IP Library › Granted Patent US 12,305,233
Granted Patent B1
US 12,305,233 · App. 17/372,255 · Granted May 20, 2025

Ultra-high-throughput assay for telomerase repeat addition processivity

Inventors: Julian Chen (Chandler, AZ); Yang Li (Chandler, AZ)
Assignee: Arizona Board of Regents on Behalf of Arizona
C12Q1/6876
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Quick Facts
Patent No.
US 12,305,233
App. No.
17/372,255
Granted
May 20, 2025
Kind
B1
Abstract

The present invention provides methods and kits for determining telomerase processivity. These methods and kits may be used to screen for drugs that inhibit or enhance telomerase repeat addition activity, and are readily adapted to an ultra-high-throughput format.

Claims (27)

1. An ultra-high-throughput method for determining telomerase processivity, the method comprising:

a. incubating a telomerase, a telomeric DNA primer, and dNTPs comprising 5-100 μM dGTP under suitable conditions for telomerase activity, optionally in the presence of an agent, thereby forming telomerase-extended DNA products;

b. analyzing the telomerase-extended DNA products to determine the processivity of said telomerase;

c. ligating a hairpin adapter to the telomerase-extended DNA products produced in step (a) prior to step (b) to produce adapter-ligated DNA products; and

d. amplifying the adapter-ligated DNA products to produce a double-stranded DNA amplicon library, wherein step (b) comprises sequencing the double-stranded DNA amplicon library,

wherein;

the telomeric DNA primer comprises:

a part of a telomeric repeat or at least one telomeric repeat at the 3′ end of the telomeric DNA primer; and

