Systems and methods for rapid nucleic acid extraction, purification and analysis from semen
Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.
1. A process for extracting nucleic acid from a semen sample, comprising:
a. collecting said semen sample onto a swab;
b. adding approximately 50 μl of a sperm disruptive agent to said swab, wherein said sperm disruptive agent is DTT in approximate concentration of 150 mM or TCEP in an approximate concentration of 50 mM;
c. incubating said swab and said sperm disruptive agent mixture for one minute or less;
d. transferring said swab directly into a rapid DNA processing system for further analysis without purification;
e. obtaining at least one STR profile from said sample using said rapid DNA processing system.
2. The process of claim 1 , wherein the semen sample is neat semen or a semen stain.
3. The process of claim 1 , wherein at least seven STR loci are interrogated.