Granted Patent B2

US 12,704,510 · App. 17/527,256 · Granted Aug 11, 2026

Inventors: Rajesh K. Mehra (Parsippany, NJ); Vincent Chiang (Parsippany, NJ); Kenneth P. Aron (Parsippany, NJ); Asher Krell (Parsippany, NJ)

Assignee: Zoetis Services LLC

G01N33/553B82Y15/00

Abstract

Claims (41)

1 . A localized surface plasmon resonance (LSPR) analyte detection device comprising:

a plurality of detection conjugates, wherein the conjugates comprise composite metallic nanostructures coupled to binding partners that are capable of specifically binding to a target analyte, wherein the composite metallic nanostructures comprise a silver core and a gold coating;

a surface containing a metallic nanolayer; and

a plurality of capture molecules, wherein the capture molecules are immobilized on the metallic nanolayer and are capable of specifically binding to the target analyte.

2 . A localized surface plasmon resonance (LSPR) analyte detection device comprising:

a plurality of detection conjugates, wherein the conjugates comprise composite metallic nanostructures coupled to target analytes, wherein the composite metallic nanostructures comprise a silver core and a gold coating;

a surface containing a metallic nanolayer; and

a plurality of capture molecules, wherein the capture molecules are immobilized on the metallic nanolayer and are capable of specifically binding to the target analytes.

3 . The LSPR analyte detection device of claim 1 , wherein the composite metallic nanostructures are spherical nanoparticles and have a diameter of about 5 nm to about 200 nm.

4 . The LSPR analyte detection device of claim 1 , wherein the composite metallic nanostructures are spherical nanoparticles and have a diameter of about 10 nm to about 100 nm.

5 . The LSPR analyte detection device of claim 1 , wherein the composite metallic nanostructures are nanoplates with an edge length of about 10 nm to about 800 nm and a thickness of about 1 nm to about 100 nm.

6 . The LSPR analyte detection device of claim 1 , wherein the plurality of detection conjugates is in the form of a lyophilized pellet or bead.

7 . The LSPR analyte detection device of claim 1 , wherein the surface is a chip, a well, a bead, or a wall, cover, and/or bottom of a cuvette.

8 . The LSPR analyte detection device of claim 1 , wherein the metallic nanolayer is a metallic film.

9 . The LSPR analyte detection device of claim 8 , wherein the metallic film comprises gold, silver, copper, platinum, palladium, cadmium, zinc or a composite thereof.

10 . The LSPR analyte detection device of claim 8 , wherein the metallic film comprises gold.

11 . The LSPR analyte detection device of claim 1 , wherein the metallic nanolayer comprises a plurality of metallic nanostructures immobilized on the surface.

12 . The LSPR analyte detection device of claim 11 , wherein the plurality of metallic nanostructures comprise gold, silver, copper, platinum, palladium, cadmium, zinc or a composite thereof.

13 . The LSPR analyte detection device of claim 11 , wherein the plurality of metallic nanostructures are gold nanostructures.

14 . The LSPR analyte detection device of claim 1 , wherein the composite nanostructures have a geometry selected from spherical nanoparticles, pyramidal nanoparticles, hexagonal nanoparticles, nanoshells, nanotubes, nanorods, nanodots, nanoislands, nanowires, or combinations thereof.

15 . The LSPR analyte detection device of claim 1 , wherein the binding partners and/or capture molecules are antibodies, antigens, polypeptides, polynucleotides, nucleoproteins, aptamers, ligands, receptors, or haptens.

16 . The LSPR analyte detection device of claim 1 , wherein the binding partners are antibodies that recognize a first epitope of a target analyte and the capture molecules are different antibodies that recognize a second epitope of a target analyte.

17 . The LSPR analyte detection device of claim 2 , wherein the capture molecules are antibodies, antigens, polypeptides, polynucleotides, nucleoproteins, aptamers, ligands, receptors, or haptens.

18 . The LSPR analyte detection device of claim 1 , wherein the target analyte is a marker or antigen associated with an infectious disease, physiological state, or pathological condition.

19 . The LSPR analyte detection device of claim 1 , wherein the target analyte is canine heartworm, feline leukemia virus, canine parvovirus, C-reactive protein, Giardia lamblia, Ehrlichia antigen or antibody, Borrelia antigen or antibody, Anaplasma antigen or antibody, a cancer antigen, a cardiac marker antigen, thyroid stimulating hormone, thyroxine, troponin, or brain natriuretic peptide.

20 . A method of detecting a target analyte in a sample based on localized surface plasmon resonance (LSPR), the method comprising:

mixing the sample with a plurality of detection conjugates, wherein the conjugates comprise composite metallic nanostructures coupled to binding partners that are capable of specifically binding to the target analyte if present in the sample to form analyte-detection conjugate complexes, wherein the composite metallic nanostructures comprise a silver core and a gold coating;

contacting the mixture with a surface containing a metallic nanolayer, wherein a plurality of capture molecules are immobilized on the metallic nanolayer and are capable of specifically binding to the target analyte if present in the sample;

exposing the surface to a light source at a wavelength range within the ultraviolet-visible-infrared spectrum; and

measuring an optical signal from the surface, wherein a change in the optical signal indicates the presence of the target analyte in the sample.

21 . The method of claim 20 , wherein the optical signal is reflectance, an absorbance spectrum, scattering spectrum, or an emission spectrum.

22 . The method of claim 20 , wherein the change in the optical signal comprises a spectral peak wavelength shift.

23 . The method of claim 20 , wherein the presence of nanogram quantities of the target analyte is detected.

24 . The method of claim 20 , wherein the presence of picogram quantities of the target analyte is detected.

25 . The method of claim 20 , wherein the presence of femtogram quantities of the target analyte is detected.

26 . The method of claim 20 , wherein the surface is the walls and bottom of a cuvette incorporated into a centrifugal rotor.

27 . The method of claim 20 , wherein the metallic nanolayer is a metallic film.

28 . The method of claim 27 , wherein the metallic film comprises gold.

29 . The method of claim 20 , wherein the metallic nanolayer comprises a plurality of metallic nanostructures immobilized on the surface.

30 . The method of claim 29 , wherein the plurality of metallic nanostructures are gold nanostructures.

31 . The method of claim 20 , wherein the composite nanostructures have a geometry selected from spherical nanoparticles, pyramidal nanoparticles, hexagonal nanoparticles, nanotubes, nanoshells, nanorods, nanoislands, nanodots, nanowires, or combinations thereof.

Assignments (3)

Continuity (5)

References Cited (251)