Plant fat-based scaffolds for the growth of cell-based meats and methods of making such products
A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.
1 . A plant fat-based strand scaffold comprising:
a. at least one plant-based saturated fatty acid or wax; and
b. a cell binding protein that enables adherence of non-human animal cells to the plant fat-based strand scaffold for growth;
wherein the at least one plant-based saturated fatty acid or wax is heated to a liquid state at a sterilization temperature; and
wherein the plant fat-based strand scaffold forms by injecting the at least one plant-based saturated fatty acid or wax in the liquid state into a bioreactor at an incorporation temperature that is cooler than the sterilization temperature.
2 . The plant fat-based strand scaffold in claim 1 , wherein the plant fat-based strand scaffold is edible.
3 . The plant fat-based strand scaffold of claim 1 , further comprising one or more secondary components chosen from nutrients and minerals to promote cellular growth, preservatives, coloring agents, flavor increasers, cell binding assistance molecules, and structural support components.
4 . The plant fat-based strand scaffold of claim 1 , wherein the non-human animal cells comprise at least one of myoblasts, myocytes, skeletal muscle progenitor cells, side population cells, mesoangioblasts, mesenchymal stem cells, fibroblasts, pericytes, adipocytes, or pluripotent stem cells.
5 . A method for forming a plant fat-based strand scaffold, said method comprising:
a. providing at least one plant-based saturated fatty acid or wax to form the plant fat-based strand scaffold;
b. heating the at least one plant-based saturated fatty acid or wax to a liquid state at a sterilization temperature;
c. injecting the at least one plant-based saturated fatty acid or wax in the liquid state into a bioreactor at an incorporation temperature that is lower than the sterilization temperature to form a solid plant fat-based strand scaffold;
d. seeding the solid plant fat-based strand scaffold with a population of non-human animal cells for growth; and
e. harvesting at least a portion of the solid plant fat-based strand scaffold and the cells of the population of non-human animal cells after cell growth is complete or after non-human animal tissue is formed.
6 . The method of claim 5 , wherein the portion of the solid plant fat-based strand scaffold can be harvested by melting the solid plant fat-based strand scaffold back into the liquid state and removing the liquid plant fat-based strand scaffold from the population of non-human animal cells.
7 . The method of claim 6 , wherein after harvesting at least the portion of the solid plant fat-based strand scaffold from the population of non-human animal cells, the liquid plant fat-based strand scaffold is recycled as raw material for a formation of another plant fat-based strand scaffold.
8 . The method of claim 5 , wherein the population of non-human animal cells comprise at least one of myoblasts, myocytes, skeletal muscle progenitor cells, side population cells, mesoangioblasts, mesenchymal stem cells, fibroblasts, pericytes, adipocytes, or pluripotent stem cells.
9 . A method for growing a non-human animal tissue product using a plant fat-based strand scaffold, said method comprising:
a. heating at least one plant-based saturated fatty acid or wax to a liquid state at a sterilization temperature;
b. forming the plant fat-based strand scaffold by injecting the at least one plant-based saturated fatty acid or wax in the liquid state into a bioreactor at an incorporation temperature that is cooler than the sterilization temperature;
c. seeding the plant fat-based strand scaffold with a population of non-human animal cells;
d. supplying the population of non-human animal cells with one or more growth nutrients;
e. growing the population of non-human animal cells; and
f. harvesting at least a portion of the plant fat-based strand scaffold and cells of the population of non-human animal cells after cell growth.
10 . The method of claim 9 , wherein the population of non-human animal cells may be supplied with one or more growth nutrients to expedite growth.
11 . The method of claim 10 , wherein the one or more growth nutrients is incorporated into the plant fat-based strand scaffold.
12 . The method of claim 11 , wherein the plant fat-based strand scaffold comprising the one or more growth nutrients has a melting temperature below an upper limit of a growth temperature range of the seeded population of non-human animal cells and below an upper end of an endurance range of the seeded population of non-human animal cells, and by raising a temperature to the melting temperature during a growth phase, the plant fat-based strand scaffold at least partially melts and releases the one or more growth nutrients.
13 . The method of claim 9 , wherein the population of non-human animal cells comprise at least one of myoblasts, myocytes, skeletal muscle progenitor cells, side population cells, mesoangioblasts, mesenchymal stem cells, fibroblasts, pericytes, adipocytes, or pluripotent stem cells.
14 . The method of claim 12 , wherein the endurance range of the population of non-human animal cells comprises between 10 degrees Celsius and 55 degrees Celsius.
15 . A method for growing non-human animal tissue product with a plant fat-based strand scaffold, said method comprising:
a. introducing a plant fat-based strand scaffolding comprising at least one plant-based saturated fatty acid or wax;
b. heating the at least one plant-based saturated fatty acid or wax to a liquid state at a sterilization temperature;
c. seeding the plant fat-based strand scaffold with a population of non-human animal cells while the plant fat-based strand scaffold is in a partially solid and partially liquid state; and
d. cooling the plant fat-based strand scaffold into a solid plant fat-based strand scaffold after the population of non-human animal cells are seeded by injecting the plant fat-based strand scaffold in the partially solid and partially liquid state into a bioreactor at an incorporation temperature that is cooler than the sterilization temperature.
16 . The method of claim 15 , wherein the plant fat-based strand scaffold comprises two or more different types of fats or waxes, each having different melting temperatures.
17 . The method of claim 16 , wherein the plant fat-based strand scaffold is seeded while at least one fat or wax is in the liquid state and at least one fat or wax is in a solid state.
18 . The method of claim 15 , wherein the incorporation temperature matches a growing temperature of the population of non-human animal cells.
19 . The method of claim 15 , further comprising: introducing one or more cell binding proteins while the plant fat-based strand scaffold is partially solid and partially liquid.
20 . The method of claim 15 , wherein the population of non-human animal cells comprise at least one of myoblasts, myocytes, skeletal muscle progenitor cells, side population cells, mesoangioblasts, mesenchymal stem cells, fibroblasts, pericytes, adipocytes, or pluripotent stem cells.