Granted Patent
B2
US 12,416,005 · App. 17/515,139 · Granted Sep 16, 2025
Pig with a genetically modified CD163 gene resistant to PRRSv
Inventors:
Andrew Mark Cigan (Madison, WI); Jonathan Edward Lightner (Jefferson, WI); Matthew Scott Culbertson (Wilmington, NC); William Thomas Christianson (Hendersonville, TN); Benjamin Beaton (DeForest, WI); Brian Burger (Madison, WI); Dylan Barnes (Madison, WI); Matthew Campbell (Madison, WI)
Assignee:
Genus plc
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Abstract
The present disclosure relates to methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species Sus scrofa . The present teachings relate to swine wherein at least one allele of a CD163 gene has been inactivated, and to specific methods and nucleic acid sequences used in gene editing to inactivate the CD163 gene. Swine wherein both alleles of the CD163 gene are inactivated are resistant to porcine reproductive and respiratory syndrome virus (PRRSv). Elite lines comprising homozygous CD163-edited genes retain their superior properties.
Claims (6)
1. A method of screening comprising:
i) breeding a genetically edited pig whose genome comprises a CD163 gene that has a homozygous deletion of exon 7 and comprises the nucleic acid sequence of SEQ ID NO: 453, wherein the pig exhibits resistance to porcine reproductive and respiratory syndrome virus (PRRSV) such that offspring are obtained;
ii) screening the genomes of the offspring for the presence of a CD163 gene that has a homozygous deletion of exon 7 and comprises the nucleic acid sequence of SEQ ID NO: 453; and
iii) screening the offspring for resistance to PRRSV.
2. The method of claim 1 , wherein the screening is performed using polymerase chain reaction (PCR) and primers comprising the nucleic acid sequences of SEQ ID NO: 556 and 557.
3. The method of claim 1 , wherein the screening is performed using polymerase chain reaction (PCR) and primers comprising the nucleic acid sequences of SEQ ID NO: 562 and 563.
Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 12, 2022
From: BEATON, BENJAMIN; BURGER, BRIAN
To: GENUS PLC
Reel/Frame 058626/0542 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 12, 2022
From: BARNES, DYLAN; CAMPBELL, MATTHEW
To: GENUS PLC
Reel/Frame 058626/0549 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jan 11, 2022
From: CIGAN, ANDREW MARK; LIGHTNER, JONATHAN EDWARD; CULBERTSON, MATTHEW SCOTT; CHRISTIANSON, WILLIAM THOMAS
To: GENUS PLC
Reel/Frame 058625/0158 →
Continuity (4)
Provisional Application
63021370
· May 7, 2020
Provisional Application
63020128
· May 5, 2020
References Cited (245)
US 4873191A
· Wagner et al.
· 1989
[cited by applicant]
US 9820475B2
· Prather et al.
· 2017
[cited by applicant]
WO WO2017023337
· 2017
[cited by applicant]
WO WO2018073237
· 2018
[cited by examiner]
WO WO2019203807
· 2019
[cited by applicant]
Yang, Dongshan, et al., Generation of PPARy mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning, Cell Research, 2011, 21:979-982.
[cited by applicant]
Yoon, In J., et al., Persistent and contact infection in nursery pigs experimentally infected with reproductive and respiratory syndrome (PPRS) virus, Swine Health and Production, 1993, 1(4):5-8.
[cited by applicant]
Yoshioka, Kojn, et al., Birth of Piglets Derived from Porcine Zygotes Cultured in a Chemically Defined Medium, Biol of Reprod, 2002, 66:112-119.
[cited by applicant]
Zhang, Qingzhan and Yoo, Dongwan, PRRS virus receptors and their role for pathogenesis, Veternary Microbiology, 2015, 177:229-241.
[cited by applicant]
Zhao, Jianguo, et al., Histone Deacetylase Inhibitors Improve In Vitro and In Vivo Development Competence of Somatic Cell Nuclear Transfer Porcine Embryos, Cellular Reprogramming, 2010, 12(1):75-83.
[cited by applicant]
Zhao, Jianguo, et al., Significant Improvement in Cloning Efficiency of an Inbred Miniature Pig by Histone Deacetylase Inhibitor Treatment after Somatic Cell Nuclear Transfer, Biology of Reproduction, 2009, 81:525-530.
[cited by applicant]
Abeydeera, L.R. and Day, B.N., Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa,
[cited by applicant]
Agung et al., In vitro fertilization and development of porcine oocytes matured in follicular fluid,
[cited by applicant]
Alkan, F., et al., CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters, Genome Biol., 2018, 19:177.
[cited by applicant]
Altschul, S.P., et al., Basic local alignment search tool,
[cited by applicant]
Appeltant et al., Porcine oocyte maturation in vitro: role of cAMP and oocyte-secreted factors—A practical approach,
[cited by applicant]
Bae, S., et al., Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases, Bioinformatics, 2014, 30:1473-1475.
[cited by applicant]
Cameron, P., et al., Mapping porcine landscape of CRISPR-Cas9 cleavage, Nat Methods 2017, 14:600-606.
[cited by applicant]
Chenna, R., et al., Multiple sequence alignment with the Clustal series of programs,
[cited by applicant]
Fowler et al., The production of pig preimplantation embryos in vitro: Current progress and future prospects,
[cited by applicant]
Fonfara et al., Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems,
[cited by applicant]
Gnirke, A., et al., Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing, Nat. Biotechnol., 2009, 27:182-189.
