IP Library › Granted Patent US 12,203,106
Granted Patent B2
US 12,203,106 · App. 17/395,800 · Granted Jan 21, 2025

Compositions for increasing polypeptide stability and activity, and related methods

Inventors: Olev Kahre (Tartu, EE); Kadri Artma (Tartumaa, EE); Tiina Kahre (Tartu, EE)
Assignee: Solis BioDyne OÜ
C12N9/1276C07K14/00C12N9/00C12N9/1252C12N9/14C12N9/22C12N9/50C12N9/78C12N9/96C07K2319/21C07K2319/35C07K2319/50C12Y207/07049
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Quick Facts
Patent No.
US 12,203,106
App. No.
17/395,800
Granted
Jan 21, 2025
Kind
B2
Abstract

This disclosure provides peptides, polypeptides, fusion polypeptides, compositions, and methods for enhancing or increasing the stability of a polypeptide (e.g., Taq polymerase). Such peptides, polypeptides, fusion polypeptides, or compositions include polypeptides linked to a peptide tag that enhances the stability of the polypeptide. The peptides, polypeptides, fusion polypeptides, compositions may also enhance the activity, specificity, and/or fidelity of other polypeptides in a reaction mixture. The disclosure also provides methods of using such peptides, polypeptides, fusion polypeptides, compositions.

Claims (6)

1. A fusion polypeptide comprising: (i) a peptide tag that comprises SEQ ID NO: 1 or a conservatively substituted sequence that is at least 85% identical to SEQ ID NO: 1; and (ii) a heterologous polypeptide, wherein the peptide tag increases thermal stability of the heterologous polypeptide, wherein the peptide tag is covalently linked N-terminally to the heterologous polypeptide, and wherein the polypeptide does not have an amino acid sequence as shown in SEQ ID NO: 2.

2. The polypeptide of claim 1 , wherein the peptide tag comprises a protease cleavage site.

3. The polypeptide of claim 1 , wherein the heterologous polypeptide is an enzyme.

4. The polypeptide of claim 3 , wherein the heterologous polypeptide is a polymerase, reverse transcriptase, nuclease, pyrophosphatase, deaminase, or protease.

5. The polypeptide of claim 4 , wherein the enzyme is a polymerase, wherein the polymerase is DNA polymerase I, Thermus aquaticus DNA polymerase I (Taq), Thermococcus gorgonarius DNA polymerase (Tgo), Thermus thermophilics (Tth) DNA polymerase, or ZO5 DNA polymerase.

6. The fusion polypeptide of claim 1 , wherein the peptide tag has a conservatively substituted amino acid sequence that is at least 90% identical to SEQ ID NO: 1.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 18, 2024
From: KAHRE, OLEV; ARTMA, KADRI; KAHRE, TIINA
To: OÜ SOLIS BIODYNE
Reel/Frame 068024/0985 →
CHANGE OF NAME Recorded Jul 18, 2024
From: OÜ SOLIS BIODYNE
To: SOLIS BIODYNE OÜ
Reel/Frame 068435/0817 →
Continuity (9)
Continuation 16570157 · Sep 13, 2019
Continuation 15728344 · Oct 9, 2017
Continuation 15068477 · Mar 11, 2016
Continuation 12950349 · Nov 19, 2010
Provisional Application 61390857 · Oct 7, 2010
Provisional Application 61356541 · Jun 18, 2010
Provisional Application 61350457 · Jun 1, 2010
Provisional Application 61262919 · Nov 19, 2009
Related Publication 20220090034A1 · Mar 24, 2022
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