Self-assembly of protein-based biomaterials with multiple morphologies
The present disclosure relates, in some embodiments, to a composition comprising a biomaterial. A biomaterial may comprise, for example, one or more molecules capable of self-association and/or self-assembly. In some embodiments, a biomaterial may comprise one or more polypeptides and/or proteins. A biomaterial may comprise, for example, two or more self-assembled Ultrabithorax (Ubx) protein molecules. A Ubx protein, in some embodiments, may be any wild type Drosophila melanogaster Ultrabithorax protein, including any natural or non-natural isoforms (e.g., alternative splicing isoforms). The present disclosure relates, in some embodiments, to a method of making a biomaterial comprising contacting two or more Ubx protein molecules under conditions that permit self-assembly to form a first fibril and contacting the first fibril to a second fibril.
1. A method of making a biomaterial comprising a Ubx protein, the method comprising:
a) exposing an aqueous buffer comprising Ubx monomers to air,
b) allowing oligomerization of the Ubx monomers to occur at the air-water interface of the aqueous buffer to form a biomaterial;
c) recovering the biomaterial.
2. The method of claim 1 , wherein the aqueous buffer is a non-denaturing aqueous solution having a pH of about 6 to about 8.
3. The method of claim 1 , wherein oligomerization occurs at a temperature of about 25° C. or lower.
4. The method of claim 1 , wherein the aqueous buffer contains NaCl, sodium phosphate, and glucose.
5. The method of claim 1 , wherein the the biomaterial is a fibril, film, fiber, sheet, bundle, lattice, or encapsulate.
6. The method of claim 1 , wherein the aqueous buffer comprising Ubx monomers is a concentrated Ubx protein solution.
7. The method of claim 1 , wherein oligomerization occurs in less than about 10 hours.
8. The method of claim 7 , wherein oligomerization occurs in about one to about five hours.
9. The method of claim 1 , wherein the aqueous buffer comprising Ubx monomers is placed in a covered chamber.
10. The method of claim 1 , wherein recovery of the biomaterial comprises contacting the air-water interface of the aqueous buffer with a surface, wherein the contacting results in adherence of the biomaterial to the surface.
11. The method of claim 1 , wherein the biomaterial comprises Ubx fibrils having a diameter from about 2 to about 50 μm.