IP Library › Granted Patent US 11,773,094
Granted Patent B2
US 11,773,094 · App. 17/349,757 · Granted Oct 3, 2023

Farnesoid X receptor agonists and uses thereof

Inventors: Nicholas D. Smith (San Diego, CA); Steven P. Govek (San Diego, CA); Karensa L. Douglas (San Diego, CA); Andiliy G. Lai (San Diego, CA)
Assignee: ORGANOVO, INC.
C07D471/14C07D413/14C07D417/14C07D487/04
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Quick Facts
Patent No.
US 11,773,094
App. No.
17/349,757
Granted
Oct 3, 2023
Kind
B2
Abstract

Described herein are compounds that are farnesoid X receptor agonists, methods of making such compounds, pharmaceutical compositions and medicaments comprising such compounds, and methods of using such compounds in the treatment of conditions, diseases, or disorders associated with farnesoid X receptor activity.

Claims (67)

1. A method of treating a gastrointestinal disease or condition in a mammal, comprising administering to the mammal a compound of Formula (I′), or a pharmaceutically acceptable salt thereof:

wherein:

ring A is

X 1 is N;

X 5 , X 6 , and X 7 are each CR 7 ;

R 1 is selected from H, halogen, —CN, —OH, —N(R 17 ) 2 , —NR 17 S(═O) 2 (C 1 -C 4 alkyl), —S(═O) 2 N(R 17 ) 2 , —OC(═O)(C 1 -C 4 alkyl), —CO 2 H, —CO 2 (C 1 -C 4 alkyl), —C(═O)N(R 17 ) 2 , —NR 17 C(═O)(C 1 -C 4 alkyl), —NR 17 C(═O)O(C 1 -C 4 alkyl), —OC(═O)N(R 17 ) 2 , —NR 15 C(═O)N(R 17 ) 2 , —SH, —S(C 1 -C 4 alkyl), —S(═O)(C 1 -C 4 alkyl), —S(═O) 2 (C 1 -C 4 alkyl), C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, C 1 -C 4 heteroalkyl, C 3 -C 6 cycloalkyl, and monocyclic C 2 -C 5 heterocycloalkyl;

X 2 is CR 2 ;

R 2 is H, halogen, —CN, —OH, —N(R 17 ) 2 , —NR 17 S(═O) 2 (C 1 -C 4 alkyl), —S(═O) 2 N(R 17 ) 2 , —OC(═O)(C 1 -C 4 alkyl), —CO 2 H, —CO 2 (C 1 -C 4 alkyl), —C(═O)N(R 17 ) 2 , —NR 17 C(═O)(C 1 -C 4 alkyl), —NR 17 C(═O)O(C 1 -C 4 alkyl), —OC(═O)N(R 17 ) 2 , —NR 17 C(═O)N(R 17 ) 2 , —SH, —S(C 1 -C 4 alkyl), —S(═O)(C 1 -C 4 alkyl), —S(═O) 2 (C 1 -C 4 alkyl), C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, C 1 -C 4 heteroalkyl, C 3 -C 6 cycloalkyl, or monocyclic C 2 -C 5 heterocycloalkyl;

X 3 is CR 3 ;

R 3 is H, halogen, —CN, —OH, —N(R 17 ) 2 , —NR 17 S(═O) 2 (C 1 -C 4 alkyl), —OC(═O)(C 1 -C 4 alkyl), —CO 2 H, —CO 2 (C 1 -C 4 alkyl), —C(═O)N(R 17 ) 2 , —NR 17 C(═O)(C 1 -C 4 alkyl), C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, or C 1 -C 4 heteroalkyl;

each X 4 is independently CH or CF;

R 4 and R 5 are taken together to form a bridge that is —CH 2 — or —CH 2 CH 2 —;

each R 6 is independently H, F, —OH, or —CH 3 ;

L is absent;

each R 7 is independently selected from H, halogen, —CN, —OH, C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, C 3 -C 6 cycloalkyl, and C 1 -C 4 heteroalkyl;

