Granted Patent
B2
US 12,383,653 · App. 17/285,767 · Granted Aug 12, 2025
Neutral multidomain peptide hydrogels and uses thereof
Inventors:
Jeffrey Hartgerink (Houston, TX); David Leach (Houston, TX); Tania Lopez-Silva (Houston, TX)
Assignee:
William Marsh Rice University
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Abstract
Provided herein are peptide-based hydrogels, or neutral multidomain peptide hydrogel (NMDP), as well as uses thereof. The uses include encapsulating cells to induce quiescence for long-term storage and administering to a subject to induce collagen deposition and macrophage infiltration. The disclosed hydrogel is useful for the preservation of stem cells, including maintaining their quiescence and differentiation potential.
Claims (7)
1. A composition comprising a plurality of peptides; wherein each peptide of the plurality of peptides consists of a first domain, a second domain, and a third domain; wherein the first and third domain are each X m , wherein m is 5 and X is 3′-hydroxyproline or 4′-hydroxyproline; wherein the first domain is positioned at the N-terminal end of the second domain; wherein the third domain is positioned at the C-terminal end of the second domain; wherein the second domain comprises (SerLeu) 6 ; and wherein the peptides are N-terminally acetylated.
2. The composition of claim 1 , wherein the amino acid sequence of each peptide consists of SEQ ID NO: 5.
3. The composition of claim 1 , wherein the peptides further comprise a biologically active peptide mimic.
4. The composition of claim 3 , wherein the biologically active peptide mimic has a sequence selected from the group consisting of one of SEQ ID NOs: 10-20.
5. The composition of claim 1 , wherein the composition is lyophilized.
6. A nanofiber comprising a plurality of peptides according to claim 1 .
7. A hydrogel comprising a plurality of peptides according to claim 1 .
Assignments (2)
CONFIRMATORY LICENSE
Recorded Jul 26, 2023
From: RICE UNIVERSITY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 064388/0384 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded May 16, 2022
From: HARTGERINK, JEFFREY; LEACH, DAVID; LOPEZ-SILVA, TANIA
To: WILLIAM MARSH RICE UNIVERSITY
Reel/Frame 059922/0428 →
Continuity (2)
Provisional Application
62746391
· Oct 16, 2018
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