries comprise a plurality of filters with apertures that connect two sections of the serpentine main channel thereby allowing lateral flow of the liquid sample between the two sections.

2. The lateral filter array microfluidic device according to claim 1 , wherein a width of the at least one serpentine main channel is greater than a filter size of the filters.

3. The lateral filter array microfluidic device according to claim 1 , wherein the at least one serpentine main channel has a width ranging from 3 um to 1000 um.

4. The lateral filter array microfluidic device according to claim 1 , wherein a filter size of the plurality of filters is from about 0.03 um to about 100 um.

5. The lateral filter array microfluidic device according to claim 1 , wherein at least one boundary of the at least one series of boundaries is formed by one or more filter support structures having a height ranging from 3 um to 100 um.

6. The lateral filter array microfluidic device according to claim 1 , wherein the target isolate is a cell or cell component, extracellular vesicle, exosome, virus, bacterium, or particle.

7. The lateral filter array microfluidic device according to claim 1 , wherein at least one series of boundaries comprises two or more series of boundaries that each defines a separate serpentine main channel.

8. The lateral filter array microfluidic device according to claim 1 , wherein at least one boundary of the at least one series of boundaries is functionalized to comprise a binding molecule having an affinity to the target isolate.

9. The lateral filter array microfluidic device according to claim 8 , wherein the binding molecule comprises an antibody, an aptamer, multiple antibodies, multiple aptamers, or combinations thereof.

10. The lateral filter array microfluidic device according to claim 8 , wherein the binding molecule is attached to one or more of the plurality of filters.

11. The lateral filter array microfluidic device according to claim 8 , wherein the binding molecule is attached adjacent to apertures and on walls of the serpentine main channel of the device.

12. The lateral filter array microfluidic device according to claim 1 , wherein the target isolate is a cell.

13. The lateral filter array microfluidic device according to claim 12 , wherein the cell is a circulating tumor cell (CTC) or other rare cells.

14. The lateral fluid microfluidic device according to claim 1 , wherein the series of boundaries comprises a first boundary comprising filters of a first filter size and a second boundary comprising a filters of a second filter size, wherein the first filter size and second filter size are different.

15. The lateral fluid microfluidic device according to claim 14 , wherein the first boundary is closer to the inlet and the second boundary is closer to the outlet and wherein the first filter size is greater than the second filter size.

16. The lateral fluid microfluidic device according to claim 14 , further comprising a third boundary having filters of a third filter size and a fourth boundary having filters of a fourth filter size, wherein the third filter size is smaller than the second filter size, and the fourth filter size is smaller than the third filter size.

17. The lateral fluid microfluidic device according to claim 1 , further comprising a cover over the boundaries.

18. The lateral fluid microfluidic device according to claim 17 , wherein the cover is a glass slide and the substrate is PDMS.

19. The lateral fluid microfluidic device according to claim 1 , where the device is comprised of thermoplastic material, silicon or glass, adhesive tapes, thin films, or a combination thereof.

20. The lateral fluid microfluidic device according to claim 19 , wherein the thermoplastic material comprises cyclic olefin copolymer (COC), poly(methyl methacrylate) (PMMA), polycarbonate (PC), polystyrene, polyester, polypropylene, polyurethane, acrylonitrile butadiene styrene (ABS), polylactic acid (PLA), and polytetrafluoroethylene (PTFE).

21. A method of capturing a target isolate in a liquid sample comprising applying the liquid sample to an inlet of a lateral flow microfluidic device according to claim 1 ; and

asserting a force to direct flow of the liquid sample along the serpentine main channel and laterally through filters in the series of boundaries, wherein the target isolate is captured at one or more of the filters.

22. The method according to claim 21 , wherein the filters comprise a binding molecule having affinity for the target isolate.

23. The method of any according to claim 22 , wherein the binding molecule is an antibody, aptamer, multiple antibodies, multiple aptamers, and combinations thereof.

24. The method according to claim 21 , wherein the target isolate a particle, virus, bacterium, extracellular vesicle, exosome, cell or cell component.

