Coronavirus vaccine
The present invention is directed to a nucleic acid suitable for use in treatment or prophylaxis of an infection with a coronavirus, preferably with a Coronavirus SARS-CoV-2, or a disorder related to such an infection, preferably COVID-19. The present invention is also directed to compositions, polypeptides, and vaccines. The compositions and vaccines preferably comprise at least one of said nucleic acid sequences, preferably nucleic acid sequences in association a lipid nanoparticle (LNP). The invention is also directed to first and second medical uses of the nucleic acid, the composition, the polypeptide, the combination, the vaccine, and the kit, and to methods of treating or preventing a coronavirus infection, preferably a Coronavirus infection.
1. A composition comprising a mRNA comprising:
(a) at least one coding sequence encoding a SARS-CoV-2 spike protein (S) at least 95% identical to SEQ ID NO: 10 that is a pre-fusion stabilized spike protein (S_stab) comprising K986P and V987P stabilizing mutations and H69del, V70del, S477N, T478K, E484A, N501Y, and D614G amino acid substitutions relative to SEQ ID NO: 10;
(b) at least one heterologous untranslated region (UTR); and
(c) at least one pharmaceutically acceptable carrier,
wherein the mRNA is complexed or associated with lipid nanoparticles (LNP) and wherein the LNP comprises:
(i) at least one cationic lipid;
(ii) at least one neutral lipid;
(iii) at least one steroid or steroid analogue; and
(iv) at least one PEG-lipid,
wherein (i) to (iv) are in a molar ratio of about 20-60% cationic lipid, 5-25% neutral lipid, 25-55% sterol, and 0.5-10% PEG-lipid.
2. The composition of claim 1 , wherein the mRNA comprises at least one poly(A) sequence comprising 30 to 200 adenosine nucleotides and a 5′-cap structure.
3. The composition of claim 2 , wherein the at least one PEG lipid is present in the LNP in a molar ratio of about 0.5% to 5%.
4. The composition of claim 1 , wherein the at least one coding sequence of the mRNA has a G/C content of at least about 50%.
5. The composition of claim 1 , wherein the mRNA comprises a sequence at least 80% identical to SEQ ID NO: 137.
6. The composition of claim 1 , wherein the at least one heterologous untranslated region is selected from at least one heterologous 5′-UTR and/or at least one heterologous 3′-UTR.
7. The composition of claim 1 , wherein the mRNA comprises a nucleotide analog.
8. The composition of claim 7 , wherein the mRNA comprises a 1-methylpseudouridine substitution.
9. The composition of claim 8 , wherein 100% of the uracil positions in the coding sequence are replaced with 1-methylpseudouridine.
10. The composition of claim 1 , wherein the mRNA has an RNA integrity of at least about 50%.
11. The composition of claim 1 , wherein the mRNA is a purified mRNA that has been purified by RP-HPLC and/or TFF.
12. The composition of claim 11 , wherein the mRNA is a purified mRNA that hasbeen purified by RP-HPLC and/or TFF and comprises about 5%, 10%, or 20% less double stranded RNA side products as an RNA that has not been purified with RP-HPLC and/or TFF.
13. The composition of claim 1 , further comprising a lyoprotectant, comprising sucrose.
14. The composition of claim 1 , wherein at least 80% of the mRNA is intact at least about two weeks after storage as a liquid at temperatures of about 5° C.
15. The composition of claim 1 , wherein the composition comprises less than about 20% free mRNA.
16. The composition of claim 1 , wherein the LNPs have a mean diameter of from about 60 nm to 200 nm.
17. The composition of claim 1 , wherein the composition has a lipid to RNA molar ratio (N/P ratio) of from about 2 to about 12.
18. The composition of claim 1 , wherein the at least one coding sequence encoding a SARS-CoV-2 protein is at least 95% identical to SEQ ID NO: 10 and additionally comprises Y144del P681H, H655Y and Q493R amino acid changes relative to SEQ ID NO: 10.
19. The composition of claim 1 , wherein the at least one coding sequence encoding a SARS-CoV-2 S protein is at least 95% identical to SEQ ID NO: 10 and additionally comprises a G446S and/or K417N amino acid change relative to SEQ ID NO: 10.
20. The composition of claim 9 , wherein the LNP comprises:
(i) at least one cationic lipid;
(ii) at least one neutral lipid comprising 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC);
(iii) at least one sterol comprising cholesterol; and
(iv) at least one PEG-lipid,
wherein (i) to (iv) are in a molar ratio of about 45-65% cationic lipid, 5-25% neutral lipid, 25-55% sterol, and 0.5-5% PEG-lipid.
21. The composition of claim 20 , wherein the at least one PEG-lipid comprises PEG-DMG or PEG-cDMA.
22. The composition of claim 21 , wherein the at least one PEG-lipid comprises PEG-2000-DMG.
23. The composition of claim 22 , wherein the composition has a lipid to RNA molar ratio (N/P ratio) of from about 2 to about 12.
24. The composition of claim 23 , wherein the LNPs have a mean diameter of from about 60 nm to 200 nm.
25. The composition of claim 24 , wherein the mRNA comprises at least one poly(A) sequence comprising 30 to 200 adenosine nucleotides and a 5′-cap structure.
26. A composition comprising a mRNA comprising:
(a) at least one coding sequence encoding a SARS-CoV-2 spike protein (S) at least 90% identical to SEQ ID NO: 10 that is a pre-fusion stabilized spike protein (S_stab) comprising K986P and V987P stabilizing mutations and K417N, S477N, T478K, E484A, D614G, N501Y, P681H, H655Y and Q493R amino acid changes relative to SEQ ID NO: 10;
(b) at least one heterologous untranslated region (UTR); and
(c) at least one pharmaceutically acceptable carrier,
wherein the mRNA is complexed or associated with lipid nanoparticles (LNP) and wherein the LNP comprises:
(i) at least one cationic lipid;
(ii) at least one neutral lipid;
(iii) at least one steroid or steroid analogue; and
(iv) at least one PEG-lipid,
wherein (i) to (iv) are in a molar ratio of about 20-60% cationic lipid, 5-25% neutral lipid, 25-55% sterol, and 0.5-10% PEG-lipid.
27. A method of inducing an immune response against SARS-CoV-2 in a subject comprising administering to the subject an effective amount of a composition according to claim 20 .