Methane monooxygenase enzymes
Improved soluble methane monooxygenases and soluble methane monooxygenase systems are provided.
1. A mutant soluble diiron monooxygenase system, comprising a mutant protein folding chaperone groEL-2 comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 9, wherein the mutant protein folding chaperone groEL-2 comprises a mutation at residue position 409, wherein the residue position is numbered with respect to SEQ ID NO: 9.
2. The mutant soluble diiron monooxygenase system of claim 1 , wherein the mutation is a substitution N409G, wherein the residue position is numbered with respect to SEQ ID NO: 9.
3. One or more isolated nucleic acids encoding one or more polypeptides comprising the mutant soluble diiron monooxygenase system of claim 1 .
4. One or more vectors comprising the one or more isolated nucleic acids of claim 3 .
5. One or more host cells comprising the one or more vectors of claim 4 .
6. The one or more host cells of claim 5 , wherein the one or more host cells comprises a prokaryotic cell.
7. The one or more host cells of claim 6 , wherein the prokaryotic cell is Escherichia coli, Corynebacterium glutamicum , or Bacillus methanolicus.