METHOD FOR DETECTING SILICA
The present invention relates to utilization of lanthanide time resolved fluorescence for determining silica concentration. In the method sample comprising silica is admixed with a reagent comprising a lanthanide (III) ion and optionally a chelating agent, silica in the sample is allowed to interact with the reagent comprising the lanthanide (III) ion, followed by exciting the sample and detecting a signal deriving from the lanthanide (III) ion, and determining the concentration of the silica in the sample by using the detected signal.
1 . A method for determining concentration of silica in a sample comprising silica, the method comprising:
optionally diluting and/or purifying the sample;
admixing the sample with a reagent comprising a lanthanide(III) ion;
allowing the silica in the sample to interact with the reagent comprising the lanthanide(III) ion;
exciting the sample at a excitation wavelength and detecting a sample signal deriving from the lanthanide(III) ion at a signal wavelength by using time-resolved fluorescence measurement; and
determining the concentration of the silica in the sample by using the detected sample signal.
2 . The method according to claim 1 , wherein additionally a lanthanide chelating agent or agents is/are admixed with the sample.
3 . The method according to claim 1 , wherein concentration of the silica in a measurement mixture is in a range of 0.1-100 ppm, preferably 0.5-50 ppm, and more preferably 1-30 ppm.
4 . The method according to claim 1 , wherein concentration of the lanthanide(III) ion in a measurement mixture is in a range of 0.1-100 μM, preferably 0.1-50 μM, and more preferably 1-20 μM
5 . The method according to claim 2 , wherein concentration of the lanthanide chelating agent in a measurement mixture is in a range of 0.01-500 ppm, preferably 0.5-50 ppm, and more preferably 0.5-20 ppm.
6 . The method according to claim 2 , wherein the chelating agent comprises at least one or more functional groups capable of chelating lanthanide(III) ions, preferably one or more groups selected from esters, ethers, thiols, hydroxyls, carboxylates, sulfonates, amides, phosphates, phosphonates, amines or any combination thereof.
7 . The method according to claim 2 , wherein the chelating agent contain additionally aromatic group or groups.
8 . The method according to claim 1 , wherein the silica comprises silicic acid or oligomeric silicate soluble in water.
9 . The method according to claim 1 , wherein the lanthanide(III) ion is selected from europium, terbium, samarium or dysprosium ions, preferably europium or terbium ions.
10 . The method according to claim 1 , wherein the reagent comprises lanthanide(III) salt, preferably halogenide or oxyanion, more preferably hydrated halogenides or nitrates, most preferably chloride.
11 . The method according to claim 1 , wherein the sample is purified by using a purification method selected from the group consisting of centrifugation, size exclusion chromatography, cleaning with solid-phase extraction (SPE) cartridges, dialysis techniques, extraction methods for removing hydrocarbons, filtration, microfiltration, ultrafiltration, nanofiltration, membrane centrifugation and any combinations thereof.
12 . The method according to claim 1 , wherein a pH value of the sample is adjusted to a level in a range between pH 3 and pH 8, preferably in a range from pH 5 to pH 8.
13 . Use of the method according to claim 1 for determining concentration of silica in a sample.
14 . The use according to claim 12 , wherein the sample originates from geothermal processes, cooling towers, desalination plants and water treatment process.
15 . A device comprising means for performing the method according to claim 1 for determining concentration of silica in a sample.