Three-dimensional printed organs, devices, and matrices
In an aspect, the present disclosure provides a method for printing an object, comprising: directing at least one energy beam from an energy source into a media chamber comprising at least one polymeric precursor to generate said object at a fine resolution within a certain time period.
1. A method for printing an object, comprising: directing at least one energy beam from an energy source into a media chamber comprising at least one polymeric precursor to generate said object at a resolution sufficient to generate a feature with a size of about 20 micrometers (μm) or less and a total volume of said object from about 0.5 to about 2 cubic centimeters (cm 3 ), wherein said object is formed in a time period of at most about 12 hours.
2. The method of claim 1 , wherein said time period is at most about 6 hours.
3. The method of claim 1 , wherein said object comprises at least one cell.
4. The method of claim 3 , wherein said at least one cell is of a subject.
5. The method of claim 3 , wherein said at least one cell is present in said media chamber prior to said directing.
6. The method of claim 3 , wherein said at least one cell is introduced to said object subsequent to generating said object.
7. The method of claim 1 , wherein said directing said at least one energy beam comprises directing a three-dimensional holographic projection of said at least one energy beam into said media chamber.
8. The method of claim 1 , wherein said at least one energy beam comprises a plurality of energy beams.
9. The method of claim 1 , wherein said object corresponds to an organ or organoid selected from the group consisting of a two-dimensional organ or organoid, a three-dimensional organ or organoid, a lymph node, an islet of Langerhans, a hair follicle, a tumor or a tumor spheroid, a neural bundle and support cell(s), a nephron, a liver organoid, an intestinal crypt, a primary lymphoid organ, a secondary lymphoid organ, a spleen, a liver, a pancreas, a gallbladder, an appendix, a small intestine, a large intestine, a heart, a lung, a bladder, a kidney, a bone, a cochlea, an ovary, a thymus, a trachea, a cornea, a heart valve, skin, a ligament, a tendon, a muscle, a thyroid gland, a nerve, and a blood vessel.
10. The method of claim 1 , further comprising receiving computer instructions for printing said object, and forming at least said portion of said object based at least in part on said computer instructions.
11. The method of claim 10 , wherein said computer instructions comprise a computer model of said object.
12. The method of claim 1 , wherein said object comprises a polymeric material, a metal, a metal alloy, a composite material, or any combination thereof.
13. The method of claim 1 , wherein said at least one energy beam is phase modulated.
14. The method of claim 1 , wherein said object comprises signaling molecules or proteins.
15. The method of claim 1 , further comprising, subsequent to (a), forming a second object by directing said at least one energy beam into said media chamber.
16. The method of claim 15 , wherein said second object has a different resolution from said object.
17. The method of claim 15 , wherein said second object is linked to said object.
18. The method of claim 1 , further comprising, subsequent to (a), developing said object into a biologically functional tissue.
19. The method of claim 1 , wherein said energy source is at least one laser source.
20. The method of claim 1 , wherein said energy source is a two-photon energy source.