25. The method of according to claim 24 , wherein the target isolate is a circulating tumor cell.

26. The method according to claim 25 , wherein the cell is a rare cell.

Assignments (2)
CONFIRMATORY LICENSE Recorded Nov 22, 2023
From: UNIVERSITY OF FLORIDA
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 065664/0651 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 1, 2021
From: FAN, ZHONGHUI HUGH; CHEN, KANGFU
To: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
Reel/Frame 056398/0844 →
Continuity (2)
Provisional Application 62720592 · Aug 21, 2018
Related Publication 20210236992A1 · Aug 5, 2021
References Cited (106)
US 5646001A · Terstappen et al. · 1997 [cited by applicant]
US 5726026A · Wilding et al. · 1998 [cited by applicant]
US 6632655B1 · Mehta et al. · 2003 [cited by applicant]
US 9957472B2 · Chung · 2018 [cited by examiner]
US 20030087265A1 · Sauter et al. · 2003 [cited by applicant]
US 20060140871A1 · Sillerud · 2006 [cited by applicant]
US 20060147941A1 · Su · 2006 [cited by applicant]
US 20080020368A1 · Yang et al. · 2008 [cited by applicant]
US 20090298067A1 · Irimia et al. · 2009 [cited by applicant]
US 20100105053A1 · Cho et al. · 2010 [cited by applicant]
US 20100123457A1 · Shinoda · 2010 [cited by applicant]
US 20100323388A1 · Chiu et al. · 2010 [cited by applicant]
US 20110070581A1 · Gupta et al. · 2011 [cited by applicant]
US 20110158901A1 · Santra · 2011 [cited by applicant]
US 20120070833A1 · Wang et al. · 2012 [cited by applicant]
US 20120077246A1 · Hong et al. · 2012 [cited by applicant]
US 20120100521A1 · Soper et al. · 2012 [cited by applicant]
US 20130035630A1 · Chen · 2013 [cited by applicant]
US 20130190212A1 · Handique · 2013 [cited by examiner]
US 20150056614A1 · Mikolajczyk · 2015 [cited by examiner]
US 20160262934A1 · Siegele · 2016 [cited by examiner]
US 20160279637A1 · Sarioglu et al. · 2016 [cited by applicant]
WO 2013044240 · 2013 [cited by applicant]
WO 2020041471A1 · 2020 [cited by applicant]
Adams, Andre' A. et al., “Highly efficient circulating tumor cell isolation from whole blood and label-free enumeration using polymer-based microfluidics with an integrated conductivity sensor,” J. Am. Chem. Soc. vol 13… [cited by applicant]
Allard, W. Jeffery et al., “Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases,” Clinical Cancer Research, vol. 10, pp. 6897-6904, Oc… [cited by applicant]
Arya, Sunil K. et al., “Enrichment, detection and clinical significance of circulating tumor cells,” Lab Chip, 2013, vol. 13, p. 1995-2027. [cited by applicant]
Bruus, Henrik, “Acoustofluidics 1: Governing equations in microfluidics,” Lab on a Chip, 11(22), 3742-3751, 2011. [cited by applicant]
Capretto, Lorenzo et al., “Micromixing within microfluidic devices,” Top Curr. Chem. 2011, vol. 304, pp. 27-68. [cited by applicant]
Carpenter, Ann E. et al., “CellProfiler: image analysis software for identifying and quantifying cell phenotypes,” Genome Biology, 2006, 7:R100, vol. 7, issue 10, 11 pages. [cited by applicant]
Cen, Putao et al., “Circulating tumor cells in the diagnosis and management of pancreatic cancer,” Biochimica et Biophysica Acta, 1826 (2012) 350-356. [cited by applicant]
Chen, Kangfu et al., “Integration of lateral filter arrays with immunoaffinity for circulating-tumor-cell isolation,” Angew. Chem. Int. Ed. 2019, 58, 7606-7610. [cited by applicant]
Chen, Jian et al., “Microfluidic approaches for cancer cell detection, characterization, and separation,” Lab Chip, 2012, 12, 1753-1767. [cited by applicant]
Chen, Li et al., “Aptamer-mediated efficient capture and release of T Lymphocytes on nanostructured surfaces,” Adv. Mater., 2011, 23, 4376-4380. [cited by applicant]
Chen, Weiqiang et al., “Nanoroughened surfaces for efficient capture of circulating tumor cells without using capture antibodies,” AcsNano, vol. 7, No. 1, pp. 566-575, 2013. [cited by applicant]
Chen, Kangfu et al., “Incorporation of lateral microfiltration with immunoaffinity for enhancing the capture efficiency of fare cells,” Scientific Reports, (2010) 10:14210, 12 pages. [cited by applicant]
