Systems and methods for fluorescence visualization
A method for fluorescence imaging includes receiving first fluorescence imaging data, receiving a user input selecting a reference region associated with the first fluorescence imaging data, determining a reference fluorescence intensity level based on the reference region, receiving second fluorescence imaging data, determining a relative fluorescence intensity level of a region associated with the second fluorescence imaging data that is relative to the reference fluorescence intensity level, and displaying an indication of the relative fluorescence intensity level.
1. A method for fluorescence imaging comprising:
receiving first fluorescence imaging data corresponding to a first fluorescence image captured during an imaging session, wherein the first fluorescence image is captured following the administration of a fluorescence imaging agent;
receiving a user input selecting a reference region associated with the first fluorescence imaging data;
determining a reference fluorescence intensity level based on the reference region and the first fluorescence imaging data;
receiving second fluorescence imaging data corresponding to a second fluorescence image captured during the imaging session while the fluorescence imaging agent is present in tissue associated with the reference region but after the first fluorescence image was captured;
determining a relative fluorescence intensity level of a region associated with the second fluorescence imaging data that is relative to the reference fluorescence intensity level determined based on the first fluorescence imaging data; and
displaying an indication of the relative fluorescence intensity level.
2. The method of claim 1 , wherein determining the reference fluorescence intensity level comprises determining a mean fluorescence intensity in the reference region and determining the relative fluorescence intensity level comprises determining a mean fluorescence intensity in the region associated with the second fluorescence imaging data.
3. The method of claim 1 , comprising displaying a visualization based on the first fluorescence imaging data, wherein the user input selecting the reference region is a user input selecting a region of the visualization.
4. The method of claim 3 , wherein the visualization comprises video.
5. The method of claim 3 , wherein the visualization comprises fluorescence overlay on visible light imaging.
6. The method of claim 3 , wherein receiving a user input selecting a reference region comprises locating a graphical selector at tissue captured in the first fluorescence imaging data to select a reference region of the tissue.
7. The method of claim 6 , wherein the reference region of the tissue is healthy tissue.
8. The method of claim 1 , wherein the indication of the relative fluorescence intensity level is a numerical indication.
9. The method of claim 1 , comprising receiving third fluorescence imaging data and updating the indication of the relative fluorescence intensity level based on the third fluorescence imaging data.
10. The method of claim 1 , further comprising providing an indication of when the reference fluorescence intensity level was determined.
11. The method of claim 1 , wherein the first and second fluorescence imaging data include video frames.
12. The method of claim 1 , further comprising determining multiple relative fluorescence intensity levels based on multiple regions associated with the second fluorescence imaging data.
13. The method of claim 1 , wherein the reference region and the region associated with the second fluorescence imaging data are associated with the same region of tissue such that the relative fluorescence intensity level indicates a change in fluorescence intensity over time for the region of tissue.
14. The method of claim 1 , wherein the first and second fluorescence imaging data is received from an imager during a medical procedure.
15. The method of claim 1 , wherein the first and second fluorescence imaging data comprise normalized fluorescence intensity data.
16. The method of claim 1 , wherein the user input selecting the reference region associated with the first fluorescence imaging data is received while fluorescence imaging agent is present in tissue associated with the reference region.
17. The method of claim 1 , wherein the reference region and the region associated with the second fluorescence imaging data correspond to the same region of tissue.
18. A system for fluorescence imaging comprising:
one or more processors;
memory; and
one or more programs, wherein the one or more programs are stored in the memory and configured to be executed by the one or more processors, the one or more programs including instructions for:
receiving first fluorescence imaging data corresponding to a first fluorescence image captured during an imaging session, wherein the first fluorescence image is captured following the administration of a fluorescence imaging agent;
receiving a user input selecting a reference region associated with the first fluorescence imaging data;
determining a reference fluorescence intensity level based on the reference region and the first fluorescence imaging data;
receiving second fluorescence imaging data corresponding to a second fluorescence image captured during the imaging session while the fluorescence imaging agent is present in tissue associated with the reference region but after the first fluorescence image was captured;
determining a relative fluorescence intensity level of a region associated with the second fluorescence imaging data that is relative to the reference fluorescence intensity level determined based on the first fluorescence imaging data; and
displaying an indication of the relative fluorescence intensity level.
19. The system of claim 18 , further comprising an imager for generating the first and second fluorescence imaging data.
20. The system of claim 18 , further comprising an illuminator for generating fluorescence excitation light.
21. The system of claim 18 , wherein selecting the target region comprises receiving a user input to select the target region.
22. The system of claim 18 , wherein the first and second fluorescence imaging data comprise normalized fluorescence intensity data.
23. A non-transitory computer readable storage medium storing one or more programs for execution by one or more processors of a fluorescence imaging system, the one or more programs comprising instructions for:
receiving first fluorescence imaging data corresponding to a first fluorescence image captured during an imaging session, wherein the first fluorescence image is captured following the administration of a fluorescence imaging agent;
receiving a user input selecting a reference region associated with the first fluorescence imaging data;
determining a reference fluorescence intensity level based on the reference region and the first fluorescence imaging data;
receiving second fluorescence imaging data corresponding to a second fluorescence image captured during the imaging session while the fluorescence imaging agent is present in tissue associated with the reference region but after the first fluorescence image was captured;
determining a relative fluorescence intensity level of a region associated with the second fluorescence imaging data that is relative to the reference fluorescence intensity level determined based on the first fluorescence imaging data; and
displaying an indication of the relative fluorescence intensity level.