Airborne pathogen simulants and mobility testing
Airborne pathogen mobility and the airborne mobility of respiratory droplets, such as saliva, and testing thereof, can be monitored by tracking detectable compounds and measuring concentrations. A display can be presented including a building layout and simulant concentrations released and collected at various locations within a space.
1. A method of testing and evaluating a building space for possible airborne pathogen travel, the method comprising:
releasing a simulant at a first release location within the building space, wherein the simulant comprises water, a DNA taggant, and a carrier, and wherein the simulant is characterized in having a behavior suitable for simulating human emission of a pathogen;
collecting a portion of released simulant at a first collection location within the building space, wherein the first release location and the first collection location are separated by building infrastructure of the building space and wherein the first release location and the first collection location are such that measurable airflow occurs from the first release location to the first collection location; and
displaying movement of the simulant, comprising:
(a) receiving information of a first amount of the simulant at the first release location and of a second amount of the simulant detected the first collection location; and
(b) generating a report displaying a first graphic element representing the first amount of the simulant at the first release location and displaying a second graphic element representing the second amount of the simulant at the first collection location in a map comprising the first release location and the first collection location.
2. The method of claim 1 , wherein the pathogen is SARS-Cov-2.
3. The method of claim 1 , wherein the carrier comprises polysaccharides, proteins, salt, or a combination thereof.
4. The method of claim 3 , further comprising one or more additional carriers to form a saliva simulant solution containing around 99% H 2 O, and around 1% of a mixture of the polysaccharides, the proteins, the salt, or the combination thereof, and DNA taggants.
5. The method of claim 1 , wherein the simulant comprises dispensed liquid particles that transport through air sized from about 0.01 μm to about 100 μm, formed by binding the DNA taggant to a carrier polysaccharide and/or one or more protein.
6. The method of claim 1 , wherein the map comprises a heat map.
7. The method of claim 1 , wherein the map comprises elements representing simulant concentrations overlaid on a building layout.
8. The method of claim 1 , wherein the simulant has a characteristic that matches or simulates saliva.
9. A method of testing and evaluating a building space for possible airborne pathogen travel, the method comprising:
releasing a simulant at a first release location within the building space, wherein the simulant comprises water, a DNA taggant, and a carrier, and wherein the simulant is characterized in having a behavior suitable for simulating human emission of a pathogen;
collecting a portion of released simulant at a first collection location within the building space, wherein the first release location and the first collection location are separated by building infrastructure of the building space and wherein the first release location and the first collection location are such that measurable airflow occurs from the first release location to the first collection location
mapping a plurality of collection locations;
mapping a plurality of release locations;
releasing a selected DNA taggant at a release location;
collecting droplet and/or aerosol sample at a collection location of the plurality of collection locations; and
quantifying DNA concentrations at collection locations using qPCR.
10. The method of claim 9 , wherein at least some of the plurality of release locations are mobile and simulate dispersing while moving.
11. The method of claim 9 , wherein releasing comprises releasing a plurality of releases using a plurality of DNA taggants or combinations thereof.
12. A method of testing and evaluating a building space for possible airborne pathogen travel, the method comprising:
releasing a simulant at a first release location within the building space, wherein the simulant comprises water, a DNA taggant, and a carrier, and wherein the simulant is characterized in having a behavior suitable for simulating human emission of a pathogen; and
collecting a portion of released simulant at a first collection location within the building space, wherein the first release location and the first collection location are separated by building infrastructure of the building space and wherein the first release location and the first collection location are such that measurable airflow occurs from the first release location to the first collection location,
wherein the simulant has a characteristic that matches or simulates saliva, and wherein the characteristic is an evaporation rate.
13. The method of claim 12 , wherein the pathogen is SARS-Cov-2.
14. The method of claim 12 , wherein the carrier comprises polysaccharides, proteins, salt, or a combination thereof, wherein the simulant comprises dispensed liquid particles that transport through air sized from about 0.01 μm to about 100 μm, formed by binding the DNA taggant to a carrier polysaccharide and/or one or more protein, and wherein one or more additional carriers form a saliva simulant solution containing around 99% H 2 O, and around 1% of a mixture of the polysaccharides, the proteins, the salt, or the combination thereof, and DNA taggants.
15. The method of claim 12 , further comprising:
mapping a plurality of collection locations;
mapping a plurality of release locations;
releasing a selected DNA taggant at a release location;
collecting droplet and/or aerosol sample at a collection location of the plurality of collection locations; and
quantifying DNA concentrations at collection locations using qPCR.
16. The method of claim 15 , wherein at least some of the plurality of release locations are mobile and simulate dispersing while moving.
17. The method of claim 15 , wherein releasing comprises releasing a plurality of releases using a plurality of DNA taggants or combinations thereof.
18. A method of testing and evaluating a building space for possible airborne pathogen travel, the method comprising:
releasing a simulant at a first release location within the building space, wherein the simulant comprises water, a DNA taggant, and a carrier, and wherein the simulant is characterized in having a behavior suitable for simulating human emission of a pathogen; and
collecting a portion of released simulant at a first collection location within the building space, wherein the first release location and the first collection location are separated by building infrastructure of the building space and wherein the first release location and the first collection location are such that measurable airflow occurs from the first release location to the first collection location,
wherein the simulant has a characteristic that matches or simulates emission by a human of a human emission and wherein the characteristic is an evaporation rate of the human emission.
19. The method of claim 18 , wherein the human emission is one or more of a human aerosol emission, a human droplet emission, a human droplet emission followed by at least partial evaporation to become aerosolized, and/or a combination thereof.
20. The method of claim 18 , further comprising:
mapping a plurality of collection locations;
mapping a plurality of release locations;
releasing a selected DNA taggant at a release location;
collecting droplet and/or aerosol sample at a collection location of the plurality of collection locations; and
quantifying DNA concentrations at collection locations using qPCR.