Fluorescence observation device, endoscopic system, processor device, and operation method

1. A fluorescence observation device, comprising:

a signal light source that irradiates a subject with signal light having a wavelength band where an absorption coefficient changes with an oxygen saturation of blood hemoglobin;

an excitation light source that irradiates the subject with excitation light for emitting fluorescence by exciting a fluorescent material contained in the subject;

an image sensor that images the subject with the signal light and outputs a first image signal and that images the subject with the fluorescence and outputs a second image signal; and

a processor unit that

calculates an oxygen saturation of the subject for each pixel based on the first image signal,

sets a reference region of the subject based on the oxygen saturation,

sets a region of interest of the subject,

calculates a normalized fluorescence intensity indicating a normalized emission intensity of the fluorescence by dividing a region-of-interest fluorescence intensity, which is calculated using a pixel value of the region of interest of the subject in the second image signal, by a reference fluorescence intensity calculated using a pixel value of the reference region of the subject in the second image signal, and

generates a fluorescent image of the region of interest which is pseudo colored based on the normalized fluorescence intensity.

2. The fluorescence observation device according to claim 1 ,

wherein the processor unit sets the region of interest based on the oxygen saturation.

3. The fluorescence observation device according to claim 2 ,

wherein the processor unit sets a region where the oxygen saturation falls within a specific range as the reference region.

4. The fluorescence observation device according to claim 2 , further comprising:

a display unit that displays a fluorescent image,

wherein the processor unit obtains a plurality of fluorescent images by imaging the same subject at two or more different times, and

displays the plurality of fluorescent images in time series on the display unit.

5. The fluorescence observation device according to claim 1 ,

wherein the processor unit sets the region of interest based on shape information of the subject.

6. The fluorescence observation device according to claim 5 ,

wherein the processor unit sets a region where the oxygen saturation falls within a specific range as the reference region.

7. The fluorescence observation device according to claim 5 , further comprising:

a display unit that displays a fluorescent image,

wherein the processor unit obtains a plurality of fluorescent images by imaging the same subject at two or more different times, and

displays the plurality of fluorescent images in time series on the display unit.

8. The fluorescence observation device according to claim 1 ,

wherein the processor unit sets a region where the oxygen saturation falls within a specific range as the reference region.

9. The fluorescence observation device according to claim 8 , further comprising:

a display unit that displays a fluorescent image,

wherein the processor unit obtains a plurality of fluorescent images by imaging the same subject at two or more different times, and

displays the plurality of fluorescent images in time series on the display unit.

10. The fluorescence observation device according to claim 1 , further comprising:

a display unit that displays a fluorescent image, wherein the processor unit obtains a plurality of fluorescent images by imaging the same subject at two or more different times, and

displays the plurality of fluorescent images in time series on the display unit.

11. The fluorescence observation device according to claim 1 ,

wherein the processor unit calculates the normalized fluorescence intensity using the region of interest set at a specific time of imaging and the reference region set at each time of imaging.

12. A fluorescence observation device, comprising:

an illumination light source that irradiates a subject, at least a part of which is colored with a colorant, with illumination light by which a color due to the colorant can be identified;

an excitation light source that irradiates the subject with excitation light for emitting fluorescence by exciting a fluorescent material contained in the subject;

an image sensor that images the subject with the illumination light and outputs a first image signal and that images the subject with the fluorescence and outputs a second image signal; and

a processor unit that

sets a reference region according to a color due to the colorant,

sets a region of interest of the subject,

calculates a normalized fluorescence intensity indicating a normalized emission intensity of the fluorescence by dividing a region-of-interest fluorescence intensity, which is calculated using a pixel value of the region of interest of the subject in the second image signal, by a reference fluorescence intensity calculated using a pixel value of the reference region of the subject in the second image signal, and

generates a fluorescent image of the region of interest which is pseudo colored based on the normalized fluorescence intensity.

13. The fluorescence observation device according to claim 12 ,

wherein the processor unit sets the region of interest according to a color due to the colorant.

