Filovirus antibodies and methods
An antibody that binds to a filovirus glycoprotein generally includes include a complementarity determining region (CDR) of any one of SEQ ID NO:27-36 or a combination of such CDRs. The antibody may be used in to detect filovirus in a biological sample obtained from a subject. The antibody also may be formulated into a pharmaceutical composition for administering to a subject having, or at risk of having, a filovirus infection.
1. An antibody comprising:
Complementarity Determining Region (CDR) 1 variable heavy chain (V H ) of EbovGPscFv4-2 (SEQ ID NO:30);
CDR2 V H of EbovGPscFv4-2 (SEQ ID NO:30);
CDR3 V H of EbovGPscFv4-2 (SEQ ID NO:30);
CDR1 variable light chain (V L ) of EbovGPscFv4-2 (SEQ ID NO:30);
CDR2 V L of EbovGPscFv4-2 (SEQ ID NO:30); or
CDR3 V L of EbovGPscFv4-2 (SEQ ID NO:30).
2. The antibody of claim 1 , wherein the antibody is an antibody fragment.
3. The antibody of claim 1 , wherein the antibody binds to a filovirus.
4. The antibody of claim 3 , wherein the antibody binds to at least one of: Ebola virus (EBOV) glycoprotein, Sudan ebolavirus (SUDV) glycoprotein, Reston ebolavirus (RESTV) glycoprotein, Bundibugyo ebolavirus (BDBV) glycoprotein, Marburg ebolavirus (MARV) glycoprotein, or Tai Forest ebolavirus (TAFV) glycoprotein.
5. The antibody of claim 3 , wherein the antibody binds to EBOV glycoprotein, SUDV glycoprotein, RESTV glycoprotein, BDBV glycoprotein, MARV glycoprotein, and TAFV glycoprotein.
6. A method of detecting a filovirus in a biological sample obtained from a subject, the method comprising:
obtaining the biological sample from the subject;
contacting the antibody of claim 1 with the biological sample under conditions effective to allow the antibody to bind to filovirus in the sample, thereby forming an antibody: target complex; and
detecting the antibody: target complex.
7. The method of claim 6 , wherein the filovirus is an ebolavirus.
8. The method of claim 6 , wherein the filovirus is a marburgvirus.
9. The method of claim 6 , wherein detecting the antibody: target complex comprises performing an enzyme-linked immunosorbent assay (ELISA).
10. The method of claim 6 , wherein detecting the antibody: target complex comprises performing Western blot analysis.
11. The method of claim 6 , wherein detecting the antibody: target complex comprises performing an immunofluorescence assay.
12. A method of treating a subject having, or at risk of having, a filovirus infection, the method comprising:
administering to the subject an effective amount of a pharmaceutical composition comprising the antibody of claim 1 .
13. The method of claim 12 , wherein the pharmaceutical composition is administered before the subject exhibits a symptom or clinical sign of having a filovirus infection.
14. The method of claim 12 , wherein the pharmaceutical composition is administered after the subject exhibits a symptom or clinical sign of having a filovirus infection.
15. The method of claim 12 , wherein the filovirus is an ebolavirus.
16. The method of claim 12 , wherein the filovirus is a marburgvirus.