Method and apparatus for enhanced photo-thermal imaging and spectroscopy
System for performing chemical spectroscopy on samples from the scale of nanometers to millimeters or more with a multifunctional platform combining analytical and imaging techniques including dual beam photo-thermal spectroscopy with confocal microscopy, Raman spectroscopy, fluorescence detection, various vacuum analytical techniques and/or mass spectrometry. In embodiments described herein, the light beams of a dual-beam system are used for heating and sensing.
1. A method for analyzing a sample, the method comprising:
a, illuminating a region of the sample with a light beam of infrared radiation;
b, illuminating at least a sub-region of the region of the sample with a probe light beam having a shorter wavelength than the light beam of infrared radiation;
c, analyzing probe light collected from the sample to obtain measurements indicative of infrared absorption of the sub-region of the sample; and
d, collecting fluorescent light from the sample in response to stimulation of the sample by the probe light beam.
2. The method of claim 1 wherein a-c are repeated at a plurality of wavelengths of the light beam of infrared radiation.
3. The method of claim 1 further comprising generating a spectrum of infrared absorption by the sub-region of the sample.
4. The method of claim 1 wherein a-d are repeated at a plurality of locations on the sample.
5. The method of claim 4 further comprising generating a map of infrared absorption of the plurality of locations on the sample.
6. The method of claim 5 further comprising using fluorescent light collected from the sample to create a map of fluorescent response of the sample.
7. The method of claim 1 further comprising creating overlapping maps indicative of infrared absorption and fluorescent response of the sample from the measurements indicative of IR absorption and fluorescent light collected from the sample.
8. The method of claim 1 further comprising analyzing probe light to obtain measurements indicative of Raman scattering of the sub-region of the sample.
9. The method of claim 1 wherein the measurement indicative of infrared absorption and the fluorescent light collected from the sample are performed on substantially the same region of the sample.