Chiu, Yun-Yen et al., “Enhancement of microfluidic particle separation using cross-flow filters with hydrodynamic focusing,” Biomicrofluidics 10, 011906 (2016). [cited by applicant]
Dharmasiri, Udara et al., “Microsystems for the capture of low-abundance cells,” Annual Review of Analytical Chemistry, vol. 3, 2010, pp. 409-432. [cited by applicant]
Dharmasiri, Udara et al., “High-throughout selection, enumeration, electrokinetic manipulation, and molecular profiling of low-abundance circulating tumor cells using a microfluidic system,” Anal. Chem. 2011, 83, 2301-2… [cited by applicant]
Dokukin, Maxim E. et al., “Quanitative study of the elastic modulus of loosely attached cells in AFM indentation experiments,” Biophysical Journal, vol. 104, May 2013, pp. 2123-2131. [cited by applicant]
Forbes, Thomas P. et al., “Engineering and analysis of surface interactions in a microfluidic herringbone micromixer,” Lab Chip, 2012, 12, pp. 2634-2637. [cited by applicant]
Gleghorn, Jason P. et al., “Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunpcapture (GEDI) and prostate-specific antibody,” Lab Chip, 2010,… [cited by applicant]
Guo, Junming PhD. et al., “Detecting carcinoma cells in peripheral blood of patients with hepatocellular carcinoma by Immunomagnetic beads and RT-PCR,” J. Clin Gastroenterol, vol. 41, No. 8, Sep. 2007, pp. 783-788. [cited by applicant]
Han, Woojin et al., “Nanoparticle coatings for enhanced capture of flowing cells in microtubes,” AcsNano, vol. 4, No. 1, pp. 174-180. [cited by applicant]
He, Wei et al., “In vivo quanitation of rare circulating tumor cells by multiphoton intravital flow cytometry,” PNAS, Jul. 10, 2007, vol. 104, No. 28, pp. 11760-11765. [cited by applicant]
Helo, Pauliina et al., “Circulating prostate tumor cells detected by reverse transcription-PCR in men with localized or castration-refractory prostate cancer: Concordance with CellSearch assay and association with bone … [cited by applicant]
Hoshino, Kazunori et al., “Microchip-based immunomagnetic detection of circulating tumor cells,” Lab Chip, 2011, 11, 3449-3457. [cited by applicant]
Hu, Shuhuan et al., “Multiparametric biomechanical and biochemical phenotypic profiling of single cancer cells using an elasticity microcytometer,” Small, 2016, 12, No. 17, pp. 2300-2311. [cited by applicant]
Huang, Yu-Fen et al., “Cancer cell targeting using multiple aptamers conjugated on nanorods,” Anal. Chem. 2008, 80, 567-572. [cited by applicant]
Hurst, Sarah J., “Biomedical Nanotechnology: Methods in molecular biology,” Human Press, 2011, pp. 140-150. [cited by applicant]
Issadore, David et al., “Ultrasensitive clinical enumeration of rare calls ex vivo using a u-Hall detector,” Sci. Transl. Med., Jul. 4, 2012; 4(141), 22 pages. [cited by applicant]
Jiao, P.F. et al., “Cancer-targeting multifunctionalized gold nanoparticles in imaging and therapy,” Current Medicinal Chemistry, 2011, 18, 2086-2102. [cited by applicant]
Kang, Joo H. et al., “A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cells,” Lab Chip, 2012, 12, 2175-2181. [cited by applicant]
Karabacak, Nezihi Murat et al., “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat Protoc., Mar. 2014; 9(3): 694-710. [cited by applicant]
Khoja, L. et al., “A pilot study to explore circulating tumor cells in pancreatic cancer as a novel biomarker,” British Journal of Cancer, (2012) 106, 508-516. [cited by applicant]
Kirpotin, Dmitri B. et al., “Antibody Targeting of Long-Circulating Lipidic Nanoparticles Does Not Increase Tumor Localization but Does Increase Internalization in Animal Models,” Cancer Res 2006; 66: (13). Jul. 1, 2006… [cited by applicant]
Kotz, Kenneth T. et al., “Clinical microfluidics for neutrophil genomics and proteomics,” Nature Medicine, vol. 16, No. 9, Sep. 2010, pp. 1042-1048. [cited by applicant]
Kuo, Jason S. et al., “Deformability considerations in filtration of biological cells,” Lab Chip, 2010, 10, 837-842. [cited by applicant]
Lee, Sang-Kwon et al., “Nanowire substrate-based laser scanning cytometry for quantitation of circulating tumor cells,” Nano Lett., Jun. 13, 2012; 12(6): 2697-2704. [cited by applicant]
Lin, Yu-Li et al., “Compression and deformation of soft spherical particles,” Chemical Engineering Science, 63, (2008) 195-203. [cited by applicant]
