Methods and compositions for bioprotection of potatoes from
The present invention relates to compositions having antimicrobial activity against Streptomyces scabies . Further provided herein are methods of making and using the antimicrobial compositions to protect and treat potatoes from Streptomyces scabies infections.
1. A method of controlling, suppressing and/or preventing infection from Streptomyces scabies in potatoes, comprising the step of:
providing an antimicrobial composition comprising at least one of Bacillus pumilus strain, a culture medium inoculated with Bacillus pumilus , a purified extract of Bacillus pumilus or at least one metabolite of Bacillus pumilus , wherein said antimicrobial composition comprises Micrococcin P1; and
applying an effective amount of said antimicrobial composition to at least one of potatoes tubers, potatoes roots, potatoes stems, potatoes leaves, and potatoes propagules.
2. The method of claim 1 , wherein the Micrococcin P1 is produced by the Bacillus pumilus.
3. The method of claim 1 , wherein the composition further comprises a cell free supernatant of a microorganism mixture comprising Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactococcus lactis, Bacillus amyloliquefaciens, Aspergillus oryzae, Saccharomyces cerevisiae, Candida utilis , and Rhodopseudomonas palustris , or a mixture thereof.
4. The method of claim 1 , wherein said method provides for bioprotection of potatoes against Streptomyces scabies , and wherein said bioprotection is determined by comparing damages of potatoes contacted or not with said antimicrobial composition.
5. The method of claim 1 , wherein said method provides for at least one of: enhancing resistance against Streptomyces scabies , reduction damage caused by Streptomyces scabies , enhancing plant antimicrobial response against Streptomyces scabies , increasing plant antinematocidal activity, and reducing pathological symptoms or lesions resulting from actions of Streptomyces scabies , and increasing tuber yield.
6. The method of claim 1 , wherein said Bacillus pumilus strain comprises a 16S rRNA having at least 95% identity with SEQ ID NO: 4.
7. The method of claim 1 , wherein said antimicrobial composition further comprises at least one strain of Bacillus subtilis , a culture medium inoculated with Bacillus subtilis , a cell-free extract of Bacillus subtilis or at least one metabolite of Bacillus subtilis.
8. The method of claim 1 , wherein said antimicrobial composition further comprises at least one of an herbicide, an insecticide, a fungicide and a nutrient.
9. The method of claim 1 , wherein the purified extract of Bacillus pumilus comprises a cell-free supernatant of Bacillus pumilus.
10. A composition effective in bioprotection of potatoes from Streptomyces scabies , comprising:
an effective amount of Micrococcin P1; and
an agriculturally acceptable carrier,
wherein said composition is formulated for application to at least one of potatoes tubers, potatoes roots, potatoes stems, potatoes leaves and potatoes propagules; and
wherein said Micrococcin P1 is effective in controlling, suppressing and/or preventing infection from Streptomyces scabies.
11. The composition of claim 10 , wherein the Micrococcin P1 is produced by Bacillus pumilus.
12. The composition of claim 10 , further comprising Bacillus subtilus.
13. The composition of claim 10 , further comprising a filtered fraction of a microbial culture comprising Lactobacillus paracasei, Lactobacillus helveticus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactococcus lactis, Bacillus amyololiquefaciens, Aspergillus oryzae, Saccharomyces cerevisiae, Candida utilis , and Rhodopseudomonas palustris , or a mixture thereof.
14. The composition of claim 10 , wherein said Bacillus pumilus strain comprises a 16S rRNA having at least 95% identity with SEQ ID NO: 4.
15. The composition of claim 10 , further comprising at least one strain of Bacillus subtilis , a culture medium inoculated with Bacillus subtilis , a cell-free extract of Bacillus subtilis or at least one metabolite of Bacillus subtilis.
16. A method of protecting potatoes from Streptomyces scabies , comprising the step of:
applying an effective amount of the composition of claim 10 to a soil where potato plants are growing,
wherein the effective amount is sufficient for bioprotection of the potatoes from Streptomyces scabies.