IP Library Granted Patent US 12,157,906
Granted Patent B2
US 12,157,906 · App. 17/437,566 · Granted Dec 3, 2024

Process for producing a fermentation broth

Inventors: Herman Jan Pel (Echt, NL); Maaike Appeldoorn (Echt, NL); Rolf Poldermans (Echt, NL)
Assignee: VERSALIS S.P.A.
C12P1/02C12N1/14C12N9/24
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,157,906
App. No.
17/437,566
Granted
Dec 3, 2024
Kind
B2
Abstract

The invention relates to a process for producing a fermentation broth by culturing a microorganism.

Claims (23)

1. A process for producing a mixed whole fermentation broth comprising one or more cellulolytic and/or hemicellulolytic enzymes, wherein the process comprises:

(i) culturing a first filamentous fungus in the presence of an inducer to produce a first whole fermentation broth comprising one or more cellulolytic and/or hemicellulolytic enzymes,

(ii) culturing a second filamentous fungus in the absence of the inducer to produce a second whole fermentation broth comprising one or more cellulolytic and/or hemicellulolytic enzymes, and

(iii) combining the first whole fermentation broth and the second whole fermentation broths to produce the mixed whole fermentation broth comprising one or more cellulolytic and/or hemicellulolytic enzymes,

wherein the first filamentous fungus is different from the second filamentous fungus; and

wherein the inducer is selected from the group consisting of cellulose, carboxymethylcellulose (CMC), xylan, xylose, gentiobiose, sophorose, lactose, glycerol, glucose, sorbitol, cellobiose, xylobiose, galactose, laminaribiose, maltose, fructose, mannitol, arabinose, arabinitolo, mannose, sorbose, and a mixture of disaccharides prepared from glucose by incubating glucose with a transglycosylating beta-glucosidase or any combination thereof.

2. The process according to claim 1 , wherein each of the first filamentous fungus and the second filamentous fungus is selected from the group of genera consisting of Acremonium, Agaricus, Aspergillus, Aureobasidium, Beauvaria, Cephalosporium, Ceriporiopsis, Chaetomium paecilomyces, Chrysosporium, Claviceps, Cochiobolus, Coprinus, Cryptococcus, Cyathus, Emericella, Endothia, Endothia mucor, Filibasidium, Fusarium, Geosmithia, Gilocladium, Humicola, Magnaporthe, Mucor, Myceliophthora, Myrothecium, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Piromyces, Panerochaete, Pleurotus, Podospora, Pyricularia, Rasamsonia, Rhizomucor, Rhizopus, Scylatidium, Schizophyllum, Stagonospora, Talaromyces, Thermoascus, Thermomyces, Thielavia, Tolypocladium, Trametes pleurotus, Trichoderma and Trichophyton.

3. A process for producing a mixed whole fermentation broth comprising one or more cellulolytic and/or hemicellulolytic enzymes, wherein the process comprises:

(i) culturing a first filamentous fungus in the presence of an inducer to produce a first whole fermentation broth comprising the one or more cellulolytic and/or hemicellulolytic enzymes,

(ii) culturing a second filamentous fungus in the absence of the inducer to produce a second whole fermentation broth comprising the one or more cellulolytic and/or hemicellulolytic enzymes, and

(iii) combining the first whole fermentation broth and the second whole fermentation broths to produce the mixed whole fermentation broth comprising the one or more cellulolytic and/or hemicellulolytic enzymes,

wherein, the inducer is selected from the group consisting of cellulose, carboxymethylcellulose (CMC), xylan, xylose, gentiobiose, sophorose, lactose, glycerol, glucose, sorbitol, cellobiose, xylobiose, galactose, laminaribiose, maltose, fructose, mannitol, arabinose, arabinitolo, mannose, sorbose, and a mixture of disaccharides prepared from glucose by incubating glucose with a transglycosylating beta-glucosidase or any combination thereof, and

wherein the mixed whole fermentation broth comprises a ratio of first whole fermentation broth to second whole fermentation broth in a range from 99% (w/w) to 1% (w/w)-70% (w/w) to 30% (w/w).

4. The process according to claim 1 , wherein the first whole fermentation broth comprises a cellobiohydrolase I, a cellobiohydrolase II, an endoglucanase, a beta-glucosidase, a beta-xylosidase, an endoxylanase, a lytic polysaccharide monooxygenase, an acetyl xylan esterase or any combination thereof.

5. The process according to claim 1 , wherein the second whole fermentation broth comprises a cellobiohydrolase I, a cellobiohydrolase II, an endoglucanase, a beta-glucosidase, a beta-xylosidase, an endoxylanase, a lytic polysaccharide monooxygenase, an acetyl xylan esterase or any combination thereof.

6. The process according to claim 3 , wherein the first filamentous fungus is different from the second filamentous fungus.

7. The process according to claim 3 , wherein the first filamentous fungus is identical to the second filamentous fungus.

8. The process according to claim 1 ,

wherein, when culturing the first filamentous fungus, the inducer is present in an amount from 0.01 (w/v) to 10% (w/v), and

wherein when culturing the second filamentous fungus, the inducer is present in an amount less than 0.01% (w/v).

9. The process according to claim 3 ,

wherein, when culturing the first filamentous fungus, the inducer is present in an amount from 0.01 (w/v) to 10% (w/v), and

wherein when culturing the second filamentous fungus, the inducer is present in an amount less than 0.01% (w/v).

Assignments (2)
CONFIRMATORY ASSIGNMENT Recorded Jun 12, 2023
From: DSM IP ASSETS B.V.
To: VERSALIS S.P.A.
Reel/Frame 063957/0001 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 9, 2021
From: PEL, HERMAN JAN; APPELDOORN, MAAIKE; POLDERMANS, ROLF
To: DSM IP ASSETS B.V.
Reel/Frame 057428/0108 →
Priority Claims (1)
EP 19162388 · Mar 12, 2019 · regional
Continuity (1)
Related Publication 20220186264A1 · Jun 16, 2022