[cited by applicant]
Guo, Chunhe, et al., Highly Efficient Generation of Pigs Harboring a Partial Deletion of the CD163 SRCR5 Domain, Which are Fully Resistant to Porcine Reproductive and Respiratory Syndrome Virus 2 Infection, Frontiers in…
[cited by applicant]
Haft, Daniel H., et al., A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes,
[cited by applicant]
Jinek, M., et al., A programmable dual RNA-guided DNA endonuclease in adaptive bacterial immunity,
[cited by applicant]
Joung et al., TALENs: a widely applicable technology for targeted genome editing,
[cited by applicant]
Kang, Soo Ji, et al., Comparision of Seven Commercial TaqMan Master Mixes and Two Real-Time PCR Platforms Regarding the Rapid Detection of Porcine DNA, Food Sci Anim Resour, 2021, 41(1):85-94.
[cited by applicant]
Karvelis et al., Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements,
[cited by applicant]
Larkin Ma et al., Clustal W and Clustal X version 2.0,
[cited by applicant]
Lavitrano et al., Efficient production by sperm-mechiated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation,
[cited by applicant]
Lavitrano et al., Sperm-mediated gene transfer,
[cited by applicant]
Lo, Transformation by iontophoretic microinjection of DNA: multiple integrations without tandem insertions,
[cited by applicant]
Onofre, Gabriela et al., Scavenger receptor CD163 and its biological functions,
[cited by applicant]
Ran, F. A., et al., In vivo genome editing using
[cited by applicant]
Sapranauskas et al., The
[cited by applicant]
Shah et al., Protospacer recognition motifs: mixed identities and functional diversity,
[cited by applicant]
Suzuki, C., et al., Effects of amino acid supplements and replacement of polyvinyl alcohol with bovine serum albumin in porcine zygote medium, Reprod. Fertil. Dev., 2006 18:789-795.
[cited by applicant]
Svitashev et al., Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes, Nature Communications, 2016, 7:113274.
[cited by applicant]
Thompson et al., Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells,
[cited by applicant]
Thompson, J.D. and Clustal, W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice et al.,
[cited by applicant]
Tsai, S.Q., et al., GUIDE-seg enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases, Nat. Biotechnol. 2015, 33;187-197.
[cited by applicant]
Tsai, S.Q., et al., CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR-Cas9 nuclease off-targets, Nat. Methods, 2017, 14;607-614.
[cited by applicant]
Van Der Putten et al., Efficient insertion of genes into the mouse germ line via retroviral vectors,
[cited by applicant]
Wakayama et al., Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei,
[cited by applicant]
Wilmut et al., Viable offspring derived from fetal and adult mammalian cells, Nature,
[cited by applicant]
Yoshioka, K., et al., Birth of piglets derived from porcine zygotes cultured in a chemically defined medium, Biol. Reprod., 2002, 60:112-119.
[cited by applicant]
Yoshioka, K., Defined System for In Vitro Production of Porcine Embryos Using a Single Basic Mediom, J. Reprod. Dev. 2008, 54:208-213.
[cited by applicant]
Yoshioka, K., J., Development and application of a chemically defined medium for the in vitro production of porcine embryos,
[cited by applicant]
Zetsche et al., Cpfl is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system,
[cited by applicant]
Zhang et al., Off-target Effects in CRISPR/Cas9-mediated Genome Engineering,
[cited by applicant]
Zhao, C., et al., CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif, Int. J. Biol. Sci., 2017, 13:1470-1478.
[cited by applicant]
Merck Sharp and Dohme Corp., Normal Rectal Temperature Ranges, 2009, pp. I.
[cited by applicant]
Miao, Yi-hang, et al., Centrosome Abnormalilties During Porcine Oocyte Aging, Environmental and Molecular Mutagenesis, 2009, 50:666-671.
[cited by applicant]
Misinzo, Gerald, M., et al., Involvement of proteases in porcine reproductive and respiratory syndrome virus uncoating upon internalization in primary macrophages, Vet. Res., 2008, 39:55-69.
[cited by applicant]
Molitor, T.W., et al., Immunity to PRRSV: Double-edged sword, Veterinary Microbiology, 1997, 55:265-276.
[cited by applicant]
Morgan, S.B., et al., Pathology and Virus Distribution in the Lung and Lymphoid Tissues of Pigs Experimentally Inoculated with Three Distinct Type 1 PRRS Virus Isolates of Varying Pathogenicity, Transboundary and Emergi…
[cited by applicant]
Murtaugh, M.P., et al., Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus, Arch Virol, 1995, 140:1451-1460.
[cited by applicant]
Nath, Deepa, et al., The Amino-terminal Immunoglobulin-like Domain of Sialoadhesin Contains the Sialic Acid Binding Site, J Biol Chem, 1995, 270(44):26184-26191.
[cited by applicant]
Nauwynck, H.J., et al, Entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages via receptor-medicated endocytosis, J General Virology, 1999, 80:297-305.
[cited by applicant]
Nelson, Chris J., et al., Porcine Reproductive and Respiratory Syndrome Virus Comparison: Divergent Evolution on Two Continents, J Virology, 999, 73(1):270-280.
[cited by applicant]
Neumann, Eric J., et al., Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States, JAVMA, 2005, 227(3):385-392.
[cited by applicant]
Nielsen, H.S., et al., Generation of an Infectious Clone of VR-2332, a Highly Virulent North American-Type Isolate of Porcine Reproductive and Respiratory Syndrome Virus, J Virology, 2003, 77(6):3702-3711.
[cited by applicant]
Nielsen, Marianne Jensby, et al., The macrophage scavenger receptor CD163: endocytic properties of cytoplasmic tail variants, J Leukocyte Biology, 2006, 79:837-845.
[cited by applicant]
Niu, Yaya, et al., Generation of Gene-Modified Cynomolgus Monkeys via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos, Cell, 2014, 156:836-843.
[cited by applicant]
Nussbaum, Diana J. and Prather, Randall S., Differential Effects of Protein Synthesis Inhibitors on Porcine Oocyte Activation, Mol Reprod and Develop, 1995, 41:70-75.