R 8 is C 4 -C 8 alkyl or C 4 -C 8 haloalkyl;

R 9 is H, F, or —CH 3 ;

R 10 is —OC(═O)N(R 12 )(R 13 );

R 11 is H F or —CH 3 ·

R 12 and R 13 are taken together to form a 4-, 5-, or 6-membered heterocycloalkyl ring optionally containing an additional heteroatom selected from O, S, and N and optionally substituted with 1, 2, or 3 groups selected from —OH, —N(C 1 -C 4 alkyl) 2 , C 1 -C 6 alkyl, C 1 -C 6 alkoxy, —S(C 1 -C 4 alkyl), —S(═O)(C 1 -C 4 alkyl), —S(═O) 2 (C 1 -C 4 alkyl), —C 1 -C 4 alkyl-S(═O) 2 (C 1 -C 4 alkyl), —C 1 -C 6 alkyl-OR 17 , and —O—C 1 -C 6 alkyl-OR 17 ;

each R 17 is independently H or C 1 -C 6 alkyl;

each R 18 is independently halogen, —CN, —OH, —N(R 17 ) 2 , —NR 17 S(═O) 2 (C 1 -C 4 alkyl), —S(C 1 -C 4 alkyl), —S(═O)(C 1 -C 4 alkyl), —S(═O) 2 (C 1 -C 4 alkyl), —S(═O) 2 N(R 17 ) 2 , —C(═O)(C 1 -C 4 alkyl), —OC(═O)(C 1 -C 4 alkyl), —CO 2 H, —CO 2 (C 1 -C 4 alkyl), —NR 17 C(═O)(C 1 -C 4 alkyl), —C(═O)N(R 17 ) 2 , —NR 17 C(═O)O(C 1 -C 4 alkyl), —OC(═O)N(R 17 ) 2 , C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, C 1 -C 4 heteroalkyl, C 3 -C 6 cycloalkyl, monocyclic C 2 -C 6 heterocycloalkyl, phenyl, or monocyclic heteroaryl;

m is 0, 1, or 2; and

n is 0, 1, or 2.

2. The method of claim 1 , or a pharmaceutically acceptable salt thereof, wherein the compound has the structure of Formula (Ia′), or a pharmaceutically acceptable salt thereof:

3. The method of claim 1 , wherein R 12 and R 13 are taken together to form a 4-, 5-, or 6-membered heterocycloalkyl ring optionally containing an additional heteroatom selected from O, S, and N and optionally substituted with 1, 2, or 3 groups selected from —OH, —N(C 1 -C 4 alkyl) 2 , C 1 -C 6 alkyl, C 1 -C 6 alkoxy, —S(═O) 2 (C 1 -C 4 alkyl), —C 1 -C 4 alkyl-S(═O) 2 (C 1 -C 4 alkyl), —C 1 -C 6 alkyl-OR 17 , and —O—C 1 -C 6 alkyl-OR 17 .

4. The method of claim 3 , wherein R 8 is C 4 -C 8 alkyl.

5. The method of claim 4 , wherein R 4 and R 5 are taken together to form a bridge that is —CH 2 CH 2 —.

6. The method of claim 5 , wherein R 12 and R 13 are taken together to form a 4-membered or 5-membered heterocycloalkyl ring optionally containing an additional heteroatom selected from O, S, and N and optionally substituted with 1 or 2 groups selected from —OH, —N(C 1 -C 4 alkyl) 2 , C 1 -C 6 alkyl, and C 1 -C 6 alkoxy.

7. The method of claim 6 , wherein each X 4 is CH.

8. The method of claim 7 , wherein X 3 is CH.

9. The method of claim 8 , wherein X 2 is CR 2 and R 2 is C 1 -C 4 alkyl.

10. The method of claim 9 , wherein R 1 is C 1 -C 4 alkoxy.

11. The method of claim 10 , wherein m is 0.

12. The method of claim 11 , wherein R 9 is H, R 11 is H, and R 7 is H.

13. A method of treating a gastrointestinal disease or condition in a mammal, comprising administering to the mammal a compound selected from:

or a pharmaceutically acceptable salt thereof.

