Anti-microbial peptides
Antimicrobial peptides and methods of use are provided.
1. A linear peptide comprising the amino acid sequence RRRWWWWX (SEQ ID NO: 62), wherein X is leucine, valine, or alanine, and
wherein said peptide is less than 12 amino acids in length.
2. The peptide of claim 1 , wherein said peptide comprises SEQ ID NO: 56.
3. The peptide of claim 1 , wherein said peptide comprises at least one D-amino acid.
4. The peptide of claim 3 , wherein all of the amino acids are D-amino acids.
5. The peptide of claim 1 ,
wherein said peptide comprises at least one modification at the C-terminus and/or N-terminus selected from the group consisting of amidation and acetylation.
6. A composition comprising the peptide of claim 1 and at least one pharmaceutically acceptable carrier.
7. The composition of claim 6 , further comprising at least one antibiotic.
8. A method for inhibiting a microbial infection in a subject in need thereof, said method comprising administering to said subject the peptide of claim 1 .
9. The method of claim 8 , further comprising the administration of at least one additional antibiotic.
10. The method of claim 8 , wherein said microbial infection is a staphylococcal infection.
11. The method of claim 8 , wherein said microbial infection is a methicillin-resistant Staphylococcus aureus (MRSA) infection.
12. A medical device or implant comprising the peptide of claim 1 covalently attached to the surface of the medical device or implant.
13. The peptide of claim 1 , wherein said peptide comprises SEQ ID NO: 57.
14. The peptide of claim 1 , wherein said peptide is less than 10 amino acids in length.
15. The peptide of claim 1 , wherein said peptide consists of SEQ ID NO: 57.
16. The composition of claim 6 , further comprising a peptide comprising SEQ ID NO: 54.
17. The peptide of claim 1 , wherein said peptide comprises SEQ ID NO: 58.
18. A peptide comprising the amino acid sequence RRRWWWWX (SEQ ID NO: 62), wherein X is leucine, valine, or alanine,
wherein said peptide is less than 12 amino acids in length, and
wherein said peptide comprises at least one modification at the C-terminus and/or N-terminus selected from the group consisting of amidation and acetylation.
19. The peptide of claim 18 , wherein said peptide comprises SEQ ID NO: 56.
20. The peptide of claim 18 , wherein said peptide comprises SEQ ID NO: 57.
21. The peptide of claim 18 , wherein said peptide comprises SEQ ID NO: 58.
22. The peptide of claim 18 , wherein said peptide consists of SEQ ID NO: 57.
23. The peptide of claim 18 , wherein said peptide is less than 10 amino acids in length.
24. A composition comprising the peptide of claim 18 and at least one pharmaceutically acceptable carrier.
25. The composition of claim 24 , further comprising at least one antibiotic.
26. The composition of claim 24 , further comprising a peptide comprising SEQ ID NO: 54.
27. The peptide of claim 18 , wherein said peptide comprises at least one D-amino acid.
28. The peptide of claim 27 , wherein all of the amino acids are D-amino acids.
29. A method for inhibiting a microbial infection in a subject in need thereof, said method comprising administering to said subject the peptide of claim 18 .
30. The method of claim 29 , further comprising the administration of at least one additional antibiotic.
31. The method of claim 29 , wherein said microbial infection is a staphylococcal infection.
32. The method of claim 29 , wherein said microbial infection is a methicillin-resistant Staphylococcus aureus (MRSA) infection.
33. A medical device or implant comprising the peptide of claim 18 covalently attached to the surface of the medical device or implant.