Lustberg, Maryam et al., “Emerging technologies for CTC detection based on depletion of normal cells,” in Minimal Residual Disease and circulating Tumor Cells in Breast Cancer, recent Results in Cancer Research, eds M. … [cited by applicant]
Maheswann, Shyamala et al., “Detection of mutations in EGFR in circulating lung cancer cells,” The New England Journal of Medicine, 2008, 359, 366-377. [cited by applicant]
Meunier, Anne et al., “Combination of mechanical and molecular filtration for enhanced enrichment of circulating tumor cells,” Anal. Chem., 2016, 88, 8510-8517. [cited by applicant]
Mikolajczyk, Stephen D. et al., “Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood,” Journal of Oncology, vol. 2011, Article ID 252361, 10 pages, 2011. [cited by applicant]
Murlidhar, Vasudha, “A radial flow microfluidic device for ultra-high-throughput affinity-based isolation of circulating tumor cells,” Small, Dec. 10, 2014; 10(23): 4897-4904. [cited by applicant]
Myung, Ja Hye et al., “Dendrimer-Mediated multivalent binding for the enhanced capture if tumor cells,” Angew. Chem. Int. ed., 2011, 50, 11769-11772. [cited by applicant]
Nagrath, Sunitha et al., “Isolation of rare circulating tumor cells in cancer patients by microchip technology,” Nature, Dec. 20, 2007; 450(7173): 1235-1239. [cited by applicant]
O'Donoghue, Meghan B. et al., “Single-molecule atomic force microscopy on live cells compares aptamer and antibody rupture forces,” Anal Bioanal Chem (2012) 402:3205-3209. [cited by applicant]
Ozhumur, Emre et al., “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci Transl Med., Apr. 3, 2013; 5(179), 20 pages. [cited by applicant]
Pantel, K. et al., “Detection, clinical relevance and specific biological properties of disseminating tumor cells,” Nature Reviews Cancer, vol. 8, May 2008, pp. 329-340. [cited by applicant]
Phillips, Joseph A. et al., “Enrichment of cancer cells using aptamers immobilized on a microfluidic channel,” Anal. Chem., 2009, 81, 1033-1039. [cited by applicant]
Rice, AJ et al., “Matrix stiffness induces epithelial-mesenchymal transition and promotes chemoresistance in pancreatic cancer cells,” Oncogenesis (2017) 6, e352, 9 pages. [cited by applicant]
Riethdorf, Sabine et al., “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: A validation study of the cellSearch system,” Clin Cancer Res., 2007:13(3) Feb. 1, 2007, pp.… [cited by applicant]
Saliba, Antoine-Emmanuel et al., “Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays,” PNAS, Aug. 17, 2010, vol. 107, No. 33, pp. 14524-14529. [cited by applicant]
Schiro, Perry G. et al., “Sensitive and High-Throughput Isolation of Rare Cells from Peripheral Blood with Ensemble-Decision Aliquot Ranking,” Angew Chem Int Ed Engl., May 7, 2012; 51(19): 4618-4622. [cited by applicant]
Shangguan, Dihua et al., “Aptamers evolved from live cells as effective molecular probes for cancer study,” PNAS, Aug. 8, 2006, vol. 103, No. 32, pp. 11838-11843. [cited by applicant]
Sheng, Weian et al., “Aptamer-Enabled Efficient Isolation of Cancer Cells from Whole Blood Using Microfluidic Device,” Anal. Chem. 2012, 84, 4199-4206. [cited by applicant]
Sheng, Weian et al., “Multivalent DNA Nanospheres for Enhanced Capture of Cancer Cells in Microfluidic Devices,” AcsNano, vol. 7, No. 8, 7067-7076, 2013. [cited by applicant]
Sheng, Weian et al., “Capture, Release and Culture of Circulating Tumor Cells from Pancreatic Cancer Patients using an Enhanced Mixing Chip,” Lab Chip, Jan. 7, 2014, 14(1): 89-98. [cited by applicant]
Sia, Samual K. et al., “Microfluific devices fabricated in poly(dimethylsiloxane) for biological studies,” Electrophoresis, 2003, 24, 3563-3576. [cited by applicant]
Stott, Shannon L. et al., “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” PNAS, Oct. 26, 2010, vol. 107, No. 43, pp. 18392-18397. [cited by applicant]
Stroock, Abraham D. et al., “Chaotic Mixer for Microchannels,” Science, vol. 295, Jan. 25, 2002, pp. 647-651. [cited by applicant]
Tang, Yadong et al., “Microfluidic device with integrated microfilter of conical-shaped holes for high efficiency and high purity capture of circulating tumor cells,” Scientific Reports, 4:6052, 2014. [cited by applicant]