[cited by applicant]
Oetke, Cornelia, et al., Sialoadhesin-Deficient Mice Exhibit Subtle Changes in B- and T-Cell Populations and Reduced Immunoglobulin M Levels, Mol and Cell Biol, 2006, 26(4):1549-1557.
[cited by applicant]
Park, Kwang-Wook, et al., Developmental Potential of Porcine Nuclear Transfer Embryos Derived from Transgenic Fetal Fibroblasts Infected with the Gene for the Green Fluorescent Protein: Comparison of Different Pasion/Ac…
[cited by applicant]
Park, Kwang-Wook, et al., Production fo Nuclear Transfer-Derived Swine That Express the Enhanced Green Fluorescent Protein, Animal Biotech, 2001, 12(2). 173-181.
[cited by applicant]
Patience, John F. and Thacker, Phil A., Nutrition of the Breeding Herd, Prairie Swine Centre, 1989, pp. 149-171.
[cited by applicant]
Patton, John B., et al., Modulation of CD163 receptor expression and replication of porcine reproductive and respiratory syndrome virus in porcine macrophages, Virus Research, 2009, 140:161-171.
[cited by applicant]
Plagemann, Peter O.W., Lactage Dehydrogenase-Elevating Virus and Related Viruses, Fields Virology, 3
[cited by applicant]
Popescu, Luca, et al., Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia Jan. 2007, Virology, 2017, 501:102-106.
[cited by applicant]
Prather, Randall S., et al., Genetic engineering alveolar macrophages for host resistance to PRRSV, Veterinary Microbiology, 2017, 209:124-129.
[cited by applicant]
Prather, Randall S., et al., Knockout of matemal CD163 protects fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV), Nature Scientific Reports, 2017, 7(13371):1-5.
[cited by applicant]
Prather, R.S., et al., Genetic Engineering of Pigs for PRRSV Resistance, Animal Sciences, Univ Missouri-Columbia, College of Ag Food and Natural Resources, Animal Reproductive Biology Group, Slides, pp. 1-43.
[cited by applicant]
Prather, Randall S., et al., An Intact Staloadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus, J Virology, 2013, 87(17):9538-9546.
[cited by applicant]
Prather, R.S., Genetic Engineering of Pigs for PRRSV resistance, Animal Sciences, Univ Missouri-Columbia, College of Ag Food and Natural Resources, Animal Reproductive Biology Group, Slides, pp. 1-55.
[cited by applicant]
Prather, R.S., et al., Genetic engineering the pig to better understand PRRSv infection, 2014 North American PRRS Symposium, 2014, p. 33.
[cited by applicant]
Prather, R.S., Genetic Engineering for Profitable Port Production: Is Resistance to PRRSv Possible?, Animal Sciences, Univ Missouri-Columbia, College of Ag Food and Natural Resources, Animal Reproductive Biology Group, …
[cited by applicant]
Prather, Randall S., et al. CRISPR/Cas9-Mediated Genetic Engineering: Is CD163 an Entry Mediator for PRRSv Infection?, International Plant and Animal Genome Conference XXIII, Abstract, Jan. 9-15, 2015, 1 page.
[cited by applicant]
Provost, Chantale, et al., Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line, Virology…
[cited by applicant]
Reed, L.J. and Muench, H., A Simple Method of Estimating Fifty Per Cent Endpoints, American Journal of Hygiene, 1938, 27(3):493-497.
[cited by applicant]
Rezaee, Ramin and Abdollahi, Mohammad, The Importance of Transistability in Drug Discovery, Expert Opinion on Drug Discovery, 2017, 12(3):237-239.
[cited by applicant]
Ritter, M., et al., The Scavenger Receptor CD163: Regulation, Promoter Structure and Genomic Organization, Pathobiology, 1999, 67:257-261.
[cited by applicant]
Ritter, Mirko, et al., Genomic Organization and Chromosomal Localization of the Human CD163 (M130) Gene: A Member of the Scavenger Receptor Cysteine-Rich Superfamily, Biochem and Biophysical Research Communications, 199…
[cited by applicant]
Robl, J.M., et al., Nuclear Transplantation in Bovine Embryos, J Anim Sci., 1987, 64:642-647.
[cited by applicant]
Ropp, Susan L., et al., Characterization of Emerging European-Like Porcine Reproductive and Respiratory Syndrome Virus Isolates in the United States, J Virology, 2004, 78(7):3684-3703.
[cited by applicant]
Ross, Jason W., et al., Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts, Transgenic Res, 2010, 19(4):611-620.
[cited by applicant]
Rowland, Raymond R.R., et al., Control of porcine reproductive and respiratory syndrome (PRRS) through genetic improvements in disease resistance and tolerance, Frontiers in Genetics, 2012, 3(260):1-6.
[cited by applicant]
Rowland, Raymond R.R., et al., The Evolution of Porcine Reproductive and Respiratory Syndrome Virus: Quasispecies and Emergence of a Virus Subpopulation during Infection of Pigs with VR-2332, Virology, 1999, 259:262-266.
[cited by applicant]
Saeidnia, Soodabeh, et al., From in vitro Experiments to in vivo and Clinical Studies; Pros and Cons, Current Drug Discovery Technologies, 2015, 12(4):218-224.
[cited by applicant]
Sanchez, Carmen, et al., The Porcine 2A10 Antigen Is Homologous to Human CD163 and Related to Macrophage Differentiation, J Immunology, 19 162:5230-5237.
[cited by applicant]
Sanchez-Torres, C., et al., Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection, Arch Virol, 2003, 148:2307-2323.
[cited by applicant]
Sangamo BioSciences, Efficient Generation of Transgenic Pigs Using Zinc Finger Nuclease (ZFN) Technology, Demonstration of ZEN Technology for Efficient Creation of Animals as a Source of Organs for Transplantation into …
[cited by applicant]
Schaer, Christian A., et al., Constitutive Endocytosis of CD163 Mediates Hemoglobin-Heme Uptake and Determines the Noninflammatory and Protective Transcriptional Response of Macrophages to Hemoglobin, Circ Res, 2006, 99…
[cited by applicant]
Schaer, Dominik J., et al., CD163 is the macrophage scavenger receptor for native and chemically modified hemoglobins in the absence of haptoglobin, Blood, 2006, 107(1):373-380.