14. The fluorescence observation device according to claim 12 ,

wherein the colorant is a pH indicator that changes with pH of the subject.

15. The fluorescence observation device according to claim 14 ,

wherein the processor unit sets a region where the pH is equal to or greater than a specific value as the reference region.

16. The fluorescence observation device according to claim 12 ,

wherein the colorant contains at least one of indigo carmine, toluidine blue, methylene blue, Lugol's solution, crystal violet, fluorescein, acridine orange, indocyanine green, or acetic acid.

17. The fluorescence observation device according to claim 12 ,

wherein the processor unit generates the fluorescent image of the region of interest which is pseudo-colored, by using the second image signal obtained from the subject with fluorescence before the colorant is administered to the subject and the first image signal obtained from the subject with the illumination light after the colorant is administered to the subject.

18. An endoscopic system, comprising:

a signal light source that irradiates a subject with signal light having a wavelength band where an absorption coefficient changes with an oxygen saturation of blood hemoglobin;

an excitation light source that irradiates the subject with excitation light for emitting fluorescence by exciting a fluorescent material contained in the subject;

an image sensor that images the subject with the signal light and outputs a first image signal and that images the subject with the fluorescence and outputs a second image signal; and

processor unit that

calculates an oxygen saturation of the subject for each pixel based on the first image signal,

sets a reference region of the subject based on the oxygen saturation,

sets a region of interest of the subject,

calculates a normalized fluorescence intensity indicating a normalized emission intensity of the fluorescence by dividing a region-of-interest fluorescence intensity, which is calculated using a pixel value of the region of interest of the subject in the second image signal, by a reference fluorescence intensity calculated using a pixel value of the reference region of the subject in the second image signal, and

generates a fluorescent image of the region of interest which is pseudo colored based on the normalized fluorescence intensity.

19. A processor device of an endoscopic system including a signal light source that irradiates a subject with signal light having a wavelength band where an absorption coefficient changes with an oxygen saturation of blood hemoglobin, an excitation light source that irradiates the subject with excitation light for emitting fluorescence by exciting a fluorescent material contained in the subject, and an image sensor that images the subject with the signal light and outputs a first image signal and that images the subject with the fluorescence and outputs a second image signal, the processor device comprising:

a processor unit that

calculates an oxygen saturation of the subject for each pixel based on the first image signal,

sets a reference region of the subject based on the oxygen saturation,

sets a region of interest of the subject,

calculates a normalized fluorescence intensity indicating a normalized emission intensity of the fluorescence by dividing a region-of-interest fluorescence intensity, which is calculated using a pixel value of the region of interest of the subject in the second image signal, by a reference fluorescence intensity calculated using a pixel value of the reference region of the subject in the second image signal, and

generates a fluorescent image of the region of interest which is pseudo colored based on the normalized fluorescence intensity.

20. An operation method of a device including a signal light source that irradiates a subject with signal light having a wavelength band where an absorption coefficient changes with an oxygen saturation of blood hemoglobin, an excitation light source that irradiates the subject with excitation light for emitting fluorescence by exciting a fluorescent material contained in the subject, and an image sensor that images the subject with the signal light and outputs a first image signal and that images the subject with the fluorescence and outputs a second image signal, the operation method comprising:

an oxygen saturation calculation step of calculating an oxygen saturation of the subject for each pixel based on the first image signal;

a reference region setting step of setting a reference region of the subject based on the oxygen saturation;

a region-of-interest setting step of setting a region of interest of the subject;

a normalized fluorescence intensity calculation step of calculating a normalized fluorescence intensity indicating a normalized emission intensity of the fluorescence by dividing a region-of-interest fluorescence intensity, which is calculated using a pixel value of the region of interest of the subject in the second image signal, by a reference fluorescence intensity calculated using a pixel value of the reference region of the subject in the second image signal; and

a fluorescent image generation step of generating a fluorescent image of the region of interest which is pseudo colored based on the normalized fluorescence intensity.