Universal vaccine platform
The disclosure relates to vaccination compositions, for example, against human papillomavirus, Zika virus, and flu virus. The disclosure also relates to vectors for producing the virus-like particles and immune complex platforms of the vaccination compositions.
1. A recombinant immune complex comprising:
an immunoglobulin heavy chain;
an immunoglobulin light chain;
an epitope tag, wherein the immunoglobulin heavy chain binds the epitope tag; and
a fragment of the virus protein,
wherein the fragment of the virus protein is linked to the N-terminus of the immunoglobulin heavy chain, the epitope tag is linked to the C-terminus of the immunoglobulin heavy chain, and the epitope tag is from a different protein than the virus protein.
2. The recombinant immune complex of claim 1 , wherein the epitope tag is an Ebola antigen.
3. The recombinant immune complex of claim 1 , wherein the immunoglobulin heavy chain is the immunoglobulin heavy chain of humanized 6D8 monoclonal antibody and the epitope tag is the 6D8 epitope tag.
4. The recombinant immune complex of claim 1 , wherein the virus protein is HPV minor capsid protein L2 (GenBank Accession No. AGH32604.1).
5. The recombinant immune complex of claim 4 , wherein the fragment of the virus protein comprises at least 8 continuous amino acids from the first 200 amino acid residues from the N-terminus of HPV minor capsid protein L2.
6. The recombinant immune complex of claim 4 , wherein the fragment of the virus protein comprises at least one peptide sequence from the HPV minor capsid protein L2 selected from the group consisting of: amino acid residues 17-36, amino acid residues 56-75, amino acid residues 65-85, and amino acid residues 96-115.
7. The recombinant immune complex of claim 1 , wherein the virus protein is M2e.
8. The recombinant immune complex of claim 7 , wherein the fragment of the virus protein comprises the amino acid sequence set forth in SEQ ID NO. 9.
9. The recombinant immune complex of claim 7 , wherein the fragment of the virus protein comprises the amino acid sequence set forth in SEQ ID NO. 10.
10. The recombinant immune complex of claim 1 , wherein the virus protein is selected from the group consisting of: zika virus E protein domain 3 protein (ZE3), zika virus fusion loop antigen (ZE), and zika virus soluble envelope protein (Zse).
11. The recombinant immune complex of claim 10 , wherein the fragment of the virus protein comprises amino acid residues 591-696 or amino acid residues 352-412 of GenBank Accession No. AMC13911.1.
12. The recombinant immune complex of claim 10 , wherein the recombinant immune complex is encoded by the plasmid of SEQ ID NO. 38.
13. A method of generating an immune response against a virus in a mammalian subject, the method comprising administering to the mammalian subject a recombinant immune complex (MC), the RIC comprising:
an immunoglobulin heavy chain;
an immunoglobulin light chain;
an epitope tag, wherein the immunoglobulin heavy chain binds the epitope tag; and
a fragment of the virus protein,
wherein the fragment of the virus protein is linked to the N-terminus of the immunoglobulin heavy chain, the epitope tag is linked to the C-terminus of the immunoglobulin heavy chain, and the epitope tag is from a different protein than the virus protein.
14. The method of claim 13 , wherein the epitope tag is an Ebola antigen.
15. The method of claim 13 , wherein the immunoglobulin heavy chain is the immunoglobulin heavy chain of humanized 6D8 monoclonal antibody and the epitope tag is the 6D8 epitope tag.
16. A recombinant immune complex comprising:
an immunoglobulin heavy chain;
an epitope tag, wherein the immunoglobulin heavy chain binds the epitope tag; and
a fragment of the virus protein,
wherein the fragment of the virus protein is linked to the N-terminus of the immunoglobulin heavy chain, the epitope tag is linked to the C-terminus of the immunoglobulin heavy chain, and the epitope tag is from a different protein than the virus protein.
17. The recombinant immune complex of claim 1 , wherein the epitope tag is an Ebola antigen.
18. The recombinant immune complex of claim 1 , wherein the immunoglobulin heavy chain is the immunoglobulin heavy chain of humanized 6D8 monoclonal antibody and the epitope tag is the 6D8 epitope tag.