[cited by applicant]
Schaer, DJ, et al., Hemophagocytic macrophages constitute a major compartment of hemne oxygenase expression in sepsis, Eur J Haematol, 2006, 77:432-436.
[cited by applicant]
Schurgers, Evelien, et al., Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis, Arthritis Research & Therapy, 2010, 12(R31):3-11.
[cited by applicant]
Aigner, Bernhard, et al., Transgenic pigs as models for translational biomedical research, J Mol Med, 2010, 88:653-664.
[cited by applicant]
Albina, E., et al., Immune response and persistence of the porcine reproductive and respiratory syndrome virus in infected pigs and far units, The Veterinary Record, May 28, 1994, 134.567-573.
[cited by applicant]
Allende, R., et al., North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions, J General Virology, 1999, 80:307-315.
[cited by applicant]
Allende, R., et al., Porcine Reproductive and Respiratory Syndrome Virus: Deseption of Persistence in Individual Pigs upon Experimental Infection, J Virology, Nov. 2000, 74(22):10834-10837.
[cited by applicant]
American Society for Cell Biology, “Small details between ‘in vivo’ and ‘in vitro’ studies make for big differences.” ScienceDaily. ScienceDaily, Dec. 13, 2010.
[cited by applicant]
Andreyev, V.G., et al., Genetic variation and phylogenetic relationships of 22 poreine reproductive and respiratory syndrome virus (PRRSV) field strains based on sequence analysis of open reading frame 5, Arch Virol 199…
[cited by applicant]
ATCC CRL-3216, 293T, Organism:
[cited by applicant]
Bauer, B.K., et al., l Arginine Supplementation in vitro Increases Porcine Embryo Development and Affects mRNA Transcript Expression, Reproduction, Fertility and Development, Dec. 7, 2010, 23(1):107.
[cited by applicant]
Beaton, Benjamin P. and Wells, Kevin D., Compound Transgenics: Recombinase-Mediated Gene Stacking, Transgenic Animal Technology, pp. 565-678.
[cited by applicant]
Benfield, David A., et al., Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332), J Vet Diagn Invest, 1992 4:127-133.
[cited by applicant]
Benfield, David A., et al., Pathogenesis and persistence of PRRS, Allen D. Leman Swine Conference, 1998, pp. 169-171.
[cited by applicant]
Berg, H., 200-Biological Implications of Electric Field Effects Part V. Fusion of Blastomeres and Blastocysts of Mouns Embryos, Bioelectrochemistry and Bioenergetics, 1982, 9:223-228.
[cited by applicant]
Boddicker, Nicholas J., et al., Genome-wide association and genomic prediction for host response to porcine reproductive and respiratory syndrome virus infection, Genetics Selection Evolution, 2014, 46(18):1-14.
[cited by applicant]
Bookstein, Robert, et al., Promoter deletion and loss of retinoblastoma gene expression in human prostate carcinoma, Proc. Natl. Acad. Sci. USA, 1990, 87:7762-7766.
[cited by applicant]
Borg, Natalie A., et al., CD1d-lipid-antigen recognition by the semi-invariant NKT T-cell receptor, Nature, Jul. 5, 2007, 448:44-49.
[cited by applicant]
Brinster, Ralph L., et al., Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs, Proc. Natl. Acad. Sci., Jul. 1985, 82:4438-4442.
[cited by applicant]
Burkard, Christine, et al., Pigs Lacking the Scavenger Receptor Cysteme-Rich Domain 5 of CD163 Are Resistant to Procine Reproductive and Respiratory Syndrome Virus 1 Infection, J. Virology, 2018, 92(16):1-17.
[cited by applicant]
Burkard, Christine, et al., Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological fun…
[cited by applicant]
Burlac, C., et al., Identification of human preformed antibody targets in GTKO pigs, Xenotransplantation 2012, 19:92-101
[cited by applicant]
Calvert, Jay G., et al., CD163 Expression Confers Susceptibility to Porcine Reproductive and Respiratory Syndrome Viruses, J of Virology, Jul. 2007, 81(14):7371-7379.
[cited by applicant]
Carter, D. Bart, et al., Phenotyping of Transgenic Cloned Piglets, Cloning and Stem Cells, 2002, 4(2):131-145.
[cited by applicant]
Chin, Kun-Kuet and Shwu-Pen Chang, The −104G nucleotide of the human CYP21 gene is important for CYP21 transcription activity and protein interaction, Nucleic Acids Research, 1998, 26(8):1959-1964.
[cited by applicant]
Christopher-Hennings, Jane, et al., Persistence of porcine reproductive and respiratory syndrome virus in serum and semen of adult boars, J Vet Diagn Invest, 1995, 7:456-464.
[cited by applicant]
Christopher-Hennings, J, et al., Effects of a modified-live virus vaccine against porcine reproductive and respiratory syndrome in boars, AJVR, 1997, 58(1):40-45.
[cited by applicant]
Ciotti, M., et al., Coding defect and a TATA box mutation at the bilirubin UDP-glucuronosyltransferase gene canse Crigler-Najjar type 1 disease, Biochimica et Biophysica Acta, 1998, 1407:40-S0.
[cited by applicant]
Cong, Le, et al., Multiplex Genome Engineering Using CRISPR/Cas Systems, Science, Feb. 15, 2013, 339(6121):819-823.
[cited by applicant]
Cooper, David K C. Modifying the sugar icing on the transplantation cake, Glycobiology, 2016, 26(6):571-581.
