Granted Patent
B2
US 12,280,090 · App. 17/312,806 · Granted Apr 22, 2025
Antimicrobial peptides
Inventors:
Inanc Birol (Vancouver, CA); Caren C. Helbing (Victoria, CA); Stewart Austin Hammond (Saskatoon, CA)
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Abstract
Antimicrobial peptides (AMPs), small compounds that often exhibit broad spectrum antimicrobial activity, are garnering interest as potential therapeutics against antibiotic-resistant bacterial pathogens. Development of new AMPs is arduous due to the practical limitations of classical protein-based discovery approaches. A high throughput bioinformatics approach is described which is able to confirm identification of known AMPs from the North American bullfrog ( Rana ( Lithobates ) catesbeiana ) genome, and a bioinformatics approach is used to develop new AMPs. The described AMPs exhibit antimicrobial activity against Mycobacterium smegmatis via microtitre broth dilution assays, indicating broader efficacy.
Claims (16)
1. An antimicrobial peptide consisting of the amino acid sequence according to SEQ ID NO:46.
2. A composition comprising the antimicrobial peptide according to claim 1 , and a pharmaceutically acceptable excipient.
3. The composition of claim 2 , wherein the composition is formulated for oral, injectable, rectal, topical, transdermal, nasal, or ocular delivery.
4. The composition of claim 2 , wherein the composition is lyophilized.
5. A lipid vesicle comprising the antimicrobial peptide of claim 1 .
6. A nucleic acid molecule encoding the antimicrobial peptide of claim 1 .
7. A vector comprising the nucleic acid molecule of claim 6 .
8. A kit for identifying a target molecule associated with an infectious agent, said kit comprising the antimicrobial peptide of claim 1 together with instructions for conducting a method of identifying a target molecule associated with an infectious agent, wherein said target molecule binds to the antimicrobial peptide,
said method comprising the step of screening a library of candidate target molecules associated with the infectious agent, for a molecule that binds to the antimicrobial peptide;
wherein said infectious agent is Gram-negative bacteria, Gram-positive bacteria, acid fast bacteria, or a drug resistant bacteria.
9. A kit for identifying a target molecule for modulating biological activity, said kit comprising the antimicrobial peptide of claim 1 together with instructions for conducting a method of identifying a target molecule for modulating biological activity, wherein said target molecule binds to the antimicrobial peptide,
said method comprising the step of screening a library of candidate target molecules for a molecule that binds to the antimicrobial peptide.
10. A method of treating bacterial infection in a subject in need thereof, wherein the method comprises administering to the subject a therapeutically effective amount of the antimicrobial peptide according to claim 1 .
11. The method of claim 10 , wherein the bacteria is Gram-negative bacteria.
12. The method of claim 10 , wherein the bacteria is Gram-positive bacteria.
13. The method of claim 10 , wherein the bacteria is Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pyogenes, Mycobacterium smegmatis , Methicillin-resistant Staphylococcus aureus (MRSA), Salmonella enteritidis , or Salmonella Heidelberg.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 6, 2021
From: BIROL, INANC; HAMMOND, STEWART AUSTIN
To: PROVINCIAL HEALTH SERVICES AUTHORITY
Reel/Frame 056760/0468 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 6, 2021
From: HELBING, CAREN C.
To: UVIC INDUSTRY PARTNERSHIPS INC.
Reel/Frame 056760/0905 →
Continuity (2)
Provisional Application
62778450
· Dec 12, 2018
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