Crosslinked polysaccharide based absorbents for removal of anti-A and/or anti-B antibodies from human plasma and whole blood
The invention concerns polymeric media based on modified natural polysaccharides for removing one or both of Anti-A Antibodies and Anti-B Antibodies from human blood or plasma, the media comprising one or both of (i) a polymeric solid support with a blood group A Antigen ligand attached to the solid support at a ligand loading between 1-5 mg/mL of solid support, and wherein the media is stable under physiological pH conditions, and (ii) a polymeric solid support with a blood group B Antigen ligand attached to the solid support at a ligand loading between 1-5 mg/mL of solid support, and wherein the media is stable under physiological pH conditions.
1. A polymeric media based on modified natural polysaccharides for removing one or both of Anti-A Antibodies and Anti-B Antibodies from human blood or plasma, the media comprising one or both of
(i) a polymeric solid support comprising a blood group A Antigen ligand attached to the solid support at a ligand loading between 1-5 mg/mL of solid support, and wherein the media is stable under physiological pH conditions, and
(ii) a polymeric solid support comprising a blood group B Antigen ligand attached to the solid support at a ligand loading between 1-5 mg/mL of solid support, and wherein the media is stable under physiological pH conditions;
wherein, prior to ligand attachment, each solid support is functionalized with NaIO 4 oxidation followed by secondary crosslinking with appropriate di, tri or polyamines; and
wherein each solid support comprises a beaded form of functional polysaccharide.
2. The polymeric media of claim 1 wherein the blood group A Antigen ligand is a tetrasaccharide.
3. The polymeric media of claim 2 wherein the blood group A Antigen ligand is
4. The polymeric media of claim 1 wherein the blood group B Antigen ligand is a tetrasaccharide.
5. The polymeric media of claim 4 wherein the blood group B Antigen ligand is
6. The polymeric media of claim 1 , comprising both of said blood group A Antigen ligand attached to said solid support and said blood group B Antigen ligand attached to said solid support.
7. The polymeric media of claim 1 , wherein each solid support comprises a polymer comprising at least one of cellulose, dextran, starch, agarose, and chitosan.
8. The polymeric media of claim 1 , wherein said beaded form of functional polysaccharide with a bead size range of 45-1,000 microns and pore size ranging from non-porous to 1-20,000 angstrom pore diameter.
9. The polymeric media of claim 8 , wherein the polysaccharide is functionalized with natural or synthetic compounds selected from peptides, proteins, polysaccharides, lipids and drugs.
10. The polymeric media of claim 1 , wherein solid support, are stabilized toward degradation and made stable under wide range of pH (1-14) of the media with partial crosslinking with a di, tri, tetra or polyfunctional primary or secondary amine.
11. The polymeric media of claim 10 , wherein the trifunctional amine is tris(2-aminoethyl)amine (TREN).
12. The polymeric media of claim 1 , wherein solid support contains partially unreacted aldehyde groups as functional group to be used for coupling of various amino group containing ligands.