Peptidic blocks for nucleic acid delivery
A polypeptide conjugate for use in a method for binding and/or internalization of the polypeptide conjugate to a mammalian cell having a transferrin receptor (TFRC) and/or receptor for advanced glycation end products (RAGE). The polypeptide conjugate may be used in a method for targeting of a drug delivery system or diagnostic delivery system.
1. A therapeutic composition, comprising:
a peptide segment which is SEQ ID NO. 2 and pharmaceutically acceptable salts or esters thereof; and
a cysteine residue at the N-terminus or C-terminus of the peptide segment.
2. The therapeutic composition of claim 1 , wherein the cysteine residue is at the C-terminus of the peptide segment.
3. The therapeutic composition of claim 1 , wherein the cysteine residue is at the N-terminus of the peptide segment.
4. The therapeutic composition of claim 1 , further comprising a nucleic acid.
5. The therapeutic composition of claim 2 , further comprising a nucleic acid.
6. The therapeutic composition of claim 3 , further comprising a nucleic acid.
7. The therapeutic composition of claim 4 , wherein the nucleic acid is in the form of one of: mRNA, circular RNA, catalytic RNA, RNA decoys, linear DNA, plasmid DNA, a sequence of nucleic acid encoding a desired gene, nucleic acid attached to an enzyme, therapeutic nucleic acid and an expression conjugate that comprises a nucleic acid that encodes a therapeutic protein, an enzyme attached to a clustered regularly interspaced short palindromic repeats, shRNA, miRNA, siRNA, circular siRNA, an anti-sense molecule, locked nucleic acids, aptamer, peptide nucleic acids, splice modulating oligonucleotide, LNA/DNA, and LNA/RNA mix-mer oligonucleotides.
8. The therapeutic composition of claim 7 , wherein the nucleic acid is deoxyribonucleic acid.
9. The therapeutic composition of claim 5 , wherein the nucleic acid is deoxyribonucleic acid.
10. The therapeutic composition of claim 6 , wherein the nucleic acid is deoxyribonucleic acid.
11. The therapeutic composition of claim 5 , wherein the nucleic acid is ribonucleic acid.
12. The therapeutic composition of claim 6 , wherein the nucleic acid is ribonucleic acid.
13. The therapeutic composition of claim 7 , wherein the nucleic acid is ribonucleic acid.