Tang, Zhiwen et al., “Selection of Aptamers for Molecular Recognition and Characterization of Cancer Cells,” Anal. Chem., 2007, 79, 4900-4907. [cited by applicant]
Tjensvoll, Kjersti et al., “Circulating tumor cells in pancreatic cancer patients: Methods of detection and clinical Implications,” Int. J. Cancer: 134, 1-8, 2014. [cited by applicant]
PCT/US2019/047505, Search Report and Written Opinion, Mailed date Dec. 20, 2019, 9 pages. [cited by applicant]
Valencia, Pedro M. et al., “Microfluidic technologies for accelerating the clinical translation of nanoparticles,” Nat. Nanotechnol., Oct. 2012; 7(10):623-629. [cited by applicant]
Wang, Shutao et al., “Three-Dimentional Nanostructured Substrates towards Efficient Capture of Circulating Tumor Cells,” Angew. Chem. Int. Ed., 2009, 48, 8970-8973. [cited by applicant]
Wang, Shutao et al., “Highly Efficient Capture of Circulating Tumor Cells by Using Nanostructured Silicon Substrates with Integrated Chaotic Micromixers,” Angew. Chem. Int. Ed., 2011, 50, 3084-3088. [cited by applicant]
Xu, Lei et al., “Optimization and Evaluation of a Novel Size Based Circulating Tumor Cell Isolation System,” PLOS ONE, Sep. 23, 2015, 23 pages. [cited by applicant]
Xu, Ye et al., “Aptamer-Based Microfluidic Device for Enrichment, Sorting, and Detection of Multiple Cancer Cells,” Anal. Chem., 2009, 81, 7436-7442. [cited by applicant]
Yamamura, Shohei et al., “Accurate Detection of Carcinoma Cells by use of a Cell Microarray Chip,” PLOS ONE, Mach 2012, vol. 7, issue 3, 9 pages. [cited by applicant]
Yoon, Yousang et al., “Clogging-free microfluidics for continuous size-based separation of microparticles,” Scientific Reports, Sci. Rep., 6, 26531, 8 pages. [cited by applicant]
Yu, Min et al., “Circulating tumor cells: approaches to isolation and characterization,” The Journal of Cell Biology, 2011, pp. 373-382, vol. 192, No. 3. [cited by applicant]
Zhang, Weijia et al., “Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells,” PNAS, Nov. 13, 2012, vol. 109, No. 46, pp. 18707-18712. [cited by applicant]
Zhao, Weian et al., “Bioinspired multivalent DNA network for capture and release of cells,” PNAS, Nov. 27, 2012, vol. 109, No. 48, pp. 19626-19631. [cited by applicant]
Zhao, Mengxia et al., “An Automated High-Throughput Counting Method for Screening Circulating Tumor Cells in Peripheral Blood,” Anal. Chem., 2013, 85, 2465-2471. [cited by applicant]
Zheng, Xiangjun et al., “A high-performance microsystem for isolating circulating tumor cells,” Lab Chip, 2011, 11, 3269-3276. [cited by applicant]
Gashaw, Metages et al., “Isolation, Detection, and Antigen-Based Profiling of Circulating Tumor Cells Using a Size-Dictated Immunocapture Chip,” Angew. Chem. Int. Ed., 2017, 56, 10681-10685. [cited by applicant]
McFaul, Sarah M. et al., “Cell separation based on size and deformability using microfluidic funnel ratchets,” Lab Chip, 2012, 12, 2369-2376. [cited by applicant]
Moon, Hui-Sung et al., “Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation ( MOFF) and dielectrophoresis (DEP),” Lab Chip, 2011, 11, 1118-1125. [cited by applicant]
Thege, Fredrik I. et al., “Microfluidic immunocapture of circulating pancreatic cells using parrallel EpCAM and MUC1 capture: characterization, optimization and downstream ananlysis,” Lab Chip, 2014, 14, 1775-1784. [cited by applicant]
Zhang, Nangang et al., “Electrospun TiO2 Nanofiber-Based Cell Capture Assay for Detecting Circulating Tumor Cells from Colorectal and Gastric Cancer Patients, ” Adv. Mater., 2012, 24, 2756-2760. [cited by applicant]
Saliba, A-E. et al., “Microfluidic sorting and multimodal typing of cancer cells in self-assembled magnetic arrays,” Proceedings of the National Academy of Sciences of the United States of America, Aug. 17, 2010, pp. 14… [cited by applicant]
Wang, S. et al., “Nano “Fly Paper” Technology for the Capture of Circulating Tumor Cells” Methods in Molecular Biology, 2011, pp. 141-150, vol. 726, Cpt. 10. [cited by applicant]
Written Opinion in International Application No. PCT/US2014/066590, Feb. 19, 2015, pp. 1-9. [cited by applicant]