Ovarian cancer vaccines
Provided herein are methods, kits and compositions for the treatment and/or prevention of ovarian cancer through the induction of an immune response against Anti-Mullerian Hormone Receptor, Type II (AMHR2).
1. A method of treating an ovarian cancer tumor in a female human subject comprising: administering to the subject an immunogenic composition comprising a nucleic acid encoding an Anti-Mullerian Hormone Receptor, Type II (AMHR2) polypeptide,
wherein the AMHR2 polypeptide comprises the extracellular domain of human AMHR2,
wherein the immunogenic composition elicits an immune response specific for an AMHR2 polypeptide comprising the extracellular domain of human AMHR2.
2. The method of claim 1 , wherein the nucleic acid encoding the AMHR2 polypeptide is an expression vector encoding the AMHR2 polypeptide.
3. The method of claim 1 , wherein the nucleic acid encoding the AMHR2 polypeptide comprises an mRNA encoding the AMHR2 polypeptide.
4. The method of claim 1 , wherein the nucleic acid encoding the AMHR2 polypeptide is operably incorporated into a delivery vector.
5. The method of claim 4 , wherein the delivery vector is selected from biodegradable microcapsules, immune-stimulating complexes, and liposomes.
6. The method of claim 4 , wherein the delivery vector is a viral vector.
7. The method of claim 6 , wherein the viral vector is an adenovirus, an adeno-associated virus, a vaccinia virus, or a baculovirus.
8. The method of claim 1 , wherein the immunogenic composition comprises an adjuvant.
9. The method of claim 8 , wherein the adjuvant is selected from: Adjuvant 65, α-GalCer, aluminum phosphate, aluminum hydroxide, calcium phosphate, β-Glucan Peptide, CpG DNA, GM-CSF, GPI-0100, IFA, IFN-γ, IL-17, lipid A, lipopolysaccharide, Lipovant, Montanide, N-acety 1-muramy 1-L-alany 1-D-isoglutamine, Pam3CSK4, poly-IC, quil A, trehalose dimycolate and zymosan.
10. The method of claim 8 , wherein the adjuvant is one that induces a mixed type I/type 17 immune response.
11. The method of claim 1 , wherein the ovarian cancer tumor is epithelial ovarian cancer (EOC) tumor.
12. The method of claim 1 , wherein the ovarian cancer tumor expresses AMHR2.
13. The method of claim 1 , further comprising a step of determining whether the ovarian cancer tumor expresses AMHR2 and/or wherein the adjuvant is aluminum.
14. A method comprising: vaccinating a female subject with a vaccine comprising an immunogenic composition comprising nucleic acid encoding an Anti-Mullerian Hormone Receptor, Type II (AMHR2) polypeptide,
wherein the AMHR2 polypeptide comprises the extracellular domain of human AMHR2, and
wherein the immunogenic composition elicits an immune response specific for an AMHR2 polypeptide comprising the extracellular domain of AMHR2.
15. The method of claim 14 , wherein the nucleic acid encoding the AMHR2 polypeptide is an expression vector encoding the AMHR2 polypeptide.
16. The method of claim 14 , wherein the nucleic acid encoding the AMHR2 polypeptide comprises an mRNA encoding the AMHR2 polypeptide.
17. The method of claim 14 , wherein the female human subject comprises a BRCA1 or BRCA2 mutation in their genome.
18. The method of claim 14 , wherein the female human subject has a family history of ovarian cancer.
19. The method of claim 14 , wherein the female human subject is a post-menopausal human female.