Tobramycin compound-copolymer conjugates and derivative compositions
Disclosed herein are compositions comprising tobramycin or a tobramycin derivative that exhibit improvements in permeation of corneal cells, retention in corneal cells, or both. Such compositions can comprise tobramycin or tobramycin derivative(s) complexed to an agent which facilitates improved permeation of corneal cells, retention in corneal cells, or both. The invention also relates to a process of preparing such compositions.
1. A method of treating an infection caused by an infective agent in a mammalian eye comprising administering to the mammalian eye an ophthalmologically suitable pharmaceutical formulation, which formulation comprises a complexed compound, the complexed compound comprising:
(a) an effective amount of tobramycin and
(b) an ophthalmologically safe copolymer complexing agent consisting of polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft co-polymer (PCL-PVAc-PEG),
wherein the ophthalmologically safe copolymer complexing agent forms a stable complex with the tobramycin and the ophthalmologically safe copolymer complexing agent detectably promotes the uptake of the tobramycin by corneal cells, the retention of the tobramycin by corneal cells, or both the uptake and retention of the tobramycin by corneal cells, as compared to tobramycin in non-complexed form.
2. The method of claim 1 , wherein the PCL-PVAc-PEG is present in the formulation in an amount of about 0.3% w/v to about 5% w/v.
3. The method of claim 2 , wherein the PCL-PVAc-PEG is present in the formulation in an amount of about 1% w/v to about 3% w/v.
4. The method of claim 2 , wherein the tobramycin is present in the formulation in an amount of about 0.3% to about 3% w/v, and the PCL-PVAc-PEG is present in the formulation in an amount of about 0.3% w/v to about 3% w/v.
5. The method of claim 1 , wherein the rate of corneal cell permeation for the tobramycin is detectably or significantly faster than the rate of corneal cell permeation for the tobramycin in non-complexed form.
6. The method of claim 5 , wherein the rate of corneal cell permeation for the tobramycin is at least about 15% faster than the rate of corneal cell permeation for tobramycin in non-complexed form, after about 60 minutes from administration to corneal cells.
7. The method of claim 4 , wherein the concentration of the tobramycin permeating the surface of the cornea 15 minutes after administration is at least about 15% greater than the corresponding concentration of the tobramycin permeating the surface of the cornea in a composition consisting of tobramycin at 0.3% w/v, benzalkonium chloride at 0.01% w/v, boric acid, sodium sulfate, sodium chloride, tyloxapol, sodium hydroxide and/or sulfuric acid, and purified water 15 minutes after administration.
8. The method of claim 1 , wherein an effective amount of the formulation prevents detectable or significant growth of bacteria for a period of at least 6 hours after administration.