14. A method of treating primary biliary cirrhosis (PBC), primary sclerosing cholangitis (PSC), nonalcoholic steatohepatitis (NASH), nonalcoholic fatty liver disease (NAFLD), intrahepatic cholestasis, Ala ille syndrome, or biliary atresia in a mammal, comprising administering to the mammal a compound of Formula (I′), or a pharmaceutically acceptable salt thereof:

wherein:

ring A is

X 1 is N;

X 5 , X 6 , and X 7 are each CR 7 ;

R 1 is selected from H, halogen, —CN, —OH, —N(R 17 ) 2 , —NR 17 S(═O) 2 (C 1 -C 4 alkyl), —S(═O) 2 N(R 17 ) 2 , —OC(═O)(C 1 -C 4 alkyl), —CO 2 H, —CO 2 (C 1 -C 4 alkyl), —C(═O)N(R 17 ) 2 , —NR 17 C(═O)(C 1 -C 4 alkyl), —NR 17 C(═O)O(C 1 -C 4 alkyl), —OC(═O)N(R 17 ) 2 , —NR 15 C(═O)N(R 17 ) 2 , —SH, —S(C 1 -C 4 alkyl), —S(═O)(C 1 -C 4 alkyl), —S(═O) 2 (C 1 -C 4 alkyl), C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, C 1 -C 4 heteroalkyl, C 3 -C 6 cycloalkyl, and monocyclic C 2 -C 5 heterocycloalkyl;

X 2 is CR 2 ;

R 2 is H, halogen, —CN, —OH, —N(R 17 ) 2 , —NR 17 S(═O) 2 (C 1 -C 4 alkyl), —S(═O) 2 N(R 17 ) 2 , —OC(═O)(C 1 -C 4 alkyl), —CO 2 H, —CO 2 (C 1 -C 4 alkyl), —C(═O)N(R 17 ) 2 , —NR 17 C(═O)(C 1 -C 4 alkyl), —NR 17 C(═O)O(C 1 -C 4 alkyl), —OC(═O)N(R 17 ) 2 , —NR 17 C(═O)N(R 17 ) 2 , —SH, —S(C 1 -C 4 alkyl), —S(═O)(C 1 -C 4 alkyl), —S(═O) 2 (C 1 -C 4 alkyl), C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, C 1 -C 4 heteroalkyl, C 3 -C 6 cycloalkyl, or monocyclic C 2 -C 5 heterocycloalkyl;

X 3 is CR 3 ;

R 3 is H, halogen, —CN, —OH, —N(R 17 ) 2 , —NR 17 S(═O) 2 (C 1 -C 4 alkyl), —OC(═O)(C 1 -C 4 alkyl), —CO 2 H, —CO 2 (C 1 -C 4 alkyl), —C(═O)N(R 17 ) 2 , —NR 17 C(═O)(C 1 -C 4 alkyl), C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, or C 1 -C 4 heteroalkyl;

each X 4 is independently CH or CF;

R 4 and R 5 are taken together to form a bridge that is —CH 2 — or —CH 2 CH 2 —;

each R 6 is independently H, F, —OH, or —CH 3 ;

L is absent;

each R 7 is independently selected from H, halogen, —CN, —OH, C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, C 3 -C 6 cycloalkyl, and C 1 -C 4 heteroalkyl;

R 8 is C 4 -C 8 alkyl or C 4 -C 8 haloalkyl;

R 9 is H, F, or —CH 3 ;

R 10 is —OC(═O)N(R 12 )(R 13 );

R 11 is H, F, or —CH 3 ;

