Paramyxovirus vaccine strain for novel coronavirus pneumonia and construction method thereof
A paramyxovirus vaccine strain for novel coronavirus pneumonia and a construction method thereof are provided. The method includes performing a recombination of N and F genes of Newcastle disease virus type VII of Paramyxoviridae with P, M, H, L genes of Canine distemper virus of Paramyxoviridae to obtain a recombinant virus, inserting S1 gene of the novel coronavirus between the P and M genes of the recombinant virus to obtain a recombinant vector. The vaccine strain constructed can stably and efficiently express the novel coronavirus S1 protein, and induce the body to produce antibodies; and the recombined virus vaccine strain can stimulate the human body to produce mucosal immunity, and the prepared vaccine can be vaccinated through a nasal spray. Moreover, the vaccine strain can be tested in poultry and dogs, saving time, reducing costs, and being more conducive to actual production due to large output.
1. A paramyxovirus vaccine strain for novel coronavirus pneumonia, wherein the paramyxovirus vaccine strain for novel coronavirus pneumonia is a recombinant paramyxovirus strain that expresses the novel coronavirus S1 protein (rCNDV-S), with an access number CCTCC NO: V202119, deposited in China Center for Type Culture Collection, Wuhan University, Wuhan, China.
2. A vaccine composition, comprising a pharmaceutically effective amount of the paramyxovirus vaccine strain for novel coronavirus pneumonia according to claim 1 and a pharmaceutically acceptable adjuvant.
3. The vaccine composition according to claim 2 , wherein the pharmaceutically acceptable adjuvant is one or more selected from the group consisting of propolis, liposomes, lentinan, ganoderma polysaccharide, astragalus polysaccharide, gastrodia extract, cordyceps extract, Sophora flavescens extract, and levamisole.