Granted Patent
B2
US 12,385,077 · App. 17/403,258 · Granted Aug 12, 2025
NAD(P)-dependent responsive enzymes, electrodes and sensors, and methods for making and using the same
Assignee:
Abbott Diabetes Care Inc.
C12Q1/001A61B5/14865C12N11/082C12Q1/004C12Q1/005C12Q1/006C12Q1/26G01N27/3275G01N33/5735G01N33/66C12Y106/05002G01N27/27
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Abstract
NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P) + ) or derivative thereof; an NAD(P) + -dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.
Claims (34)
1. An in vivo analyte sensor comprising:
an electrode having an enzyme composition disposed thereon, the enzyme composition comprising:
nicotinamide adenine dinucleotide (phosphate) (NAD(P) + ) or derivative thereof;
an NAD(P) + -dependent dehydrogenase;
an NAD(P)H oxidoreductase; and
a polymeric redox mediator; and
a membrane disposed on the enzyme composition,
wherein the NAD(P) + or derivative thereof is not covalently bonded to the polymeric redox mediator.
2. The analyte sensor of claim 1 , wherein the NAD(P)H oxidoreductase is bound to the polymeric redox mediator.
3. The analyte sensor of claim 1 , wherein the NAD(P)H oxidoreductase is unbound to the polymeric redox mediator.
4. The analyte sensor of claim 1 , wherein the enzyme composition comprises NAD(P)+.
5. The analyte sensor of claim 1 , wherein the enzyme composition comprises a derivative of NAD(P)+.
6. The analyte sensor of claim 5 , wherein the derivative of NAD(P)+ is a compound of Formula I:
wherein X is alkyl, substituted alkyl, aryl, substituted aryl, acyl, and aminoacyl.
7. The analyte sensor of claim 5 , wherein the derivative of NAD(P)+ is:
8. The analyte sensor of claim 1 , wherein the NAD(P) + -dependent dehydrogenase is D-3-hydroxybutyrate dehydrogenase.
9. The analyte sensor of claim 1 , wherein the NAD(P)H oxidoreductase is diaphorase.
10. The analyte sensor of claim 1 , wherein the polymeric redox mediator comprises an osmium-containing complex.
11. The analyte sensor of claim 10 , wherein the osmium-containing complex comprises three heterocyclic nitrogen-containing bidentate ligands.
12. The analyte sensor of claim 10 , wherein the osmium-containing complex comprises two biimidazole and one imidazole-pyridine bidentate ligands.
13. The analyte sensor of claim 1 , wherein the polymeric redox mediator comprises a heterocycle-containing polymer and wherein the enzyme composition further comprises a crosslinker.
14. The analyte sensor of claim 13 , wherein the heterocycle-containing polymer comprises polyvinyl pyridine.
15. The analyte sensor of claim 1 , wherein:
the NAD(P) + -dependent dehydrogenase is glucose dehydrogenase;
the NAD(P)H oxidoreductase is diaphorase; and
the polymeric redox mediator comprises an osmium-containing complex.
16. The analyte sensor of claim 1 , wherein:
the NAD(P) + -dependent dehydrogenase is alcohol dehydrogenase;
the NAD(P)H oxidoreductase is diaphorase; and
the polymeric redox mediator comprises an osmium-containing complex.
17. The analyte sensor of claim 1 , wherein:
the NAD(P) + -dependent dehydrogenase is D-3-hydroxybutyrate dehydrogenase;
the NAD(P)H oxidoreductase is diaphorase; and
the polymeric redox mediator comprises an osmium-containing complex.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 16, 2021
From: OUYANG, TIANMEI; FELDMAN, BENJAMIN J.
To: ABBOTT DIABETES CARE INC.
Reel/Frame 057191/0503 →
Continuity (3)
Continuation
16081162
Provisional Application
62303626
· Mar 4, 2016
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