Photobleaching method
The present disclosure provides an improved method for photobleaching an eye of a subject. The disclosed method may be used in a number of psychophysical test methods, including, but not limited to, measurement of dark adaptation. The improved method for photobleaching involves at least one of the following improvements: (i) the use of a bleaching light emitting a particular wavelength of light or a tailored spectrum of wavelengths; (ii) restricting or otherwise spatially tailoring the region of the retina that is subject to photobleaching; and (iii) utilizing a bleaching light having an intensity that is at or below the intensity of ambient daylight. The present disclosure additionally provides a combination of a photobleaching light and an apparatus to administer a psychophysical test suitable for use in practicing the disclosed methods.
1. A method for measuring ability of at least one eye of a test subject to adapt to darkness, the method comprising:
photobleaching one or more photoreceptors of the at least one eye using a photobleaching light having a tailored wavelength spectra spanning within a spectrum visible to human eye;
exposing a retina of the at least one eye to a stimulus light;
obtaining, from the test subject, over a period of time, a series of responses indicating observation of the stimulus light after exposure to the photobleaching light;
administering a dark adaptometry psychophysical test on the at least one eye and determining status of dark adaptation, for the at least one eye, using the series of responses obtained over the period of time; and
measuring the ability of the at least one eye to adapt to darkness using the status of the dark adaptation.
2. The method of claim 1 , wherein the photobleaching light comprises at least one of a wavelength of 650 nm and a wavelength centered at 650 nm.
3. The method of claim 1 , wherein the photobleaching light comprises a wavelength ranging from 600 nm to 700 nm.
4. The method of claim 1 , wherein the photobleaching light comprises a wavelength ranging from 400 nm to 700 nm.
5. The method of claim 1 , wherein the photobleaching light comprises at least one of a wavelength of 460 nm, a wavelength centered at 460 nm, and a wavelength ranging between 440 nm and 460 nm.
6. The method of claim 1 , wherein the photobleaching light comprises at least one of a wavelength of 505 nm, a wavelength centered at 505 nm, a wavelength ranging between 490 nm and 510 nm, and a wavelength ranging between 490 nm and 525 nm.
7. The method of claim 1 , wherein the photobleaching light comprises a wavelength centered at 419 nm.
8. The method of claim 1 , wherein the photobleaching light comprises at least one of a wavelength of 410 nm, a wavelength centered at 410 nm, and a wavelength ranging between 410 nm and 420 nm.
9. The method of claim 1 , wherein the photobleaching light comprises at least one of a wavelength of 570 nm, a wavelength centered at 570 nm, and a wavelength ranging between 560 nm and 580 nm.
10. The method of claim 1 , wherein the photobleaching light comprises at least one of a wavelength of 500 nm, a wavelength centered at 500 nm, and a wavelength ranging between 490 nm and 510 nm.
11. The method of claim 1 , wherein the photobleaching light comprises at least one of a wavelength of 650 nm, a wavelength centered at 650 nm, and a wavelength ranging between 640 nm and 660 nm.
12. The method of claim 1 , wherein the photobleaching light comprises a wavelength centered at 505 nm.
13. The method of claim 1 , wherein the photobleaching light comprises at least one of a wavelength of 560 nm and a wavelength centered at 560 nm.
14. The method of claim 1 , wherein the target stimulus light comprises a wavelength centered at 450 nm.
15. The method of claim 1 , wherein the target stimulus light comprises an intensity of 0 cd/m 2 .
16. The method of claim 1 , further comprising increasing an intensity of the target stimulus light in order to receive a response from the test subject.
17. The method of claim 1 , wherein one or more photoreceptors comprise rod photoreceptors.
18. The method of claim 1 , wherein one or more photoreceptors comprise cone photoreceptors.
19. The method of claim 1 , wherein one or more photoreceptors comprise rod and cone photoreceptors.
20. A system comprising:
a photobleaching light source configured to direct a photobleaching light having a tailored wavelength spectra spanning within a spectrum visible to human eye onto at least one eye of a test subject;
a target stimulus light source configured to direct a target stimulus light onto a retina of the at least one eye; and
an apparatus configured to administer a dark adaptation test to monitor a series of responses from the test subject over a period of time, the responses indicating observation of the target stimulus light after exposure to the photobleaching light, and measure an ability of the at least one eye to adapt to darkness using the series of responses.