Systems, devices, and methods for tissue layer removal
The present disclosure provides systems, devices, and methods for penetrating a biological membrane. The system may comprise a laser unit configured to generate one or more laser beams. The system may comprise a set of targeting optics configured to direct the one or more laser beams to a target region of the biological membrane. The system may comprise a raster scanner operatively coupled to the laser unit and the set of targeting optics. The system may comprise a non-transitory computer readable storage medium comprising a set of instructions. The set of instructions may be configured to control at least one of the laser unit, the set of targeting optics, or the raster scanner to photodisrupt the target region of the biological membrane to a target depth while minimizing damage to one or more blood vessels in proximity to the target region.
1 . A method of penetrating a biological membrane in proximity to a subject's brain, comprising:
(a) identifying a target region of the biological membrane;
(b) photodisrupting the target region to a target depth to remove or ablate at least a portion of the biological membrane to expose underlying brain tissue, wherein the biological membrane is a pia mater, wherein the photodisrupting comprises using at least one light source and one or more optical elements, and wherein the at least one light source emits one or more pulses with a pulse energy density from about 1 μJ/cm 2 to about 100 J/cm 2 ; and
(c) inserting an implantable device comprising one or more high aspect ratio structures through the photodisrupted target region into a tissue below the biological membrane, wherein the tissue below the biological membrane comprises the underlying brain tissue, wherein the implantable device is configured to receive or transmit signals to the subject's brain.
2 . The method of claim 1 , further comprising removing one or more particles from (i) the target region or (ii) air surrounding the target region.
3 . The method of claim 2 , further comprising using a vacuum or a blower to remove the one or more particles from (i) the target region or (ii) the air surrounding the target region.
4 . The method of claim 2 , wherein the one or more particles are removed by flowing a gas across the target region.
5 . The method of claim 4 , wherein the gas humidifies one or more tissue regions in the target region to prevent the one or more tissue regions from drying out.
6 . The method of claim 4 , wherein the gas modulates an ablation characteristic or tissue response of one or more tissue regions in the target region.
7 . The method of claim 1 , wherein the one or more optical elements are configured to spatially block or redirect light from the at least one light source to (i) avoid ablating one or more blood vessels in proximity to the target region and (ii) minimize bleeding in or near the target region.
8 . The method of claim 1 , wherein at least a portion of the one or more optical elements are optically aligned with the at least one light source.
9 . The method of claim 1 , wherein the one or more optical elements comprise a mirror, a lens, a light cable, or one or more masks.
10 . The method of claim 1 , wherein the at least one light source is configured to generate a light pattern to avoid ablation of one or more blood vessels in proximity to the target region and to prevent bleeding in or near the target region.
11 . The method of claim 10 , wherein the at least one light source is configured to use image processing to generate the light pattern.
12 . The method of claim 11 , wherein the at least one light source is configured to interrupt laser pulses over the one or more blood vessels as ablation is being performed, based at least in part on one or more sensor readings or measurements.
13 . The method of claim 1 , further comprising using an image recognition module or one or more ranging sensors to actively or dynamically adjust a light pattern or a focus of the at least one light source to maintain uniform power on one or more tissue regions during ablation.
14 . The method of claim 1 , further comprising using a cauterization module to target one or more ablated blood vessels and to adjust a power or a focus of the at least one light source to cauterize the one or more ablated blood vessels post-ablation.
15 . The method of claim 1 , wherein in (b), the photodisrupting of the target region is performed at a predetermined intensity or for a predetermined time interval.
16 . The method of claim 1 , wherein the one or more high aspect ratio structures comprise a microwire, a microelectrode, or a probe.
17 . The method of claim 1 , wherein the at least one light source comprises light having a wavelength of at most 360 nm.
18 . The method of claim 1 , wherein the at least one light source has an average power of at least 0.1 milliwatts.
19 . The method of claim 1 , wherein the at least one light source comprises a pulsed laser, wherein the pulsed laser is configured to generate the one or more pulses, the one or more pulses having (i) a duration of 1 picosecond to 10 microseconds or (ii) a predetermined pulse repetition frequency between 1 hertz (Hz) and 100 megahertz (MHz).
20 . The method of claim 1 , wherein the implantable device is a brain machine interface (BMI) device.
21 . The method of claim 1 , wherein the implantable device is configured to record neural signals.
22 . A method of penetrating a biological membrane in proximity to a subject's brain, comprising:
(a) identifying a target region of the biological membrane;
(b) photodisrupting the target region to a target depth to remove at least a portion of the biological membrane to expose underlying brain tissue, wherein the biological membrane is a pia mater, wherein the photodisrupting comprises using at least one light source and one or more optical elements, and wherein the at least one light source emits one or more pulses with a pulse energy density from about 1 μJ/cm 2 to about 100 J/cm 2 ; and
(c) inserting one or more high aspect ratio structures through the photodisrupted target region into a tissue below the biological membrane, wherein the tissue below the biological membrane comprises the underlying brain tissue.