PESTICIDAL COMBINATIONS OF YERSINIA AND BACILLUS
Disclosed herein are pesticicial combinations, including combinations of Yersinia entomophaga (and/or toxins therefrom) and/or Yersinia nurmii (and/or toxins therefrom) with Bacillus thuringiensis (and/or toxins therefrom). As shown herein, such combinations may provide unexpected pesticidal effects and may be useful for treating insects and other pests and for enhancing plant growth and/or yield.
1 - 63 . (canceled)
64 . A method comprising:
applying a Bacillus thuringiensis pesticide to a plant or plant part in an amount effective to produce a first anticipated insecticidal effect; and
applying a Yersinia entomophaga pesticide to said plant or plant part in an amount effective to produce a second anticipated insecticidal effect,
wherein said Bacillus thuringiensis pesticide comprises one or more Bacillus thuringiensis strains, one or more cell-free Bacillus thuringiensis culture filtrates and/or one or more isolated Bacillus thuringiensis toxins,
wherein said Yersinia entomophaga pesticide comprises one or more Yersinia entomophaga strains, one or more Yersinia entomophaga cell-free culture filtrates and/or one or more isolated Yersinia entomophaga toxins, and
wherein application of said Bacillus thuringiensis pesticide and said Yersinia entomophaga pesticide to said plant or plant part produces an unexpected cumulative insecticidal effect relative to said first anticipated insecticidal effect and said second anticipated insecticidal effect.
65 . The method of claim 64 , wherein said Yersinia entomophaga pesticide comprises at least one Yersinia entomophaga strain having a whole genome sequence that is at least 99.5% identical to the whole genome sequence of Yersinia entomophaga MH96.
66 . The method of claim 64 , wherein said Yersinia entomophaga pesticide comprises Yersinia entomophaga MH96, Yersinia entomophaga NRRL B-67598, Yersinia entomophaga NRRL B-67599, Yersinia entomophaga NRRL B-67600 and/or Yersinia entomophaga NRRL B-67601.
67 . The method of claim 64 , wherein said Yersinia entomophaga pesticide comprises a cell-free filtrate of a culture comprising at least one Yersinia entomophaga strain having a whole genome sequence that is at least 99.5% identical to the whole genome sequence of Yersinia entomophaga MH96.
68 . The method of claim 64 , wherein said Yersinia entomophaga pesticide comprises a cell-free filtrate of a culture comprising Yersinia entomophaga MH96, Yersinia entomophaga NRRL B-67598, Yersinia entomophaga NRRL B-67599, Yersinia entomophaga NRRL B-67600 and/or Yersinia entomophaga NRRL B-67601.
69 . The method of claim 64 , wherein said Yersinia entomophaga pesticide comprises one or more toxins derived from a Yersinia entomophaga strain having a whole genome sequence that is at least 99.5% identical to the whole genome sequence of Yersinia entomophaga MH96.
70 . The method of claim 64 , wherein said Yersinia entomophaga pesticide comprises one or more toxins derived from Yersinia entomophaga MH96, Yersinia entomophaga NRRL B-67598, Yersinia entomophaga NRRL B-67599, Yersinia entomophaga NRRL B-67600 and/or Yersinia entomophaga NRRL B-67601.
71 . The method of claim 64 , wherein said unexpected cumulative insecticidal effect comprises an unexpected magnitude of insecticidal activity against an insect.
72 . The method of claim 64 , wherein said first anticipated insecticidal effect comprises a first anticipated percent mortality of an insect one day after treatment,
wherein said second anticipated insecticidal effect comprises a second anticipated percent mortality of said insect one day after treatment, and
wherein said unexpected cumulative insecticidal effect comprises a percent mortality of said insect one day after treatment that exceeds the numerical sum of said first anticipated percent mortality of said insect one day after treatment and said second anticipated percent mortality of said insect one day after treatment.
73 . The method of claim 64 , wherein said first anticipated insecticidal effect comprises a first anticipated percent mortality of an insect two days after treatment,
wherein said second anticipated insecticidal effect comprises a second anticipated percent mortality of said insect two days after treatment, and
wherein said unexpected cumulative insecticidal effect comprises a percent mortality of said insect two days after treatment that exceeds the numerical sum of said first anticipated percent mortality of said insect two days after treatment and said second anticipated percent mortality of said insect two days after treatment.
74 . The method of claim 64 , wherein said first anticipated insecticidal effect comprises a first anticipated percent mortality of an insect three days after treatment,
wherein said second anticipated insecticidal effect comprises a second anticipated percent mortality of said insect three days after treatment, and
wherein said unexpected cumulative insecticidal effect comprises a percent mortality of said insect three days after treatment that exceeds the numerical sum of said first anticipated percent mortality of said insect three days after treatment and said second anticipated percent mortality of said insect three days after treatment.
75 . The method of claim 64 , wherein said first anticipated insecticidal effect comprises a first anticipated percent mortality of an insect five days after treatment,
wherein said second anticipated insecticidal effect comprises a second anticipated percent mortality of said insect five days after treatment, and
wherein said unexpected cumulative insecticidal effect comprises a percent mortality of said insect five days after treatment that exceeds the numerical sum of said first anticipated percent mortality of said insect five days after treatment and said second anticipated percent mortality of said insect five days after treatment.
76 . The method of claim 64 , wherein said first anticipated insecticidal effect comprises a first anticipated percent mortality of an insect seven days after treatment,
wherein said second anticipated insecticidal effect comprises a second anticipated percent mortality of said insect seven days after treatment, and
wherein said unexpected cumulative insecticidal effect comprises a percent mortality of said insect seven days after treatment that exceeds the numerical sum of said first anticipated percent mortality of said insect seven days after treatment and said second anticipated percent mortality of said insect seven days after treatment.
77 . The method of claim 64 , wherein said first anticipated insecticidal effect comprises a first anticipated percent mortality of an insect nine days after treatment,
wherein said second anticipated insecticidal effect comprises a second anticipated percent mortality of said insect nine days after treatment, and
wherein said unexpected cumulative insecticidal effect comprises a percent mortality of said insect nine days after treatment that exceeds the numerical sum of said first anticipated percent mortality of said insect nine days after treatment and said second anticipated percent mortality of said insect nine days after treatment.
79 . The method of claim 71 , wherein said insect is selected from the group consisting of Coleoptera, Diptera, Hymenoptera, Lepidoptera, Orthoptera and Thysanoptera.
80 . The method of claim 64 , wherein said Bacillus thuringiensis pesticide and said Yersinia entomophaga pesticide are applied to said plant or plant part concurrently.
81 . The method of claim 64 , wherein said Bacillus thuringiensis pesticide and said Yersinia entomophaga pesticide are sprayed onto said plant or plant part.
82 . The method of claim 64 , wherein said plant or plant part comprises plant foliage.
83 . A plant or plant part treated according to the method of claim 64 , said Bacillus thuringiensis pesticide and said Yersinia entomophaga pesticide being present on one or more surfaces of said plant or plant part.