Device to be used for capturing extracellular vesicles, and preservation method and transport method for extracellular vesicles
The present invention provides a novel miRNA extraction method and a method for analyzing miRNA extracted by using said miRNA extraction method. According to the present invention, provided is, for example, a method for extracting miRNA from extracellular vesicles in a sample solution, by using a device capable of capturing extracellular vesicles, the miRNA extraction method comprising: an extracellular vesicle capturing step for capturing extracellular vesicles in a sample solution onto a device by bringing the sample solution and the device in contact with each other; and a miRNA extraction step for homogenizing the extracellular vesicles by bringing the device having captured the extracellular vesicles in contact with a homogenization liquid for extracellular vesicles to extract miRNA from the extracellular vesicle into the homogenization liquid.
1. A method of preserving extracellular vesicles, the preserving method comprising:
an extracellular vesicle capture comprising bringing a device and a sample solution into contact with each other, to capture an extracellular vesicle in the sample solution in the device;
a drying comprising removing a liquid of the sample solution which impregnated the device; and
a preserving comprising preserving the device which is dried in the drying step and has captured the extracellular vesicle,
wherein, the device comprises a nanostructure body made using cellulose nanofibers.
2. The preserving method according to claim 1 , wherein the preserving is carried out at room temperature.
3. The method according to claim 1 , wherein the extracellular vesicle is confined in a nanopore in the dried device.
4. The method according to claim 1 , wherein the sample solution is a non-invasive biological sample solution.
5. The method according to claim 4 , wherein the biological sample solution is saliva.
6. A method of transferring an extracellular vesicle, the transfer method comprising:
an extracellular vesicle capture comprising bringing a device and a sample solution into contact with each other, to capture an extracellular vesicle in the sample solution in the device;
a drying comprising removing a liquid of the sample solution which impregnated the device; and
a transfer comprising transferring the device which is dried in the drying and has captured the extracellular vesicle,
wherein, the device comprises a nanostructure body made using cellulose nanofibers.
7. The transfer method according to claim 6 , wherein the transfer is carried out at room temperature.
8. The method according to claim 6 , wherein the extracellular vesicle is confined in a nanopore in the dried device.
9. The method according to claim 6 , wherein the sample solution is a non-invasive biological sample solution.
10. The method according to claim 9 , wherein the biological sample solution is saliva.
11. A method of extracting miRNA from extracellular vesicles, the extraction method comprising:
an extracellular vesicle capture comprising bringing a device and a sample solution into contact with each other, to capture an extracellular vesicle in the sample solution in the device;
a drying comprising removing a liquid of the sample solution which impregnated the device; and
a preserving comprising preserving the device which is dried in the drying and has captured the extracellular vesicle,
an extracting miRNA from the captured extracellular vesicle,
wherein, the device comprises a nanostructure body made using cellulose nanofibers.
12. The method according to claim 11 , wherein the extracellular vesicle is confined in a nanopore in the dried device.
13. The method according to claim 11 , wherein the sample solution is a non-invasive biological sample solution.
14. The method according to claim 13 , wherein the biological sample solution is saliva.