Swab sample nucleic acid releaser and use thereof
The present invention discloses a swab sample nucleic acid releaser and use thereof. The sample releaser includes 1-20 mol/L of a metal ion chelating agent, 5-80 mmol/L of a buffer at pH 6-9, 5%-40% (v/v) of a polar organic solvent, 0.2%-10% (v/v) of a surfactant, and 0.1-1 mol/L of a nuclease inhibitor. The sample releaser can integrate three steps of sampling and preservation, inactivation, and nucleic acid extraction into one step, and after sampling, the sample can be directly put into a sampling tube equipped with the sample releaser for storage and transportation; and when in use, the sampling tube is turned upside down and mixed well, and after the sample and the sample releaser are fully mixed, it is let stand for a few minutes at room temperature to release the nucleic acid of the sample.
1. A swab sample nucleic acid releaser, comprising the following components:
(1) 1 mol/L of a metal ion chelating agent;
(2) 40 mmol/L of a buffer at pH 9;
(3) 40% (v/v) of a polar organic solvent;
(4) 1% (v/v) of a surfactant; and
(5) 1 mol/L of a nuclease inhibitor, wherein the metal ion chelating agent is citric acid; the buffer is a sodium phosphate buffer; the polar organic solvent is hexamethylphosphoramide; the surfactant is SDS; and the nuclease inhibitor is 4,4′-carbonylbis(2-(1-naphthoylamino)benzoic acid).
2. A method of swab sample storing and nucleic acid releasing, comprising: putting a swab into a container containing the sample releaser according to claim 1 , turning the container upside down and mixing for 20- 40 seconds at room temperature, letting stand for 10-20 minutes, and turning upside down again for 20-40 seconds, after an instantaneous centrifugation, a supernatant in the container comprises released nucleic acid.
3. A swab sample nucleic acid releasing kit, wherein the kit contains the sample releaser according to claim 1 .