[cited by applicant]
Crocker, Paul R. and Gordon, Siamon, Properties and Distribution of a Lectin-Like Hemagglutinin Differentially Expressed by Murine Stromal Tissue Macrophages, J Exp Med, The Rockefeller Univ. Press, Dec. 1986, 164:1862-…
[cited by applicant]
Crocker, Pant R., et al., Molecular analysis of sialoside binding to sialoadhesin by NMR and site-directed mutagenesis, Biochem J, 1999, 341:355-361.
[cited by applicant]
Dai, Yifan, et al., Targeted disruption of the α1, 3-galactosyltransferase gene in cloned pigs, Nature, Mar. 2002, 20:251-255.
[cited by applicant]
Das, Phani B., et al., The Minor Envelope Glycoproteins GP2a and GP4 of Porcine Reproductive and Respiratory Syndrome Virus Interact with the Receptor CD163, J Virology, Feb. 2010, 84(4):1731-1740
[cited by applicant]
Dee, S. A. and Molitor, T. W., Elimination of porcine reproductive and respiratory syndrome virus using a test and removal process, Veterinary Record, 1998, 143:474-476.
[cited by applicant]
Delputte, P.L., et al., Effect of virus-specific antibodies on attachment, internalization and infection of porcine reproductive and respiratory syndrome virus in primary macrophages, Vet Immunology and Immunopathology,…
[cited by applicant]
Delputte, P.L., et al., Involvement of the Matrix Protein in Attachment of Porcine Reproductive and Respiratory Syndrome Virus to a Heparinlike Receptor on Porcine Alveolar Macrophages, J Virology, May 2002, 76(9) 4312-…
[cited by applicant]
Delputte, P.L., et al., Poreine Arterivirus Infection of Alveolar Macrophages Is Mediated by Sialic Acid on the Virus, J Virology, Aug. 2004, 78(15):8094-8101.
[cited by applicant]
Delputte, P.L., et al., Porcine Arterivirus Attachment to the Macrophage-Specific Receptor Sialoadhesin Is Dependent on the Sialic Acid-Binding Activity of the N-Terminal Immunoglobulin Domain of Sialoadhesin, J Virolog…
[cited by applicant]
Delputte, P.L., et al., Porcine Sialoadhesin (CD169/Siglec-1) Is an Endocytic Receptor that Allows Targeted Delivery of Toxins and Antigens to Macrophages, PLoS ONE, Feb. 2011, 6(2):1-12.
[cited by applicant]
Etzerodt, Anders, et al., Plasma Clearance of Hemoglobin and Haptoglobin in Mice and Effect of CD163 Gene Targeting Disruption, Antioxidants & Redox Signaling, 2013, 18(17):2254-2263.
[cited by applicant]
Etzerodt, Andres and Moestrup, Soren K., CD163 and Inflammation: Biological, Diagnostic, and Therapeutic Aspects, Antioxidants & Redox Signaling, 2013, 18(17):2352-2363.
[cited by applicant]
Fabriek, Babs O., et al., The macrophage scavenger receptor CD163, Immunobiology, 2005, 210:153-160
[cited by applicant]
Fabriek, Babs O., et al., The macrophage scavenger receptor CD163 functions as an innate immune sensor for bacteria, Blood Journal, Jan. 22, 2009, 113(4):887-892.
[cited by applicant]
Fisher, D. and Goodall, A.H., Membrane fusion by viruses and chemical agents, Techniques in Cellular Physiology, 1981, P115:1-36.
[cited by applicant]
Fridman, AL and Tainsky, MA, Critical pathways in cellular senescence and immortalization revealed by gene expression profiling, Oncogene, 2008, 27:5975-5987.
[cited by applicant]
Gaj, Thomas, et al., ZEN, TALEN and CRISPR/Cas-based methods for genome engineering, Trends Biotechnol, 2013, 31(7):397-405.
[cited by applicant]
Galili, Uri, Xenotransplantation and ABO incompatible transplantation: The similarities they share, Transfusion and Apheresis Science, 2006, 35:45-58.
[cited by applicant]
Gaudreault, N., et al., Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus, Arch Virol, 2009, 154:133-136.
[cited by applicant]
Gerrits, Roger J., et al., Perspectives for artificial insemination and genomics to improve global swine populations, Theriogenology, 2005. 63:283-299.
[cited by applicant]
Goryenin, Igor, et al., Applications of Whole Cell and Large Pathway Mathematical Models in the Pharmaceutical Industry, Chapter 12, Metabolic Engineering in the Post Genomic Era, Horizon Bioscience, 2004, p. 344.
[cited by applicant]
Graham, Christopher F., The Fusion of Cells with One- and Two-Cell Mouse Embryos, Wistar Inst Symp Monogr., 1969, 9:19-35.
[cited by applicant]
Graversen, Jonas H., et al., Targeting the Hemoglobin Scavenger receptor CD163 in Macrophages Highly Increases the Anti-Inflammatory Potency of Dexamethasone, Molecular Therapy, Ang. 2012, 20(8):1550-1558.
[cited by applicant]
Groenen, Martien A. M., et al., Analyses of pig genomes provide insight into porcine demography and evolution, Nature, Nov. 15, 2012, 491(7424):393-398.
[cited by applicant]
Hai, Tang et al., One-step generation of knockout pigs by zygote injection of CRISPR/Cas System, Cell Research, 2014, 34:372-375.
[cited by applicant]
Halbur, P.O., et al., Comparison of the Pathogenicity of Two US Porcine Reproductive and Respiratory Syndrome Virus Isolates with that of the Lelystad Virus, Vet Pathol, 1995, 32:648-660.
[cited by applicant]
Hammer, Robert E., et al., Production of transgenic rabbits, sheep and pigs by microinjection, Nature, Jun. 1985, 315:680-683.
[cited by applicant]
Hao, Y.H., et al., Production of endothelial nitric oxide synthase (eNOS) over-expressing piglets, Transgenic Res, 2006, 15:739-750.