R 12 and R 13 are taken together to form a 4-, 5-, or 6-membered heterocycloalkyl ring optionally containing an additional heteroatom selected from O, S, and N and optionally substituted with 1, 2, or 3 groups selected from —OH, —N(C 1 -C 4 alkyl) 2 , C 1 -C 6 alkyl, C 1 -C 6 alkoxy, —S(C 1 -C 4 alkyl), —S(═O)(C 1 -C 4 alkyl), —S(═O) 2 (C 1 -C 4 alkyl), —C 1 -C 4 alkyl-S(═O) 2 (C 1 -C 4 alkyl), —C 1 -C 6 alkyl-OR 17 , and —O—C 1 -C 6 alkyl-OR 17 ;

each R 17 is independently H or C 1 -C 6 alkyl;

each R 18 is independently halogen, —CN, —OH, —N(R 17 ) 2 , —NR 17 S(═O) 2 (C 1 -C 4 alkyl), —S(C 1 -C 4 alkyl), —S(═O)(C 1 -C 4 alkyl), —S(═O) 2 (C 1 -C 4 alkyl), —S(═O) 2 N(R 17 ) 2 , —C(═O)(C 1 -C 4 alkyl), —OC(═O)(C 1 -C 4 alkyl), —CO 2 H, —CO 2 (C 1 -C 4 alkyl), —NR 17 C(═O)(C 1 -C 4 alkyl), —C(═O)N(R 17 ) 2 , —NR 17 C(═O)O(C 1 -C 4 alkyl), —OC(═O)N(R 17 ) 2 , C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, C 1 -C 4 alkoxy, C 1 -C 4 fluoroalkyl, C 1 -C 4 fluoroalkoxy, C 1 -C 4 heteroalkyl, C 3 -C 6 cycloalkyl, monocyclic C 2 -C 6 heterocycloalkyl, phenyl, or monocyclic heteroaryl;

m is 0, 1, or 2; and

n is 0, 1, or 2.

15. The method of claim 1 , wherein the gastrointestinal disease or condition is necrotizing enterocolitis, gastritis, ulcerative colitis, Crohn's disease, inflammatory bowel disease, irritable bowel syndrome, gastroenteritis, radiation induced enteritis, pseudomembranous colitis, chemotherapy induced enteritis, gastro-esophageal reflux disease (GERD), peptic ulcer, non-ulcer dyspepsia (NUD), celiac disease, intestinal celiac disease, post-surgical inflammation, gastric carcinogenesis, graft versus host disease, or any combination thereof.

16. The method of claim 15 , wherein the gastrointestinal disease or condition is inflammatory bowel disease or irritable bowel syndrome.

17. The method of claim 13 , wherein the gastrointestinal disease or condition is necrotizing enterocolitis, gastritis, ulcerative colitis, Crohn's disease, inflammatory bowel disease, irritable bowel syndrome, gastroenteritis, radiation induced enteritis, pseudomembranous colitis, chemotherapy induced enteritis, gastro-esophageal reflux disease (GERD), peptic ulcer, non-ulcer dyspepsia (NUD), celiac disease, intestinal celiac disease, post-surgical inflammation, gastric carcinogenesis, graft versus host disease, or any combination thereof.

18. The method of claim 17 , wherein the gastrointestinal disease or condition is inflammatory bowel disease or irritable bowel syndrome.

19. The method of claim 14 , wherein the compound of Formula (I′) is selected from:

or a pharmaceutically acceptable salt thereof.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 21, 2025
From: ORGANOVO, INC.
To: ELI LILLY AND COMPANY
Reel/Frame 070892/0218 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 18, 2023
From: METACRINE, INC.
To: ORGANOVO, INC.
Reel/Frame 063355/0144 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 16, 2021
From: SMITH, NICHOLAS D.; GOVEK, STEVEN P.; DOUGLAS, KARENSA L.; LAI, ANDILIY G.
To: METACRINE, INC.
Reel/Frame 058525/0433 →
Continuity (5)
Continuation 16573993 · Sep 17, 2019
Provisional Application 62881560 · Aug 1, 2019
Provisional Application 62733004 · Sep 18, 2018
Provisional Application 62733006 · Sep 18, 2018
Related Publication 20210395253A1 · Dec 23, 2021
Cited By (1)
US 12,545,660