Granted Patent
B2
US 12,571,017 · App. 17/459,053 · Granted Mar 10, 2026
Devices and methods for producing nucleic acids and proteins
Inventors:
Claudia Chiocchini (Regensburg, DE); Axel Trefzer (Tegernheim, DE); Krishna Vattem (Roscoe, IL); Phillip Kuhn (Regensburg, DE)
Assignee:
Life Technologies Corporation
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Abstract
The present disclosure generally relates to devices, compositions and methods for designing and producing nucleic acid molecules and the production of encoded proteins using these nucleic acid molecules. In some aspect, the disclosure relates to automation for the in vitro generation of coding DNA molecules, the in vitro transcription of these DNA molecules to generate protein coding RNA molecules, and the in vitro translation of these protein coding RNA molecules to produce proteins.
Claims (16)
1 . A method of producing a protein, the method comprising:
a) designing a codon optimized nucleic acid molecule, wherein the codon optimized nucleic acid molecule encodes a mammalian protein, and wherein codons of the nucleic acid molecule are optimized for translation in a yeast,
(b) generating oligonucleotides encoding subportions of the codon optimized nucleic acid molecule,
(c) assembling the oligonucleotides generated in (b) to produce the codon optimized nucleic acid molecule,
(d) contacting the codon optimized nucleic acid molecule assembled in (c) with a coupled in vitro translation/transcription reaction mixture under conditions that result in the production of (1) mRNA encoding the protein encoded by the codon optimized nucleic acid molecule and (2) the protein encoded by the codon optimized nucleic acid molecule,
wherein the codons of the nucleic acid molecule are selected to avoid inverse complementary repeats that are capable of forming secondary structures in the mRNA produced in the coupled in vitro translation/transcription reaction mixture, and wherein the reaction mixture of (d) comprises mammalian cell components.
2 . The method of claim 1 , wherein the codon optimized nucleic acid molecule encodes a human protein.
3 . The method of claim 1 , wherein the production of the mammalian protein encoded by the codon optimized nucleic acid molecule optimized for translation in a yeast provides at least 20% higher yield of the mammalian protein than if the codon optimized nucleic acid molecule is optimized for translation in a mammal.
4 . The method of claim 1 , wherein the production of the mammalian protein encoded by the codon optimized nucleic acid molecule optimized for translation in a yeast provides 10% to 40% higher yield of the mammalian protein than if the codon optimized nucleic acid molecule is optimized for translation in a mammal.
5 . The method of claim 1 , wherein the codon optimized nucleic acid molecule has an average GC content of from 35% to 50%.
6 . The method of claim 1 , wherein the codon optimized nucleic acid molecule is linear.
7 . The method of claim 6 , wherein the linear codon optimized nucleic acid molecule comprises a promoter operably linked to the codon optimized nucleic acid molecule.
8 . The method of claim 1 , wherein the reaction mixture of (d) contains exogenously added transfer RNA molecules.
9 . The method of claim 8 , wherein the exogenously added transfer RNA molecules are from yeast.
10 . The method of claim 1 , wherein the yeast is Saccharomyces cerevisiae.
11 . The method of claim 1 , wherein at least half of the codon optimized nucleic acid molecule population is codon optimized for Saccharomyces cerevisiae.
Assignments (3)
MERGER
Recorded Mar 11, 2026
From: PIERCE BIOTECHNOLOGY, INC.
To: LIFE TECHNOLOGIES CORPORATION
Reel/Frame 075052/0213 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 7, 2021
From: CHIOCCHINI, CLAUDIA; TREFZER, AXEL; KUHN, PHILLIP
To: THERMO FISHER SCIENTIFIC GENEART GMBH
Reel/Frame 058328/0910 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Dec 7, 2021
From: VATTEM, KRISHNA
To: PIERCE BIOTECHNOLOGY, INC.
Reel/Frame 058328/0925 →
Continuity (4)
Continuation
15766172
Provisional Application
62334304
· May 10, 2016
Provisional Application
62237764
· Oct 6, 2015
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