[cited by applicant]
Hauschild, Janet, et al., Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases, PNAS, 2011, 108(36):1-6.
[cited by applicant]
Holtkamp, Derald J., et al., Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States port producers, J Swine Health and Production, 2013, 21(2):72-84.
[cited by applicant]
Huang, Y.W., et al., Porcine DC-SIGN: Moleenlat cloning, gene structure, tissue distribution and binding characteristics, Dev and Comparative Immunology, 2009, 33:464-480.
[cited by applicant]
Hwang, Woong Y, et al., Efficient genome editing in zebrafish using a CRISPR-Cas system, Nature Biotechnology, 2013, 31(3):227-229.
[cited by applicant]
Hyder, Salvoan M., et al., Identification of an Estrogen Response Element in the 3′-Flanking Region of the Murine e-fos Protooncogene, J Biological Chemistry, 1992, 267(25):18047-18054.
[cited by applicant]
Im, Gi-Sun, et al., In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres, Theriogenology, 2004, 61:1125-1135.
[cited by applicant]
Jeney, Viktona, et al., Pro-oxidant and cytotoxic effects of circulating heme, 2002, 100(3):879-887.
[cited by applicant]
Kefpaber, Kerry K., Reproductive Failure of Unknown Etiology, 1989, 1(2):1-10.
[cited by applicant]
Kim, Hyongbum and Kim, Jin-Soo, A guide to genome engineering with programmable nucleases, Nature Reviews Genetic, 2014, 15:321-334.
[cited by applicant]
Kim, H.S., et al., Enhanced replication of poreme reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line, Arch Vitol, 1993, 133:477-483.
[cited by applicant]
Kim, Jeong-Ki, et al., Defining the Cellilat Target(s) of Porcine Reproductive and Respiratory Syndrome Virus Blocking Monoclonal Antibody 7G10, J Virology, 2006, 80(2):689-696.
[cited by applicant]
Kleinjan, Dirk-Jan and Coutinho, Pedro, Cis-ruption mechanisms: disruption of cis-regulatory control as a cause of human genetic disease, Briefings Functional Genomics and Proteomics, 2009, 8(4):317-332.
[cited by applicant]
Kolston, Paul J., Comparing in vitro, in situ, and in vivo experimental data in a three-dimensional model of mammalian cochlear mechanics, Proc Natl Acad Sci, 1999, 96:3676-3681.
[cited by applicant]
Kristiansen, Mette, et al., Identification of the haemoglobin scavenger receptor, Nature, 2001, 409: 198-201.
[cited by applicant]
Kwon, Deug-Nam, et al., Production of biallelic CMP-Neu5Ac hydroxylase knock-out pigs, Scientific Reports, 2013, 3(1981)1-10.
[cited by applicant]
Ladinig, Andrea, et al., Pathogenicity of three type 2 porcine reproductive and respiratory syndrome virus strains in experimentally inoculated pregnant gilts, Virus Research, 2015, 203:34-35.
[cited by applicant]
Lager, Kelly M., et al., Evaluation of protective immunity in gilts inoculated with the NADC-8 isolate of porcine reproductive and respiratory syndrome virus (PRRSV) and challenge-exposed with an antigenically distinct …
[cited by applicant]
Lai, Liangxue, et al., Generation of cloned transgenic pigs rich in omega-3 fatty acids, Nature Biotechnology, 2006, 24(4):435-436.
[cited by applicant]
Lai, Liangxue, et al., Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning, Science, 2002, 295:1089-1092.
[cited by applicant]
Lai, Liangxne, and Prather, Randall S., Creating genetically modified pigs by using nuclear transfer, Reprod Biol and Endocrinology, 2003, 1(82):1-6.
[cited by applicant]
Lai, Liangxue and Prather, Randall S., Production of Cloned Pigs by Using Somatic Dells as Donors, Cloning and Stem Cells, 2003, 5(4):233-241.
[cited by applicant]
Lai, Liangxue and Prather, Randall S., A method for Producing Cloned Pigs by Using Somatic Cells as Donors, Methods in Molecular Biology, Germ Cell Protocols, 2004, vol. 2, Ch. 9, pp. 149-163.
[cited by applicant]
Lawson, Stevewn R., et al., Porcine reproductive and respiratory syndrome virus infection of gnotobiotic pigs: sites of virus replication and co-localization with MAC-387 staining at 21 days post-infection, Virus Resear…
[cited by applicant]
Lee, Kiho, et al., Engraftment of human iPS cells and allogeneic porcine cells into pigs with inactivated RAG2 and accompanying severe combined immunodeficiency, PNAS, 2014, 111(20):7260-7265.
[cited by applicant]
Lee, Kiho, et al., Piglets Produced From Cloned Blastocysts Cultured In Vitro With GM-CSF, Mol Reprod, Dev, 2013, 80(2):145-154.
[cited by applicant]
Li, Dali, et al., Heritable gene targeting in the mouse and rat using a CRISPR-Cas system, Nature Biotechnology, 2013, 31(8):681-683.
[cited by applicant]
Li, Rongfeng, et al., Cloned Transgenic Swine Via In Vitro Production and Cryopreservation, Biology of Reprod, 2006, 75:226-230.
[cited by applicant]
Li, Ying and Jaiswal, Anil K., Regulation of Human NAD(P)H:Quinone Oxidereductase Gene, J Biol Chem, 1992, 267(21):15097-15104.
[cited by applicant]
Lillico, Simon G., et al., Live pigs produced from genome edited zygotes, Scientific Reports, 2013, 3:2847-2850
[cited by applicant]
Loudianos, O., et al., Molecular characterization of Wilson disease in the Sardinian population—evidence of a founder effect, Hum Matat, 1999, 14(4):294-303.