a nucleotide barcode, wherein the nucleotide barcode is selected the group consisting of: ACCCTTG, GCCCTTT, GTGTTCG, TGGGTTT, CCCCTTG, GCCGCTT, GTGTTCT, TGGTCCG, CCCTTTT, GCCGTTT, GTGTTGC, TGGTCGT, CCGCTTG, GCCTCTT, GTGTTGT, TGGTCTG, CCGCTTT, GCCTGTT, GTGTTTG, TGGTCTT, CCGGCTT, GCCTTGG, GTGTTTT, TGGTGCT, CCGGTTG, GCCTTGT, GTTCCCT, TGGTGGT, CCGGTTT, GCCTTTG, GTTCCGG, TGGTGTG, CCGTCTT, GCCTTTT, GTTCCTC, TGGTTCG, CCGTTGT, GCGCCTT, GTTCCTG, TGGTTCT, CCGTTTT, GCGCGTT, GTTCGCG, TGGTTGG, CCTCCGT, GCGCTCT, GTTCGCT, TGGTTGT, CCTCCTT, GCGCTTG, GTTCGGT, TGGTTTC, CCTCGGT, GCGCTTT, GTTCGTC, TGGTTTT, CCTCGTG, GCGGCTT, GTTCGTG, TGTCCCG, CCTCTGT, GCGGGTT, GTTCGTT, TGTCCCT, CCTCTTG, GCGGTGT, GTTCTCT, TGTCCGG, CCTGCTT, GCGGTTT, GTTCTGC, TGTCCTG, CCTGTGT, GCGTCTG, GTTCTGT, TGTCCTT, CCTGTTG, GCGTCTT, GTTCTTC, TGTCGCG, CCTGTTT, GCGTGGT, GTTCTTG, TGTCGCT, CCTTCTG, GCGTGTT, GTTCTTT, TGTCGGT, CCTTGGT, GCGTTCG, GTTGCGG, TGTCGTT, CCTTGTG, GCGTTGT, GTTGCGT, TGTCTCG, CCTTTCG, GCGTTTC, GTTGCTC, TGTCTGG, CCTTTCT, GCGTTTG, GTTGCTG, TGTCTGT, CCTTTGG, GCGTTTT, GTTGGTC, TGTCTTG, CCTTTGT, GCTCCGT, GTTGGTG, TGTCTTT, CCTTTTG, GCTCCTT, GTTGGTT, TGTGCCT, CCTTTTT, GCTCGCT, GTTGTCG, TGTGCGG, CGCCCTT, GCTCGGT, GTTGTCT, TGTGCGT, CGCCGTT, GCTCGTC, GTTGTGC, TGTGCTC, CGCCTTG, GCTCGTG, GTTGTGG, TGTGCTG, CGCGCTT, GCTCGTT, GTTGTGT, TGTGCTT, CGCGGTT, GCTCTCG, GTTGTTC, TGTGGTG, CGCGTTG, GCTCTCT, GTTGTTG, TGTGGTT, CGCGTTT, GCTCTGG, GTTGTTT, TGTGTGC, CGCTCGT, GCTCTGT, GTTTCCG, TGTGTGG, CGCTCTT, GCTCTTC, GTTTCGT, TGTGTGT, CGCTTCT, GCTCTTG, GTTTCTC, TGTGTTC, CGCTTGT, GCTCTTT, GTTTCTG, TGTGTTG, CGCTTTG, GCTGCTG, GTTTCTT, TGTTCCG, CGCTTTT, GCTGCTT, GTTTGCG, TGTTCCT, CGGCCTT, GCTGGGT, GTTTGCT, TGTTCGC, CGGCGTT, GCTGGTT, GTTTGGC, TGTTCGT, CGGCTTT, GCTGTGT, GTTTGGG, TGTTCTG, CGGGCTT, GCTGTTT, GTTTGGT, TGTTCTT, CGGGTCT, GCTTCCG, GTTTGTC, TGTTGCG, CGGGTTG, GCTTCCT, GTTTGTG, TGTTGGG, CGGGTTT, GCTTCTT, GTTTTCC, TGTTGGT, CGGTCGT, GCTTGCT, GTTTTCG, TGTTGTC, CGGTCTG, GCTTGGT, GTTTTGC, TGTTGTG, CGGTCTT, GCTTGTC, GTTTTGG, TGTTTCG, CGGTGTG, GCTTGTG, GTTTTGT, TGTTTGG, CGGTGTT, GCTTGTT, GTTTTTG, TGTTTGT, CGGTTCG, GCTTTCG, GTTTTTT, TGTTTTG, CGGTTCT, GCTTTGC, TCCCCGG, TTCCCCG, CGGTTGT, GCTTTGT, TCCCCGT, TTCCCGT, CGGTTTT, GCTTTTC, TCCCCTG, TTCCCTG, CGTCCGT, GCTTTTG, TCCCCTT, TTCCCTT, CGTCCTT, GCTTTTT, TCCCGTT, TTCCGCG, CGTCGCT, GGCCCTT, TCCCTTG, TTCCGGG, CGTCGGT, GGCCGTT, TCCCTTT, TTCCGGT, CGTCGTG, GGCCTTT, TCCGCGT, TTCCGTG, CGTCGTT, GGCGCTT, TCCGCTG, TTCCTCG, CGTCTCG, GGCGGTT, TCCGGTG, TTCCTGT, CGTCTTT, GGCGTTT, TCCGGTT, TTCCTTG, CGTGCGT, GGCTCGT, TCCGTCT, TTCCTTT, CGTGCTT, GGCTCTC, TCCGTGT, TTCGCCG, CGTGGGT, GGCTCTT, TCCGTTG, TTCGCCT, CGTGGTG, GGCTGGT, TCCTCGG, TTCGCTC, CGTGTCT, GGCTGTT, TCCTCGT, TTCGCTG, CGTGTGG, GGCTTCG, TCCTCTT, TTCGCTT, CGTGTGT, GGCTTCT, TCCTGTT, TTCGGCT, CGTGTTG, GGCTTGC, TCCTTGG, TTCGGTG, CGTGTTT, GGCTTGG, TCCTTGT, TTCGGTT, CGTTCCT, GGCTTGT, TCCTTTG, TTCGTCG, CGTTCGT, GGCTTTC, TCCTTTT, TTCGTCT, CGTTCTG, GGCTTTG, TCGCCCT, TTCGTGG, CGTTCTT, GGGCTTT, TCGCCTT, TTCGTGT, CGTTGCG, GGGGTCT, TCGCGCT, TTCGTTC, CGTTGGG, GGGGTTG, TCGCGGT, TTCGTTG, CGTTGGT, GGGGTTT, TCGCGTG, TTCGTTT, CGTTGTG, GGGTCTT, TCGCGTT, TTCTCCG, CGTTGTT, GGGTGTT, TCGCTCG, TTCTCCT, CGTTTCG, GGGTTGT, TCGCTCT, TTCTCGG, CGTTTCT, GGGTTTT, TCGCTTG, TTCTCGT, CGTTTGC, GGTCCCT, TCGCTTT, TTCTCTC, CGTTTGG, GGTCCGT, TCGGCGT, TTCTCTG, CGTTTGT, GGTCCTC, TCGGCTT, TTCTCTT, CGTTTTC, GGTCCTG, TCGGTCT, TTCTGCT, CGTTTTG, GGTCCTT, TCGGTTC, TTCTGGG, CGTTTTT, GGTCGCT, TCGGTTG, TTCTGGT, CTCCCTT, GGTCGTC, TCGGTTT, TTCTGTG, CTCCGCT, GGTCGTT, TCGTCCG, TTCTTGC, CTCCGGT, GGTCTCC, TCGTCGT, TTCTTGG, CTCCGTG, GGTCTCT, TCGTCTG, TTCTTTG, CTCCGTT, GGTCTGT, TCGTCTT, TTCTTTT, CTCCTCG, GGTCTTC, TCGTGGG, TTGCCCG, CTCCTTG, GGTCTTG, TCGTGGT, TTGCCGT, CTCCTTT, GGTCTTT, TCGTGTG, TTGCCTG, CTCGCGT, GGTGCGT, TCGTGTT, TTGCCTT, CTCGCTG, GGTGCTC, TCGTTCG, TTGCGCG, CTCGCTT, GGTGCTT, TCGTTGG, TTGCGCT, CTCGGGT, GGTGGCT, TCGTTGT, TTGCGGG, CTCGTCT, GGTGTCT, TCGTTTG, TTGCGGT, CTCGTGG, GGTGTGT, TCGTTTT, TTGCGTG, CTCGTGT, GGTGTTG, TCTCCCG, TTGCTCT, CTCGTTG, GGTGTTT, TCTCCGG, TTGCTGT, CTCGTTT, GGTTCCG, TCTCCGT, TTGCTTC, CTCTCGG, GGTTCCT, TCTCCTG, TTGCTTG, CTCTCGT, GGTTCGC, TCTCCTT, TTGCTTT, CTCTCTG, GGTTCGG, TCTCGCT, TTGGCCG, CTCTCTT, GGTTCTC, TCTCGGT, TTGGCCT, CTCTTCG, GGTTCTG, TCTCGTC, TTGGCGT, CTCTTCT, GGTTGCT, TCTCGTG, TTGGCTT, CTCTTGG, GGTTGGG, TCTCGTT, TTGGGCT, CTCTTGT, GGTTGTC, TCTCTCT, TTGGGTT, CTCTTTG, GGTTGTG, TCTCTGG, TTGGTGG, CTCTTTT, GGTTGTT, TCTCTGT, TTGGTGT, CTGCCGT, GGTTTCC, TCTCTTG, TTGGTTC, CTGCGGT, GGTTTCG, TCTCTTT, TTGGTTG, CTGCGTG, GGTTTCT, TCTGCCT, TTGGTTT, CTGCGTT, GGTTTGG, TCTGCGT, TTGTCCG, CTGCTCT, GGTTTGT, TCTGCTG, TTGTCCT, CTGCTTG, GGTTTTC, TCTGCTT, TTGTCGT, CTGCTTT, GGTTTTG, TCTGGTG, TTGTCTC, CTGGCGT, GGTTTTT, TCTGGTT, TTGTCTG, CTGGGTG, GTCCCCT, TCTGTCT, TTGTCTT, CTGGGTT, GTCCCGT, TCTGTGG, TTGTGCG, CTGGTCT, GTCCCTT, TCTGTGT, TTGTGCT, CTGGTGT, GTCCGGT, TCTGTTG, TTGTGGG, CTGGTTG, GTCCGTG, TCTGTTT, TTGTGGT, CTGGTTT, GTCCGTT, TCTTCGG, TTGTGTC, CTGTCCG, GTCCTCG, TCTTCTG, TTGTGTG, CTGTCCT, GTCCTCT, TCTTCTT, TTGTGTT, CTGTCGG, GTCCTGT, TCTTGCG, TTGTTCG, CTGTCTG, GTCCTTC, TCTTGCT, TTGTTGC, CTGTCTT, GTCCTTG, TCTTGGG, TTGTTGT, CTGTGCT, GTCCTTT, TCTTGGT, TTGTTTT, CTGTGGT, GTCGCGT, TCTTGTT, TTTCCCG, CTGTGTT, GTCGCTG, TCTTTCG, TTTCCCT, CTGTTCG, GTCGCTT, TCTTTGG, TTTCCGC, CTGTTGG, GTCGGCT, TCTTTGT, TTTCCGG, CTGTTGT, GTCGGTG, TCTTTTG, TTTCCTG, CTGTTTC, GTCGGTT, TGCCCCT, TTTCCTT, CTGTTTG, GTCGTCT, TGCCCGT, TTTCGCG, CTTCCCT, GTCGTGT, TGCCCTT, TTTCGCT, CTTCCGT, GTCGTTG, TGCCGCT, TTTCGGC, CTTCCTT, GTCGTTT, TGCCGTG, TTTCGGG, CTTCGCG, GTCTCCG, TGCCTGT, TTTCGGT, CTTCGGG, GTCTCCT, TGCGCGT, TTTCGTC, CTTCGGT, GTCTCGC, TGCGCTG, TTTCGTT, CTTCGTG, GTCTCGG, TGCGCTT, TTTCTCT, CTTCGTT, GTCTCGT, TGCGGGT, TTTCTGC, CTTCTCG, GTCTCTC, TGCGGTT, TTTCTGG, CTTCTGG, GTCTCTG, TGCGTCT, TTTCTTG, CTTCTGT, GTCTCTT, TGCGTGG, TTTGCCG, CTTCTTC, GTCTGCT, TGCGTGT, TTTGCCT, CTTCTTG, GTCTGGT, TGCGTTG, TTTGCGC, CTTCTTT, GTCTGTT, TGCGTTT, TTTGCGG, CTTGCCT, GTCTTGC, TGCTCCG, TTTGCGT, CTTGCTG, GTCTTGT, TGCTCGC, TTTGCTC, CTTGCTT, GTCTTTC, TGCTCGG, TTTGCTG, CTTGGCT, GTCTTTG, TGCTCGT, TTTGCTT, CTTGGGT, GTCTTTT, TGCTCTG, TTTGGCG, CTTGGTT, GTGCCCT, TGCTCTT, TTTGGGT, CTTGTCG, GTGCCTT, TGCTGCT, TTTGGTG, CTTGTCT, GTGCGCT, TGCTGGT, TTTGGTT, CTTGTGG, GTGCGGT, TGCTGTG, TTTGTCG, CTTGTGT, GTGCGTT, TGCTGTT, TTTGTCT, CTTGTTG, GTGCTCG, TGCTTCG, TTTGTGG, CTTGTTT, GTGCTCT, TGCTTGC, TTTGTGT, CTTTCCG, GTGCTGT, TGCTTGG, TTTGTTG, CTTTCGG, GTGCTTC, TGCTTTC, TTTTCCG, CTTTCGT, GTGCTTG, TGGCCTT, TTTTCCT, CTTTCTC, GTGGGTC, TGGCGCT, TTTTCGC, CTTTCTG, GTGGTCG, TGGCGGT, TTTTCGG, CTTTCTT, GTGGTCT, TGGCGTT, TTTTCGT, CTTTGCG, GTGGTTC, TGGCTCG, TTTTCTG, CTTTGCT, GTGGTTG, TGGCTTC, TTTTCTT, CTTTGGT, GTGTCCG, TGGCTTG, TTTTGCG, CTTTGTG, GTGTCCT, TGGCTTT, TTTTGCT, CTTTGTT, GTGTCGG, TGGGCCT, TTTTGGC, CTTTTCG, GTGTCGT, TGGGCTG, TTTTGGG, CTTTTGC, GTGTCTC, TGGGCTT, TTTTGGT, CTTTTGG, GTGTCTG, TGGGGCT, TTTTGTG, CTTTTGT, GTGTCTT, TGGGGTT, TTTTTCG, CTTTTTG, GTGTGCT, TGGGTCT, TTTTTGC, CTTTTTT, GTGTGGT, TGGGTGT, TTTTTGG, GCCCGTT, GTGTGTC, TGGGTTC, TTTTTGT, GCCCTGT, GTGTGTT, TGGGTTG, and TTTTTTG; and

