Spectroscopic measurements with parallel array detector
A measurement apparatus comprises optical components arranged to provide parallel measurements of a biological sample. The parallel sample measurements provide improved accuracy with lower detection limit thresholds. The parallel measurements may comprise one or more of Raman spectroscopy measurements or infrared spectroscopy measurements. The parallel measurements can be combined with a light source. In many embodiments, the light source comprises one or more wavelengths corresponding to resonance frequencies of one or more molecules of the sample, such as wavelengths of ultraviolet light. The wavelengths of light corresponding to resonance frequencies can provide an increased signal to noise ratio. The parallel array optical configuration can be combined with wavelengths of light corresponding to resonance frequencies in order to provide increased measurement accuracy and detection of metabolites.
1. An apparatus to measure a blood sample, the apparatus comprising:
a detector to measure a plurality of spatially and temporally resolved spectra of the blood sample;
a light source to illuminate the detector with a light beam;
a support to receive the blood sample and separate the blood sample; and
a processor comprising a tangible medium, the processor coupled to the detector and the light source, the processor configured with instructions to illuminate the blood sample with a measurement beam and measure the plurality of spatially and temporally resolved spectra with the detector while the blood sample separates and to identify a component of the blood sample from the plurality of spatially and temporally resolved spectra.
2. The apparatus as in claim 1 , wherein the light source comprises infrared light.
3. The apparatus as in claim 1 , wherein the blood sample comprises one or more of an individual blood cell, a fluid of the blood sample, a hematocrit of the blood sample, or fluids of the hematocrit and the processor comprises instructions to measure the blood sample.
4. The apparatus as in claim 1 , wherein the plurality of spectra comprises one or more of a plurality of Raman spectra or a plurality of infrared absorbance spectra.
5. The apparatus as in claim 1 , wherein the detector comprises an area array detector.
6. The apparatus as in claim 5 , further comprising:
a plurality of optical filters located on a movable structure coupled to the processor to selectively position each of the plurality of optical filters along an optical path between the area array detector and the support, said each of the plurality of optical filters configured to transmit a range of wavelengths;
wherein the light source comprises a tunable laser configured to emit a laser beam toward the detector; and
wherein the processor comprises instructions to measure an amount of light energy emitted from the blood sample for said each of the plurality of optical filters.
7. The apparatus as in claim 1 , wherein the support comprises an optically transmissive structure to analyze the blood sample.
8. The apparatus of claim 7 , wherein the support is configured to measure transmission of the light beam through the blood sample.
9. The apparatus of claim 7 , wherein the optically transmissive structure is configured to measure the blood sample with an internal reflection mode of the optically transmissive structure.
10. The apparatus as in claim 9 , wherein the blood sample comprises blood cells located on a surface of the optically transmissive structure.