[cited by applicant]
Lunney, Joan K. and Chen, Hongbo, Genetic control of host resistance to porcine reproductive and respiratory syndrome virus (PRRSV) infection, Virus Research, 2010, 154:161-169
[cited by applicant]
MacHaty, Zoltan, et al., Complete Activation of Porcine Oocytes Induced by the Sulfhydryl Reagent, Thimerosal, Biol of Reprod, 1997, 57:1123-1127.
[cited by applicant]
MacHaty, Zoltan, et al., Development of Early Porcine Embryos In Vitro and In Vivo, Biol of Reprod, 1998, 59:451-455.
[cited by applicant]
Madsen, Mette, et al., Molecular Characterization of the Haptoglobin Hemoglobin Receptor CD163, J Biol Chem, 2004, 279(49):51561-51567.
[cited by applicant]
Mansour, Suzanne L., et al., Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells. a general strategy for targeting mutations to non-selectable genes, Nature, 1988, 336:348-352.
[cited by applicant]
Mayes, M.A., et al., Parthenogenic Activation of Pig Oocytes by Protein Kinase Inhibition, Biol of Reprod, 1995, 53:270-275.
[cited by applicant]
McGrath, James and Solter, Davor, Nuclear Transplantation in the Mouse Embryo by Microsurgery and Cell Fusion, Science, 1983, 220(4603)1300-1302.
[cited by applicant]
Meng, X.J., Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development, Veterinary Microbiology, 2000, 74:309-329.
[cited by applicant]
Meng, Xiang-Jin, et al., Sequence comparison of open reading frames 2 to 5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus, J Gen Virology, 1995, 76:3181-3188.
[cited by applicant]
Mengeling, William L., et al., Identification and clinical assessment of suspected vaccine-related field strains of porcine reproductive and respiratory syndrome virus, AJVR, 1999, 60(3): 334-340.
[cited by applicant]
Shanmukhappa, Kumar, et al., Role of CD151, A tetraspantin, in porcine reproductive and respiratory syndrome virus infection, Virology Journal, 2007, 4:62.
[cited by applicant]
Shimozawa, Nobuhiro, et al., Abnormalities in Cloned Mice Are Not Transmitted to the Progeny, Genesis, 2002, 35:203-207.
[cited by applicant]
Snuder, Eric J., and Meulenberg, Janneke J.M., The molecular biology of arterivimses, J Gen Virology, 1998, 79:961-979.
[cited by applicant]
Soares, Mignel, P., Bach, Fritz H., Heme oxygenase-1: from biology to therapeutic potential, Trends in Molecular Medicine, 2009, 15(2):50-58.
[cited by applicant]
Stein, Michael, et al., Interleukin 4 Potently Enhances Murine Macrophage Mannose Receptor Activity: A Marker of Alternative Immmmmologie Macrophage Activation, J Exp Med, 1992, 176:287-292.
[cited by applicant]
Stephen, Sam L., et al., Scavenger Receptors and Their Potential as Therapeutic Targets in the Treatment of Cardiovascular Disease, International Journal of Hypertension, 2010, pp. 1-21.
[cited by applicant]
Stephenson, Rachel J., et al., Multiplex Serology for Common Viral Infections in Feral Pigs (
[cited by applicant]
Suarez, Paloma, et al., Open Reading Frame 5 of Porcine Reproductive and Respiratory Syndrome Virus as a Cause of Virus-Induced Apoptosis, J Virology, 1996, 70(5):2876-2882.
[cited by applicant]
Sulahian, Timothy H., et al., Human Monocytes Express CD163, Which is Unregulated by IL-10 and Identical to p155. Cytokine, 2000, 12(9):1312-1321.
[cited by applicant]
Sur, Jung-Hyang, et al., In Vivo Detection of Poreme Reproductive and Respiratory Syndrome Virus RNA by In Situ Hybridization at Different Times Postinfection, J Clinical Microbiology, 1996, 34(9):2280-2286.
[cited by applicant]
Sur, Jung-Hiyang, et al., Porcine Reproductive and Respiratory Syndrome Virus Replicates in Testienlat Germ Cells, Alters Spermatogenesis, and Induces Germ Cell Death by Apoptosis, J Virology, 1997, 71(12):9170-9179.
[cited by applicant]
Tait-Burkard, Christine, et al., Livestock: 2.0—genome editing for fitter, healthier, and more productive farmed animals, Genome Biology, 2018, 19(204):1-11.
[cited by applicant]
Terns, Michael P. and Terns, Rebecca M., CRISPR-Based Adaptive Imname Systems, Carr Opin Microbiol, 2011, 14(3):321-327.
[cited by applicant]
Trible, Benjamin R., et al., Recognition of the Different Structural Forms of the Capsid Protein Determines the Outcome following Infection with Porcine Circovirus Type 2, J Virology, 2012, 86(24):13508-13514.
[cited by applicant]
Vadori, M. and Cozzi, E., The immunological barriers to xenotransplantation, Tissue Antigens, 2015, 86:239-253.
[cited by applicant]
Van Breedam, Wander, et al., Porcine reproductive and respiratory syndrome virus entry into the porcine macrophage, J General Virology, 2010, 91:1659-1667.
[cited by applicant]
Van Breedam, Wander, et al., The M/GP5 Glycoprotein Complex of Porcine Reproductive and Respiratory Syndrome Virus Binds the Sialoadhesin Receptor in a Siabe Acid-Dependent Manner, PLoS Pathogens, 2010, 6(1):1-11.
[cited by applicant]
Van Den Heuvel, Michel M., et al., Regulation of CD163 on human macrophages: cross-linking of CD163 induces signaling and activation, J Leukocyte Biology, 1999, 66(5):858-866.
[cited by applicant]
Van Den Hoff, Maurice J.B., et al., Electroporation in ‘intracellular’ buffer increases cell survival, Nucleic Acids Research, 1992, 20(11):2902.
