Virus like nanoparticle compositions and methods thereof
The present disclosure provides virus like nanoparticles (VLPs), which are capable of displaying multiple copies of a SARS-CoV-2 antigen, for eliciting protective immunity against a SARS-CoV-2 infection, as well as polypeptides, compositions and methods thereof.
1. A virus-like nanoparticle (VLP) comprising:
1) a plurality of oligomerized VLP monomer polypeptides, wherein each VLP monomer polypeptide comprises an Fc binding domain amino acid sequence operably linked to a lumazine synthase (LS) amino acid sequence; and
2) a plurality of S-antigen-Fc polypeptides, wherein each S-antigen-Fc polypeptide comprises a SARS-CoV-2 Spike protein domain amino acid sequence operably linked to a Fc domain amino acid sequence.
2. The VLP of claim 1 , wherein the LS amino acid sequence is derived from the LS of Aquifex aeolicus, Brucella spp., Brucella abortus , or Bacillus anthracis.
3. The VLP of claim 1 , wherein the LS amino acid sequence comprises an amino acid sequence having at least about 90% sequence identity to SEQ ID NO:12.
4. The VLP of claim 1 , wherein the Fc binding domain amino acid sequence comprises a Protein A domain sequence.
5. The VLP of claim 4 , wherein the Protein A domain sequence comprises an amino acid sequence having at least about 90% sequence identity to SEQ ID NO:11.
6. The VLP of claim 1 , wherein the VLP monomer polypeptide further comprises one or more linker group(s), an affinity tag and/or a signal peptide sequence.
7. The VLP of claim 1 , wherein the Fc binding domain amino acid sequence is operably linked to the lumazine synthase (LS) amino acid sequence via a linker group.
8. The VLP of claim 1 , wherein the VLP monomer polypeptide comprises an amino acid sequence having at least about 90% sequence identity to SEQ ID NO:15, 16, 17 or 22.
9. The VLP of claim 1 , wherein the SARS-CoV-2 Spike protein domain amino acid sequence comprises a SARS-CoV-2 receptor binding domain (RBD) amino acid sequence or SARS-CoV-2 S1 amino acid sequence.
10. The VLP of claim 1 , wherein the SARS-CoV-2 Spike protein domain amino acid sequence comprises an amino acid sequence having at least about 90% sequence identity to SEQ ID NO:1, or SEQ ID NO:2.
11. The VLP of claim 10 , wherein the SARS-CoV-2 Spike protein domain amino acid sequence comprises SEQ ID NO:1.
12. The VLP of claim 1 , wherein the S-antigen-Fc polypeptide further comprises one or more linker group(s) and/or a signal peptide sequence.
13. The VLP of claim 1 , wherein the SARS-CoV-2 Spike protein domain amino acid sequence is operably linked to the Fc domain amino acid sequence via a linker group.
14. The VLP of claim 1 , wherein the Fc domain amino acid sequence comprises a human IgG1, IgG2, IgG3 or IgG4 Fc domain amino acid sequence.
15. The VLP of claim 1 , wherein the Fc domain amino acid sequence comprises an amino acid sequence having at least about 90% sequence identity to SEQ ID NO:4.
16. The VLP of claim 1 , wherein the S-antigen-Fc polypeptide comprises an amino acid sequence having at least about 90% sequence identity to SEQ ID NO:5, 6, 7, or 21.
17. A pharmaceutical composition comprising a VLP as described in claim 1 and a pharmaceutically acceptable carrier.
18. The composition of 17 , which is a vaccine composition comprising one or more adjuvant(s).
19. A method of eliciting a coronavirus neutralizing antibody response in an animal, comprising administering a first dose of an effective amount of a VLP as described in claim 1 to the animal.
20. The method of claim 19 , wherein the coronavirus is SARS-CoV-1, SARS-CoV-1-like bat coronavirus, or SARS-CoV-2.
21. The method of claim 20 , wherein the coronavirus is SARS-CoV-2.
22. The method of claim 21 , wherein the SARS-CoV-2 is a SARS-CoV-2 variant.
23. The method of claim 19 , further comprising administering to the animal at least one additional agent.
24. The method of claim 23 , wherein the at least one additional agent is an adjuvant, and wherein the adjuvant is selected from the group consisting of monophosphoryl lipid A, aluminum salt, Complete Freund's Adjuvant (CFA), Incomplete Freund's Adjuvant (IFA), MF59, Montanide ISA 51, CpG, Glucopyranosyl Lipid Adjuvant (GLA), QS-21, and combinations thereof.
25. A method of treating a coronavirus infection in an animal, comprising administering a first dose of the composition of claim 17 to the animal.
26. The VLP of claim 1 , wherein the plurality of S-antigen-Fc polypeptides comprises 120 copies of the S-antigen-Fc polypeptide.
27. The VLP of claim 26 , wherein the S-antigen-Fc polypeptide comprises an amino acid sequence having at least about 95% sequence identity to SEQ ID NO:6.
28. The VLP of claim 27 , wherein the VLP monomer polypeptide comprises an amino acid sequence having at least about 95% sequence identity to SEQ ID NO:15.