the hairpin adapter specifically binds to the telomerase-extended DNA products but does not bind to the telomeric DNA primer, and wherein the hairpin adapter comprises:

a sequence that is complementary to the 3′ end of the telomerase-extended DNA products;

a 5′ phosphate to allow for ligation with the telomerase-extended DNA products;

a purification moiety;

a PCR primer binding site;

a stem-loop structure comprising a double-stranded stem and a single-stranded loop; and

a single-stranded 3′-overhang,

wherein the PCR primer binding site is contained within the single-stranded loop, and wherein the sequence that is complementary to the 3′ end of the telomerase-extended DNA products is located within the single-stranded 3′-overhang.

2. The method of claim 1 , wherein the single-stranded 3′-overhang is at least 6 nucleotides in length.

3. The method of claim 1 , wherein the concentration of the hairpin adaptor is at least 10 nM.

4. The method of claim 1 , wherein the method further comprises affinity purifying the telomerase-extended DNA products using the purification moiety of the hairpin adaptor after the telomerase-extended DNA products are ligated to the hairpin adaptor.

5. The method of claim 1 , wherein the nucleotide barcode is flanked by sequences that form a stem, such that the nucleotide barcode resides within a loop region of a stem-loop structure.

6. The method of claim 1 , wherein the incubating in step (a) is performed for 20-30 minutes at room temperature.

7. The method of claim 1 , wherein the method further comprises adding sequencing adapters to the ends of the telomerase-extended DNA products prior to step (b).

8. The method of claim 1 , wherein the telomerase-extended DNA products are pooled from at least 300 reactions and sequenced in the same sequencing reaction.

9. The method of claim 8 , wherein the telomerase-extended DNA products are pooled from at least 30,000 reactions and sequenced in the same sequencing reaction.

10. The method of claim 1 , wherein the sequence that complementary to the 3′ end of telomerase-extended DNA product comprises sequence selected from the group consisting of: CCAATC (3′ to 5′); CAATCC (3′ to 5′); and AATCCC (3′ to 5′).

11. The method of claim 10 , wherein the single-stranded 3′-overhang is 6-7 nucleotides in length.

Assignments (2)
CONFIRMATORY LICENSE Recorded Dec 6, 2023
From: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 065789/0279 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 4, 2021
From: CHEN, JULIAN; LI, YANG
To: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Reel/Frame 058015/0313 →
Continuity (1)
Provisional Application 63050095 · Jul 9, 2020
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