[cited by applicant]
Vanderheiden, Nathalie, et al., Involvement of Sialoadhesin in Entry of Porcine Reproductive and Respiratory Syndrome Virus into Porcine Alveolar Macrophages, 2003, J Virology, 77(15):8207-8215.
[cited by applicant]
Van Gorp, Hanne, et al., Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy, 2010, 47:1650-1660.
[cited by applicant]
Van Gorp, Hane, et al., Identification of the CD163 Protein Domains Involved in Infection of the Porcine Reproductive and Respiratory Syndrome Virus, 2010, J Virology, 84(6):3101-3105.
[cited by applicant]
Van Gorp, Hanne, et al., Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus, J General Virology, 2008, 89:2943-2953.
[cited by applicant]
Vinson, Mary, et al., Characterization of the Sialic Acid-binding Site in Sialoadhesin by Site-directed Mutagenesis, J Biological Chemistry, 1996, 271(16):9267-9272.
[cited by applicant]
Walters, Eric M. and Prather, Randall S., Advancing Swine Models for Human Health and Diseases, Missouri Medicine, 2013, 110(3):212-215.
[cited by applicant]
Wang, Haoyi, et al., One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering, Cell, 2013, 153(4):910-918.
[cited by applicant]
Watanabe, Masahito, et al., Knockout of exogenous EGFP gene in porcine somatic cells using zine-finger nucleases, Biochem and Biophysical Research Communications, 2010, 402:14-18.
[cited by applicant]
Welch, Siao-Kun and Calvert, Jay G., A brief review of CD163 and its role in PRRSV infection, Virus Research, 2010, 154:98-103.
[cited by applicant]
Wells, Kevin D., et al., Replacement of Porcine CD163 Scavenger Receptor Cysteine-Rich Domain 5 with a CD163-Like Homolog Confers Resistance of Pigs to Genotype 1 but Not Genotype 2 Porcine Reproductive and Respiration …
[cited by applicant]
Wensvoort, O., et al., Mystery swine disease in the Netherlands: The isolation of Lelystad virus, Veterinary Quarterly, 1991, 13(3):121-130.
[cited by applicant]
Whitworth, Kristin M., et al., Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus, Nature Biotechnology, 2016, 34(1):20-22.
[cited by applicant]
Whitworth, K. M., et al., “Disruption the Sialoadhesin and CD163 Genes to Create Pigs Resistant to PRRSV Infectivity,” Abstract, Swine in Biomedical Research in Chicago, Illinois, Jul. 17-19, 2011, S1-25, pp. 39-40.
[cited by applicant]
Whitworth, Kristin M., et al., Method of Oocyte Activation Affects Cloning Efficiency in Pigs, Mol Reprod Dev, 2002, 76:1.11.
[cited by applicant]
Whitworth, Kristin M., et al., Activation Method Does Not Alter Abnormal Placental Gene Expression and Development in Cloned Pigs, Mol Reprod Dev, 2010, 77:1016-1030.
[cited by applicant]
Whitworth, K. M., et al., “Pigs Resistant to PRRSV Infectivity. ” Poster Presented at Swine in Biomedical Research in Chicago, Illinois, Jul. 18, 2011, 1 page.
[cited by applicant]
Whitworth, Kristin M., et al., Scriptaid Corrects Gene Expression of a Few Aberrantly Reprogrammed Transcripts in Nuclear Transfer Pig Blastocyst Stage Embryos, Cellular Reprogramming, 2011, 13(3):191-204.
[cited by applicant]
Whitworth, Kristin M. and Prather, Randall S., Gene editing as applied to prevention of reproductive porcine reproductive and respiratory syndrome, Mol Reprod Dev, 2017, 33.
[cited by applicant]
Whitworth, Kristin M., et al., Use of the CRISPR/Cas9 System to Produce Genetically Engineered Pigs from In Vitro-Derived Oocytes and Embryos, Biol of Reproduction, 2014, 91(3):78, 1-13.
[cited by applicant]
Whitworth, K. M., et al., “Use of the CRISPR/Cas9 System to Produce Pigs With a Genetically Modified CD163 Gene by Using Somatic Cell Nuclear Transfer of In Vitro Derived Oocytes,” Abstract, Swine in Biomedical Research…
[cited by applicant]
Whitworth, Kristin M., et al., Use of the CRISPR/Cas9 system to produce pigs with a genetically modified CD163 gene by somatic cell unclear transfer of in vitro derived oocytes, Division of Animal Science, University of…
[cited by applicant]
Whitworth, K.M., et al., Gene editing of CD163 protects pigs from PRRSV infectivity, Abstracts from the UC Davis Transgenic Animal Research Conference XI, Aug. 13-17, 2017, Transgenic Res, 2018, 27:473-474.
[cited by applicant]
Whyte, Jeffrey J., et al., Gene Targeting With Zinc Finger Nucleases to Produce Cloned eGFP Kouckout Pigs, Mol Reprod Dev, 2011, 78(1):1-3.
[cited by applicant]
Whyte, Jeffrey and Prather, Randall S., Genetic modifications of pigs for medicine and agriculture, Mol Reprod Dev, 2011, 78(10-11):879-891.
[cited by applicant]
Wiedenheft, Blake, et al., RNA-guided genetic silencing systems in bacteria and archaea, Nature, 2012, 482:331-338.
[cited by applicant]
Wills. R. W., et al., Porcine reproductive and respiratory syndrome virus: Persistent infection, Veterinary Microbiology 1997, 55:231-240.
[cited by applicant]
Winckler, C. and Willen, S., The Reliability and Repeatability of a Lameness Scoring System for Use as an Indicator of Welfare in Dairy Cattle, Acta Agric Scand Sect A, Animal Sci Suppl, 2001, 30:103-107.
[cited by applicant]
Wissink, E.H.J., et al., Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus, Arch Virol, 2003, 148:177-187.
[cited by applicant]