UREOLYSIS-INDUCED CALCIUM CARBONATE PRECIPITATION FOR SEALING CHANNELS AND OTHER USES
Methods of conducting ureolysis-induced calcium carbonate precipitation with a heat-treated cell preparation, methods for preparing the heat-treated cell preparation, and related materials. The methods of conducting ureolysis-induced calcium carbonate precipitation include precipitating calcium carbonate at a location by introducing urea, calcium, and a heat-treated cell preparation comprising active urease enzyme to the location. The urease enzyme hydrolyzes the urea to ammonium carbonate, and the calcium reacts with the carbonate to form a calcium carbonate precipitate at the location. The methods of preparing the heat-treated cell preparation include heating a urease-producing cell preparation at a temperature and for a time sufficient to inactivate at least a portion of the cells in the urease-producing cell preparation while maintaining at least some urease activity of urease made by the cells in the urease-producing cell preparation
1 . A method of precipitating calcium carbonate at a location, the method comprising introducing urea, calcium, and a heat-treated cell preparation comprising active urease enzyme to the location, wherein the urease enzyme hydrolyzes the urea to ammonium carbonate, and the calcium reacts with the carbonate to form a calcium carbonate precipitate at the location.
2 . The method of claim 1 , wherein the heat-treated cell preparation includes, in addition to the active urease enzyme, heat-treated cells in the form of intact cells, non-intact remnants thereof, or a mixture thereof.
3 . The method of claim 2 , wherein the heat-treated cell preparation is produced by heating a urease-producing cell preparation.
4 . The method of claim 3 , wherein the heating comprises heating the urease-producing cell preparation at a temperature from about 50° C. to about 70° C. for a time from about 1 minute to about 30 minutes.
5 . The method of claim 3 , wherein the urease-producing cell preparation comprises a urease-producing microbe.
6 . The method of claim 5 , wherein the urease-producing microbe comprises Sporosarcina pasteurii.
7 . The method of claim 3 , wherein the heating is sufficient to inactivate at least a portion of the cells in the preparation while maintaining at least some of the activity of the urease made by the cells.
8 . The method of claim 3 , wherein the heating inactivates at least about 95% of the urease-producing cells in the urease-producing cell preparation.
9 . The method of claim 3 , wherein the heating increases urease activity in the heat-treated cell preparation with respect to urease activity in the urease-producing cell preparation.
10 . The method of claim 3 , wherein the heat-treated cell preparation is introduced to the location after the heating without purification or isolation of components therefrom.
11 . The method of claim 3 , wherein at least one of the urea and the calcium is introduced to the location in a fluid preparation separate from the heat-treated cell preparation.
12 . The method of claim 3 , wherein the location comprises a channel, and the urea, the calcium, and the heat-treated cell preparation is introduced into the channel in amounts and for a time sufficient to at least partially seal the channel.
13 . The method of claim 12 , wherein the channel is a subterranean channel.
14 . The method of claim 12 , wherein the channel is in fluid communication with a wellbore.
15 . The method of claim 12 , wherein the channel comprises a channel in a subterranean formation.
16 . The method of claim 12 , wherein the channel comprises a channel between a cement sheath and a well casing of a subterranean wellbore.
17 . The method of claim 12 , wherein the channel comprises a channel between a cement sheath surrounding a well casing of a subterranean wellbore and a subterranean formation through which the subterranean wellbore is drilled.
18 . The method of claim 12 , wherein the channel comprises a crack in a cement sheath surrounding a well casing of a subterranean wellbore.
19 . A method of preparing a heat-treated cell preparation, the method comprising heating a urease-producing cell preparation at a temperature and for a time sufficient to inactivate at least a portion of the cells in the urease-producing cell preparation while maintaining at least some urease activity of urease made by the cells in the urease-producing cell preparation.
20 . The method of claim 19 , wherein the heating comprises heating the urease-producing cell preparation at a temperature from about 50° C. to about 70° C. for a time from about 1 minute to about 30 minutes.
21 . The method of claim 19 , wherein the urease-producing cell preparation comprises a urease-producing microbe.
22 . The method of claim 21 , wherein the urease-producing microbe comprises Sporosarcina pasteurii.
23 . The method of claim 19 , wherein the heating inactivates at least about 95% of the urease-producing cells in the urease-producing cell preparation.
24 . The method of claim 19 , wherein the heating increases urease activity in the heat-treated cell preparation with respect to urease activity in the urease-producing cell preparation.
25 . The method of claim 19 , further comprising, after the heating, cooling the heat-treated cell preparation to a temperature below about 40° C.